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PRDM1/Blimp-1 Antibody (C-7) - Western Blotting - Image 308105 — Blimp-1 抗体 (C-7): sc-398699. BJAB (A), HeLa (B) 和 Ramos (C) 全细胞裂解液及 HL-60 核提取物 (D) 中 Blimp-1 表达的 Western 印迹分析.

PRDM1/Blimp-1 Antibody (C-7) - Western Blotting - Image 354458 — Blimp-1 抗体 (C-7): sc-398699. Western 印迹分析 Raji 全细胞裂解液中 Blimp-1 的表达.

Blimp-1 抗体 (C-7): sc-398699. BJAB (A), HeLa (B) 和 Ramos (C) 全细胞裂解液及 HL-60 核提取物 (D) 中 Blimp-1 表达的 Western 印迹分析.

PRDM1/Blimp-1 抗体 (C-7): sc-398699

4.3(7)

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说明书

PRDM1/Blimp-1抗体(C-7)是小鼠单克隆IgG₃ κ, 在1篇文献中引用，规格为200 µg/ml
特异性抗原位于human物种的Blimp-1的N-terminus附近的氨基酸21-57之间
推荐用于 mouse, rat 和 human 来源的Blimp-1 WB, IP, IF 和 ELISA检测
可用于ChIP的TransCruz试剂(sc-398699 X, 200 µg/0.1 ml)
看 PRDM1/Blimp-1 (6D3): sc-47732，可查看有标记的 PRDM1/Blimp-1 抗体，包括AC, HRP, FITC, PE, Alexa Fluor^® 488, 594, 647, 680 和 790
m-IgG₃ BP-HRP是PRDM1/Blimp-1 Antibody (C-7) 适用于 WB 应用。的首选辅助检测试剂，该试剂现与PRDM1/Blimp-1 Antibody (C-7) 搭配使用（请参阅下面的订购信息）。

查看产品引用文献(1)

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PRDM1/Blimp-1 抗体 (C-7) 参考文献:

Blimp-1；免疫球蛋白分泌和向浆细胞的转化。 | Sciammas, R. and Davis, MM. 2005. Curr Top Microbiol Immunol. 290: 201-24. PMID: 16480044
Blimp-1是淋巴恶性肿瘤中的肿瘤抑制基因。 | Hangaishi, A. and Kurokawa, M. 2010. Int J Hematol. 91: 46-53. PMID: 20047096
Blimp-1在树突状细胞中的免疫功能及其与自身免疫性疾病的相关性。 | Kim, SJ. 2015. Immunol Res. 63: 113-20. PMID: 26376898
Blimp-1作为分子开关，可防止Foxp3+RORγt+调节性T细胞的炎症活动。 | Ogawa, C., et al. 2018. Cell Rep. 25: 19-28.e5. PMID: 30282028
Blimp-1有助于调节性B细胞的发展和功能。 | Wang, YH., et al. 2019. Front Immunol. 10: 1909. PMID: 31474988
Blimp-1对于过敏原诱导的哮喘和肺中Th2细胞的发展至关重要。 | He, K., et al. 2020. J Exp Med. 217: PMID: 32399548
多种配体通过EGFR转激活上调Blimp-1，抑制角质形成细胞和鳞状细胞癌中的细胞迁移。 | Lee, H., et al. 2022. Front Pharmacol. 13: 763678. PMID: 35185556
Blimp-1是2级宫颈上皮内瘤变进展的预后指标。 | Saito, M., et al. 2022. J Cancer Res Clin Oncol. 148: 1991-2002. PMID: 35386001
T淋巴细胞中的Blimp-1信号通路对于控制锥虫感染引起的炎症至关重要。 | Benevides, L., et al. 2023. Front Immunol. 14: 1268196. PMID: 37908369
通过Palbociclib/miR-141-3p/STAT4轴调节骨质疏松症的氧化应激：生物信息学和实验验证研究。 | Alvarez-Martinez, M., et al. 2024. Nat Immunol. 25: 886-901. PMID: 38609547

种属	基因名称	基因ID	染色体位置	亚型（mRNA）登录号	蛋白质登录号	OMIM™ 号码
Human	PRDM1	Entrez Gene ID: 639	6q21	XM_006715550, XM_011536062, XM_011536063, XM_011536064, XM_017011187, NM_001198, NM_182907	UniProt ID: O75626	603423
Mouse	Prdm1	Entrez Gene ID: 12142	10 B2	XM_006512502, XM_006512503, XM_006512504, XM_006512505, XM_011243109, NM_007548	UniProt ID: Q60636	N/A

订购信息

产品名称	产品编号	规格	价格	数量	收藏夹
PRDM1/Blimp-1 抗体 (C-7)	sc-398699	200 µg/ml	$322.00
PRDM1/Blimp-1 (C-7): m-IgG₃ BP-HRP 套装	sc-550602	200 µg Ab; 40 µg BP	$361.00
PRDM1/Blimp-1 (C-7) 中和勝肽	sc-398699 P	100 µg/0.5 ml	$69.00
PRDM1/Blimp-1 抗体 (C-7) X	sc-398699 X	200 µg/0.1 ml	$322.00

When should the TransCruz reagent sc-398699 X be used instead of the standard form sc-398699?

Asked by: TinTin

We recommend using the TransCruz reagent form, sc-398699 X, when performing ChIP or EMSA assays. This more concetrated form lets you use a smaller volume, which is desired when doing EMSA experiments. The x-form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.

Answered by: Technical Support 5

Date published: 2017-02-09

Rated 5 out of 5 by lgg86 from Great antibodyThe antibody worked very well at 1:1000 dilution. The band was at the expected size and it was perfectly visible.

Date published: 2018-05-08

Rated 1 out of 5 by rmaywald from Nonspecific in mouse IFNon-specific staining in PFA-fixed, frozen mouse tissue; difficult to tell background versus positive cells (if any)

Date published: 2018-04-03

Rated 4 out of 5 by Tsen from Good antibodyWorked well with western blotting at 1:500 dilution.

Date published: 2017-09-27

Rated 5 out of 5 by cocola1 from strong and clear bandThe antibody can detect Blimp-1 easily in 30 ug protein. The antibody was 1:1000 diluted and the western blot was visualized by HRP,ECL system,the result is strong and clear band.

Date published: 2017-07-22

Rated 5 out of 5 by TinTin from Great for Western blotPerfect antibody for Western blot, nice specific bands reliably. Great choice!

Date published: 2017-01-26

Rated 5 out of 5 by NZ from Good staining in IFGood staining in IF

Date published: 2015-05-07

Rated 5 out of 5 by JT from Very good Western blot data of BlimpVery good Western blot data of Blimp-1 expression in BJAB, HeLa and Ramos whole cell lysates and HL-60 nuclear extract . -SCBT QC

Date published: 2013-04-03

PRDM1/Blimp-1 抗体 (C-7) is rated 4.3 out of 5 by 7.

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PRDM1/Blimp-1 抗体 (C-7): sc-398699

PRDM1/Blimp-1 抗体 (C-7) 参考文献:

PRDM1/Blimp-1 抗体 (C-7) 规格

替代产品名称: PRDM1

产品克隆名称: C-7

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG₃ kappa light chain

氨基酸: 21-57

抗原表位靶标: Blimp-1

抗原表位种属: human

产品反应性/检测: Blimp-1

种属反应性/检测: mouse, rat, human

其它哺乳动物反应性: N/A

其它非哺乳动物反应性: N/A

应用: WB, IP, IF, ELISA

瓶的信息: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 1篇各种科学出版物的引文

PRDM1/Blimp-1 抗体 (C-7)可选的偶联物/形式: P: Blocking Peptide, X: TransCruz Reagent

優選的 PRDM1/Blimp-1 抗體是 PRDM1/Blimp-1 抗體 (6D3): sc-47732.