Date published: 2025-9-7

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BMP-4 抗体 (3H2.3): sc-12721

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说明书
  • BMP-4抗体(3H2.3)是小鼠单克隆IgG2b κ, 在39篇文献中引用,规格为200 µg/ml
  • 免疫mouse物种的重组BMP-4
  • 抗-BMP-4 抗体 (3H2.3) 推荐用于 WB, IP 和 IF,检测mouse, rat 和human 来源的 presursor and mature BMP-4
  • 抗BMP-4抗体(3H2.3)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取BMP-4 (3H2.3): sc-12721免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2b BP-HRP">m-IgG2b BP-HRP是BMP-4 Antibody (3H2.3) 适用于 WB 应用。 的首选辅助检测试剂,这些试剂现与BMP-4 Antibody (3H2.3) 打包提供(请参阅下面的订购信息)。

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関連項目

BMP-4抗体(3H2.3)是一种小鼠单克隆IgG2b kappa轻链抗体,可通过应用如蛋白质印迹(WB)、免疫沉淀(IP)和免疫荧光(IF)检测小鼠、大鼠和人类样本中的BMP-4。BMP-4 (3H2.3) 单克隆抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。骨形态发生蛋白(BMPs)包括BMP-4,是转化生长因子-β(TGFβ)超家族的重要成员,在调节骨骼和软骨发育方面发挥着重要作用。BMP-4尤其重要,因为它参与激活Smad信号通路,而Smad信号通路对于调解细胞对BMP信号的反应至关重要。BMP-4与受体结合后,会促进Smad1的磷酸化,使其进入细胞核,从而调节参与骨形成和软骨形成的靶基因的表达。这种调节机制凸显了BMP-4在发育过程和组织稳态中的重要性,以及其在骨相关疾病中的潜在意义。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

BMP-4 抗体 (3H2.3) 参考文献:

  1. 骨生成蛋白-2。转化生长因子-β 超家族的一个新成员,在胚胎形成早期就已表达。  |  Ozkaynak, E., et al. 1992. J Biol Chem. 267: 25220-7. PMID: 1460021
  2. 转化生长因子-β 家族。  |  Massagué, J. 1990. Annu Rev Cell Biol. 6: 597-641. PMID: 2177343
  3. 鉴定从牛骨中纯化的骨诱导蛋白中存在的转化生长因子 beta 家族成员。  |  Celeste, AJ., et al. 1990. Proc Natl Acad Sci U S A. 87: 9843-7. PMID: 2263636
  4. 骨形成的新调节因子:分子克隆和活性。  |  Wozney, JM., et al. 1988. Science. 242: 1528-34. PMID: 3201241
  5. 从细胞外基质中提取BMP-4并分析其与肝素的结合特性。  |  Aykul, S., et al. 2022. Mol Biotechnol. 64: 156-170. PMID: 34550550
  6. 骨关节炎中过度表达的Gremlin-1和BMP-4可驱动成骨细胞和肥厚软骨细胞的骨软骨重塑程序。  |  Pérez-Lozano, ML., et al. 2022. Int J Mol Sci. 23: PMID: 35216203
  7. BMP-4和BMP-7通过调节Notch信号通路,部分抑制前囊下白内障模型中的EMT。  |  Jiang, F., et al. 2023. Invest Ophthalmol Vis Sci. 64: 12. PMID: 37043340
  8. GDF-9、BMP-15、BMP-4和EMMPRIN在卵泡发生和体外受精中的作用。  |  Fountas, S., et al. 2024. J Clin Med. 13: PMID: 38999341
  9. 表达重组 Vgr-1/BMP-6 的肿瘤可诱导体内纤维化和软骨内骨形成。  |  Gitelman, SE., et al. 1994. J Cell Biol. 126: 1595-609. PMID: 8089189
  10. 一种作为 BMP 调节的转录激活因子的人类 Mad 蛋白。  |  Liu, F., et al. 1996. Nature. 381: 620-3. PMID: 8637600
  11. 与受体相关的 Mad 同源物协同作用,成为 TGF-beta 反应的效应因子。  |  Zhang, Y., et al. 1996. Nature. 383: 168-72. PMID: 8774881
  12. 一种新的 TGF-beta 超家族成员对小鼠骨骼肌质量的调节。  |  McPherron, AC., et al. 1997. Nature. 387: 83-90. PMID: 9139826

订购信息

产品名称产品编号规格价格数量收藏夹

BMP-4 抗体 (3H2.3)

sc-12721
200 µg/ml
$316.00

BMP-4 (3H2.3): m-IgG Fc BP-HRP 套装

sc-525459
200 µg Ab; 10 µg BP
$354.00

BMP-4 (3H2.3): m-IgG2b BP-HRP 套装

sc-549563
200 µg Ab; 10 µg BP
$354.00

BMP-4 抗体 (3H2.3) AC

sc-12721 AC
500 µg/ml, 25% agarose
$416.00

BMP-4 抗体 (3H2.3) HRP

sc-12721 HRP
200 µg/ml
$316.00

BMP-4 抗体 (3H2.3) FITC

sc-12721 FITC
200 µg/ml
$330.00

BMP-4 抗体 (3H2.3) PE

sc-12721 PE
200 µg/ml
$343.00

BMP-4 抗体 (3H2.3) Alexa Fluor® 488

sc-12721 AF488
200 µg/ml
$357.00

BMP-4 抗体 (3H2.3) Alexa Fluor® 546

sc-12721 AF546
200 µg/ml
$357.00

BMP-4 抗体 (3H2.3) Alexa Fluor® 594

sc-12721 AF594
200 µg/ml
$357.00

BMP-4 抗体 (3H2.3) Alexa Fluor® 647

sc-12721 AF647
200 µg/ml
$357.00

BMP-4 抗体 (3H2.3) Alexa Fluor® 680

sc-12721 AF680
200 µg/ml
$357.00

BMP-4 抗体 (3H2.3) Alexa Fluor® 790

sc-12721 AF790
200 µg/ml
$357.00

What is the molecular weight of mature BMP-4?

Asked by: AbPolly
The observed weight for the secreted mature form of BMP-4 is approximately 23 kDa.
Answered by: Technical Support 2
Date published: 2017-01-17
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Rated 1 out of 5 by from No band detected in WB for rat WATNo band detected in Western blot (1:100) for rat white adipose tissue.
Date published: 2018-09-12
Rated 1 out of 5 by from Did not work for BMMCs IFTried 1:100 dilution using mouse BMMCs for IF and no signal detected
Date published: 2018-06-21
Rated 5 out of 5 by from Good AntibodyBMP-4 (3H2.3) is well cited, over 20 product citations for recommended applications. Works well transfected lysates as positive control.
Date published: 2017-01-11
Rated 3 out of 5 by from Used on western blots at 1 g/mL with anUsed on western blots at 1 g/mL with an overnight 4 C incubation and a protein-free blocking agent. This antibody cleanly detects 1 ng of recombinant human BMP4. However, in a side-by-side comparison the D-6 antibody (sc-393329) has a stronger signal and lower detection limit.
Date published: 2015-12-01
Rated 4 out of 5 by from Various publishable data in IHC(P) usingVarious publishable data in IHC(P) using mouse temporomandibular joint. -SCBT Publication Review
Date published: 2015-05-19
Rated 3 out of 5 by from Worked with transfected lysate by WBWorked with transfected lysate by WB -SCBT QC
Date published: 2015-05-12
Rated 4 out of 5 by from Publishable data in IF using mouse embryosPublishable data in IF using mouse embryos. -SCBT Publication Review
Date published: 2015-04-15
Rated 4 out of 5 by from Various publishable data in WB using ratVarious publishable data in WB using rat neural stem cell cultures and pulmonary artery smooth muscle . -SCBT Publication Review
Date published: 2015-03-24
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BMP-4 抗体 (3H2.3) is rated 3.2 out of 5 by 10.
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