Date published: 2025-9-10
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BrdU Antibody (IIB5) - Immunofluorescence - Image 10222
BrdU 抗体 (IIB5): sc-32323. 甲醇固定的 BrdU 标记的 HeLa 细胞免疫荧光染色, 显示核定位.
BrdU Antibody (IIB5) - Immunofluorescence - Image 10222
BrdU 抗体 (IIB5): sc-32323. 甲醇固定的 BrdU 标记的 HeLa 细胞免疫荧光染色, 显示核定位.
BrdU Antibody (IIB5) - Flow Cytometry - Image 9913
BrdU 抗体 (IIB5) PE: sc-32323 PE. 对固定和通透的未处理 (蓝色虚线直方图) 和经 BrdU 处理 (橙色实线直方图) 的 K-562 细胞进行胞内 FCM 分析. 绿线直方图代表同型对照, 正常小鼠 IgG1: sc-2866.
BrdU 抗体 (IIB5): sc-32323. 甲醇固定的 BrdU 标记的 HeLa 细胞免疫荧光染色, 显示核定位.

BrdU 抗体 (IIB5): sc-32323

4.5(28)
寫評論提問

说明书
  • BrdU 抗体 IIB5 是小鼠单克隆 IgG1 κ，BrdU抗体, 在253篇文献中引用，规格为200 µg/ml
  • 与Bromodeoxyuridine (BrdU)结合，与BSA结合
  • BrdU 抗体 (IIB5) 推荐用于 IF, IHC(P) 和 FCM，检测 BrdU, a proliferation marker incorporated into newly synthesized DNA during S-phase of a cell cycle,
  • 识别标记了BrdU的细胞的变性DNA中的BrdU; 可能与 iododeoxyuridine (IrdU)产生交联反应
  • BrdU抗体（IIB5）可与琼脂糖结合用于IP；与HRP结合用于IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取BrdU (IIB5): sc-32323的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是BrdU Antibody (IIB5) for IHC(P) applications. 的首选辅助检测试剂，这些试剂现与BrdU Antibody (IIB5) 打包提供（请参阅下面的订购信息）。

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描述
说明书与实验方案
研究信息

関連項目

BrdU抗体（IIB5）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和流式细胞术（FCM）检测非物种特异性应用中的BrdU蛋白。抗BrdU抗体（IIB5）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。卤代嘧啶类似物溴脱氧尿苷（BrdU）在DNA合成中起着至关重要的作用，在细胞周期的S期结合到新合成的DNA链中，这使得BrdU成为评估活跃进行DNA复制的细胞比例的宝贵工具。这种结合使研究人员能够量化DNA合成并研究细胞过程，例如姐妹染色单体交换和新生DNA分离。BrdU (IIB5)抗体与盐酸变性或核酸酶消化后的单链DNA中暴露的BrdU特异性结合，这对于精确检测至关重要。为了获得最佳结果，建议对用福尔马林等交联剂固定的组织或细胞进行蛋白酶抗原回收，尽管这可能会影响BrdU免疫检测的特异性。BrdU (IIB5) 单克隆抗体可显示细胞质染色，在免疫组织化学和免疫细胞化学应用中提供清晰的可视化效果，从而有助于更深入地了解细胞增殖和DNA合成动态。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

BrdU 抗体 (IIB5) 参考文献:

  1. 前核糖体 RNA 在转录部位的渗透细胞中进行加工。  |  Stanek, D., et al. 2000. Eur J Cell Biol. 79: 202-7. PMID: 10777112
  2. 持续暴露于溴脱氧尿苷（BrdU）会对人类乳腺癌和膀胱癌细胞系的细胞周期进展产生不同影响。  |  Diermeier, S., et al. 2004. Cell Prolif. 37: 195-206. PMID: 15030553
  3. 溴脱氧尿苷可提高人骨髓干细胞的多潜能性。  |  Qu, TY., et al. 2004. Restor Neurol Neurosci. 22: 459-68. PMID: 15798364
  4. 用单克隆抗溴脱氧尿苷对细胞和组织样本进行双重免疫细胞化学标记。  |  Magaud, JP., et al. 1989. J Histochem Cytochem. 37: 1517-27. PMID: 2476478
  5. 用单克隆抗体检测肿瘤和染色体中的溴脱氧尿苷。  |  Morstyn, G., et al. 1983. J Clin Invest. 72: 1844-50. PMID: 6355188
  6. 使用 5-溴-2'-脱氧尿苷抗体分离含有切除修复位点的 DNA 序列。  |  Cohn, SM. and Lieberman, MW. 1984. J Biol Chem. 259: 12456-62. PMID: 6490625
  7. 溴脱氧尿苷浸润的结直肠肿瘤组织的盐酸变性。  |  Williamson, K., et al. 1994. Cytometry. 15: 162-8. PMID: 8168403
  8. 蛋白酶抗原恢复会降低福尔马林固定组织中溴脱氧尿苷检测的特异性。  |  Bak, PM. and Panos, RJ. 1997. J Histochem Cytochem. 45: 1165-70. PMID: 9267477
  9. 小鸡胚胎的高热：HSP 和发育缺陷的可能机制。  |  Buckiová, D., et al. 1998. Int J Dev Biol. 42: 737-40. PMID: 9712529

订购信息

产品名称产品编号规格价格数量收藏夹

BrdU 抗体 (IIB5)

sc-32323
200 µg/ml
$316.00

BrdU (IIB5): m-IgG Fc BP-HRP 套装

sc-526681
200 µg Ab; 10 µg BP
$354.00

BrdU (IIB5): m-IgG1 BP-HRP 套装

sc-532054
200 µg Ab; 20 µg BP
$354.00

BrdU 抗体 (IIB5) AC

sc-32323 AC
500 µg/ml, 25% agarose
$416.00

BrdU 抗体 (IIB5) HRP

sc-32323 HRP
200 µg/ml
$316.00

BrdU 抗体 (IIB5) FITC

sc-32323 FITC
200 µg/ml
$330.00

BrdU 抗体 (IIB5) PE

sc-32323 PE
200 µg/ml
$343.00

BrdU 抗体 (IIB5) Alexa Fluor® 488

sc-32323 AF488
200 µg/ml
$357.00

BrdU 抗体 (IIB5) Alexa Fluor® 546

sc-32323 AF546
200 µg/ml
$357.00

BrdU 抗体 (IIB5) Alexa Fluor® 594

sc-32323 AF594
200 µg/ml
$357.00

BrdU 抗体 (IIB5) Alexa Fluor® 647

sc-32323 AF647
200 µg/ml
$357.00

BrdU 抗体 (IIB5) Alexa Fluor® 680

sc-32323 AF680
200 µg/ml
$357.00

BrdU 抗体 (IIB5) Alexa Fluor® 790

sc-32323 AF790
200 µg/ml
$357.00

I wonder that I can use this antibody for Immunohistochemistry(IHC) in frozen section using OCT. I'm using the mouse injected with Brdu solution at E13.5 or E16.5.

Asked by: qwa0427_
Thank you for your question. BrdU Antibody (IIB5) is recommended for detection of BrdU, a proliferation marker incorporated into newly synthesized DNA during S-phase of a cell cycle, by IF, IHC(P) and FCM. It can be used in IHC applications with frozen tissue sections as well.
Answered by: Tech Support Europe
Date published: 2022-04-21

Should I use Anti-BrdU Antibody (IIB5) or Anti-BrdU Antibody MoBU 1 to detect BrdU incorporated cells in the hippocampus of diabetic rats, for IHC-P?

Asked by: gee2
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext.1, allowing for a more interactive discussion of this and other related questions
Answered by: Blake J
Date published: 2021-11-23

What's the clone of this antibody? is it Bu20a? Does it cross react with EdU?

Asked by: swordfish123
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext.1, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2021-11-15

Is it absolutely necessary to do a denaturing step? I could not denature as I want to pull down the DNA-RNA hybridization.

Asked by: yizhiyang
Thank you for your question. This antibody is recommended for detection of BrdU, a proliferation marker incorporated into newly synthesized DNA during S-phase of a cell cycle, by IF, IHC(P) and FCM. We didn't test it on pulling down the DNA-RNA hybridization.
Answered by: Technical Support 12
Date published: 2021-10-08

What is the recommended protocol for blocking sc-32323 agarose beads?

Asked by: ip_maker
Thank you for your question. Please contact your local distributor or technical service for technical assistance for this antibody.
Answered by: Tech Service
Date published: 2020-02-04

Can you use Trizol to isolate nucleic acids from agarose conjugated antibodies?

Asked by: GmoFlo
Thank you for your question. TRIzol is a biochemical used in the extraction of DNA, RNA, and proteins from cells,. We have not tried to isolate nucleic acids from AC conugated antibodies, so we are unable to provide this information.
Answered by: Tech Service
Date published: 2019-03-22

Does it react with Iododeoxyuridine (IdU)? I want to use it for recognizing IdU not BrdU.

Asked by: moz93
Thank you for your question. No, this antibody does not cross react with IdU.
Answered by: Technical Support
Date published: 2019-02-01

Hi Is this nuclease free? I want to use it for RNA pull down.

Asked by: ohsamboss
Thank you for your question. We do not routinely test for this and can not guarantee that the antibody in stock for our mouse monoclonal BrdU Antibody (IIB5): sc-32323 is nuclease free.
Answered by: Technical Support
Date published: 2017-02-15
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Rated 5 out of 5 by from NiceIt worked for IF on PFA-fixed cryosections, after 1hr treatment with HCl 1M.
Date published: 2020-02-18
Rated 5 out of 5 by from Worked excellent for immunoflorescence stainingI got this as a sample product and I am happy it worked extremely well for standardizing my protocol.
Date published: 2019-03-22
Rated 5 out of 5 by from Great featureI bought this couple month ago and its working very nicely
Date published: 2018-06-08
Rated 5 out of 5 by from Great for co-IPIt is a great antibody for co-IP, and I highly recommend it.
Date published: 2018-01-13
Rated 5 out of 5 by from Good detection of S phase in embryosWe have used this antibody to detect BrDU incorporation during embryonic S phase in mouse two-cell embryos, using IF. We have obtained very good quality nuclear staining!
Date published: 2018-01-08
Rated 5 out of 5 by from Ottimo anticorpoHo utilizzato questo anticorpo per l'immunoprecipitazione di RNA marcati con BrU è ottenuto un ottimo risultato, valutato sia per pcr convenzionale che real-time.
Date published: 2017-11-10
Rated 5 out of 5 by from Works very good on IF of tissue samplesWe used this product for the IF detection of BrdU labeled small intestine proliferative cells in the frozen sections. 1:200 dilution was used.
Date published: 2017-10-04
Rated 1 out of 5 by from BrdU beads quality varies lot to lotI have bought BrdU AC beads for 5 + years and approximately 50% of the lots do not IP efficiently. Santa Cruz needs to improve their quality control so we do not waste time and money with lot variability.
Date published: 2017-08-21
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BrdU 抗体 (IIB5) is rated 4.5 out of 5 by 28.
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