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PRDM1/Blimp-1 Antibody (C-7) - Western Blotting - Image 308105
Blimp-1 抗体 (C-7): sc-398699. BJAB (A), HeLa (B) 和 Ramos (C) 全细胞裂解液及 HL-60 核提取物 (D) 中 Blimp-1 表达的 Western 印迹分析.
PRDM1/Blimp-1 Antibody (C-7) - Western Blotting - Image 308105
Blimp-1 抗体 (C-7): sc-398699. BJAB (A), HeLa (B) 和 Ramos (C) 全细胞裂解液及 HL-60 核提取物 (D) 中 Blimp-1 表达的 Western 印迹分析.
PRDM1/Blimp-1 Antibody (C-7) - Western Blotting - Image 354458
Blimp-1 抗体 (C-7): sc-398699. Western 印迹分析 Raji 全细胞裂解液中 Blimp-1 的表达.
Blimp-1 抗体 (C-7): sc-398699. BJAB (A), HeLa (B) 和 Ramos (C) 全细胞裂解液及 HL-60 核提取物 (D) 中 Blimp-1 表达的 Western 印迹分析.

PRDM1/Blimp-1 抗体 (C-7): sc-398699

4.3(7)
寫評論提問

说明书
  • PRDM1/Blimp-1抗体(C-7)是小鼠单克隆IgG3 κ, 在1篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的Blimp-1的N-terminus附近的氨基酸21-57之间
  • 推荐用于 mouse, rat 和 human 来源的Blimp-1 WB, IP, IF 和 ELISA检测
  • 可用于ChIP的TransCruz试剂(sc-398699 X, 200 µg/0.1 ml)
  • 看 PRDM1/Blimp-1 (6D3): sc-47732，可查看有标记的 PRDM1/Blimp-1 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 关于如何获取PRDM1/Blimp-1 (C-7): sc-398699的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG3 BP-HRP是PRDM1/Blimp-1 Antibody (C-7) 适用于 WB 应用。 的首选辅助检测试剂，该试剂现与PRDM1/Blimp-1 Antibody (C-7) 搭配使用（请参阅下面的订购信息）。

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関連項目

附加Blimp抗体，包括Blimp-1

PRDM1/Blimp-1 抗体（C-7）是一种 IgG3 κ 小鼠单克隆 Blimp-1 抗体，可通过 WB、IP、IF 和 ELISA 检测小鼠、大鼠和人类来源的 Blimp-1。PRDM1/Blimp-1 抗体（C-7）可以是非偶联的抗 Blimp-1 抗体形式。浆细胞是终末分化的 B 细胞，其发育和分化由 Blimp-1（B 淋巴细胞诱导成熟蛋白，也称为 PRDI-BF1）诱导。Blimp-1 是一种定位于细胞核的转录抑制因子，被认为是终末 B 细胞发育的主调控因子。Blimp-1 单独就足以触发终末 B 细胞分化。Blimp-1 上调 syndecan-1 和 J 链的表达，抑制 IFN-β 基因转录，并与 HDAC 结合将其招募到 DNA 上，从而抑制 c-myc。Blimp-1 在免疫球蛋白分泌的浆细胞的 B 细胞分化晚期以及长寿的骨髓浆细胞中表达。Blimp-1 的表达定义了一个检查点，完全活化的 B 细胞在此之后进入浆细胞阶段，而未成熟和部分活化的细胞则被消除。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

PRDM1/Blimp-1 抗体 (C-7)

sc-398699
200 µg/ml
$316.00

PRDM1/Blimp-1 (C-7): m-IgG3 BP-HRP 套装

sc-550602
200 µg Ab; 40 µg BP
$354.00

PRDM1/Blimp-1 (C-7) 中和勝肽

sc-398699 P
100 µg/0.5 ml
$68.00

PRDM1/Blimp-1 抗体 (C-7) X

sc-398699 X
200 µg/0.1 ml
$316.00

When should the TransCruz reagent sc-398699 X be used instead of the standard form sc-398699?

Asked by: TinTin
We recommend using the TransCruz reagent form, sc-398699 X, when performing ChIP or EMSA assays. This more concetrated form lets you use a smaller volume, which is desired when doing EMSA experiments. The x-form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support 5
Date published: 2017-02-09
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Rated 5 out of 5 by from Great antibodyThe antibody worked very well at 1:1000 dilution. The band was at the expected size and it was perfectly visible.
Date published: 2018-05-08
Rated 1 out of 5 by from Nonspecific in mouse IFNon-specific staining in PFA-fixed, frozen mouse tissue; difficult to tell background versus positive cells (if any)
Date published: 2018-04-03
Rated 4 out of 5 by from Good antibodyWorked well with western blotting at 1:500 dilution.
Date published: 2017-09-27
Rated 5 out of 5 by from strong and clear bandThe antibody can detect Blimp-1 easily in 30 ug protein. The antibody was 1:1000 diluted and the western blot was visualized by HRP,ECL system,the result is strong and clear band.
Date published: 2017-07-22
Rated 5 out of 5 by from Great for Western blotPerfect antibody for Western blot, nice specific bands reliably. Great choice!
Date published: 2017-01-26
Rated 5 out of 5 by from Good staining in IFGood staining in IF
Date published: 2015-05-07
Rated 5 out of 5 by from Very good Western blot data of BlimpVery good Western blot data of Blimp-1 expression in BJAB, HeLa and Ramos whole cell lysates and HL-60 nuclear extract . -SCBT QC
Date published: 2013-04-03
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PRDM1/Blimp-1 抗体 (C-7) is rated 4.3 out of 5 by 7.
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