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β3 Tubulin Antibody (2G10) - Western Blotting - Image 373929
β3 管蛋白抗体 (2G10): sc-80005. PC-12 (A), F9 (B) 和 Neuro-2A (C) 全细胞裂解液中 β3 管蛋白表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.
β3 管蛋白抗体 (2G10): sc-80005. PC-12 (A), F9 (B) 和 Neuro-2A (C) 全细胞裂解液中 β3 管蛋白表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.

β3 Tubulin 抗体 (2G10): sc-80005

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寫評論提問

说明书
  • beta 3 Tubulin 抗体 2G10 是小鼠单克隆 IgG2a κ，β3 Tubulin抗体, 在69篇文献中引用，规格为200 µg/ml
  • 与rat的β3 Tubulin相比，具有与氨基酸436-450的表位图谱
  • beta 3 Tubulin 抗体 (2G10) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat, human 和bovine 来源的 neuronal specific β3 Tubulin
  • 抗beta 3 Tubulin抗体（2G10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取β3 Tubulin (2G10): sc-80005的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 β3 Tubulin 抗体 (2G10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与β3 Tubulin 抗体 (2G10) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Tubulin 抗体包括 α 1 Tubulin, α 3 Tubulin, α 4 Tubulin, α Tubulin, β Tubulin, β 2 Tubulin, β 3 Tubulin, δ Tubulin, ε Tubulin 和 γ Tubulin

β3 Tubulin抗体（2G10）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学检测小鼠、大鼠、人和牛来源的β3 Tubulin蛋白。β3 Tubulin (2G10) 抗体有非结合型和多种结合型可供选择，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。β3微管蛋白在细胞骨架的结构和功能中发挥着关键作用，特别是在神经元中，β3微管蛋白维持着微管的完整性，而微管则支持细胞的形状和运输。β3微管蛋白主要定位于神经元的细胞质中，β3微管蛋白有助于形成对轴突生长和稳定至关重要的微管。β3微管蛋白在神经系统的发育和功能中尤为重要，因为它参与神经元分化、突触连接的形成等过程。β3微管蛋白表达是神经细胞的标志，因此β3微管蛋白是神经生物学研究和神经退行性疾病研究的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

β3 Tubulin 抗体 (2G10) 参考文献:

  1. 有丝分裂中的微管调节：由细胞周期蛋白依赖性激酶Cdk1介导的微管蛋白磷酸化。  |  Fourest-Lieuvin, A., et al. 2006. Mol Biol Cell. 17: 1041-50. PMID: 16371510
  2. 在发育中的鸡视网膜中，引入针对Fgf19的沉默诱导转基因不影响Tbx5和β3-微管蛋白的表达。  |  Okamoto, M., et al. 2008. Dev Growth Differ. 50: 159-68. PMID: 18312426
  3. β3-微管蛋白在人类乳腺癌细胞中通过雌激素受体依赖性途径由雌二醇诱导。  |  Saussede-Aim, J., et al. 2009. Cell Motil Cytoskeleton. 66: 378-88. PMID: 19466750
  4. [应用神经元特异性β3-微管蛋白抗体对人类胎儿犁鼻器结构鼻中隔进行免疫组织化学研究]。  |  Kharlamova, AS., et al. 2011. Arkh Patol. 73: 18-22. PMID: 21695983
  5. β3-微管蛋白是预测原发性乳腺癌对紫杉烷类新辅助化疗敏感性的良好指标。  |  Xiang, Y., et al. 2016. Clin Exp Med. 16: 391-7. PMID: 26088183
  6. β3-微管蛋白对人类恶性黑色素瘤细胞（A375）的微管动力学, 细胞周期调控和微囊自发释放至关重要  |  Altonsy, MO., et al. 2020. Int J Mol Sci. 21: PMID: 32121295
  7. 再生肝-3蛋白磷酸酶（PRL-3）通过与β3-微管蛋白相互作用促进胶质瘤细胞的侵袭性。  |  Ge, Z., et al. 2022. Bioengineered. 13: 4112-4121. PMID: 35098869
  8. 三维系统中的骨髓间充质干细胞可产生更高浓度的胞外囊泡（EV），其复杂性更高，神经元生长特性更强。  |  Jalilian, E., et al. 2022. Stem Cell Res Ther. 13: 425. PMID: 35986305
  9. 基于 CRISPR/Cas9 的癌症诱导神经支配高通量研究分析法。  |  Galappaththi, SL., et al. 2023. Cancers (Basel). 15: PMID: 37046688
  10. 钠-D,L-β-羟基丁酸钠在原代神经元培养物中促进神经再生。  |  Ari, C., et al. 2024. Pharmaceuticals (Basel). 17: PMID: 39338322

订购信息

产品名称产品编号规格价格数量收藏夹

β3 Tubulin 抗体 (2G10)

sc-80005
200 µg/ml
$316.00

β3 Tubulin (2G10): m-IgG Fc BP-HRP 套装

sc-528695
200 µg Ab; 10 µg BP
$354.00

β3 Tubulin (2G10): m-IgGκ BP-HRP 套装

sc-521154
200 µg Ab, 40 µg BP
$354.00

β3 Tubulin (2G10): m-IgG2a BP-HRP 套装

sc-547151
200 µg Ab; 10 µg BP
$354.00

β3 Tubulin 抗体 (2G10) AC

sc-80005 AC
500 µg/ml, 25% agarose
$416.00

β3 Tubulin 抗体 (2G10) HRP

sc-80005 HRP
200 µg/ml
$316.00

β3 Tubulin 抗体 (2G10) FITC

sc-80005 FITC
200 µg/ml
$330.00

β3 Tubulin 抗体 (2G10) PE

sc-80005 PE
200 µg/ml
$343.00

β3 Tubulin 抗体 (2G10) Alexa Fluor® 488

sc-80005 AF488
200 µg/ml
$357.00

β3 Tubulin 抗体 (2G10) Alexa Fluor® 546

sc-80005 AF546
200 µg/ml
$357.00

β3 Tubulin 抗体 (2G10) Alexa Fluor® 594

sc-80005 AF594
200 µg/ml
$357.00

β3 Tubulin 抗体 (2G10) Alexa Fluor® 647

sc-80005 AF647
200 µg/ml
$357.00

β3 Tubulin 抗体 (2G10) Alexa Fluor® 680

sc-80005 AF680
200 µg/ml
$357.00

β3 Tubulin 抗体 (2G10) Alexa Fluor® 790

sc-80005 AF790
200 µg/ml
$357.00

What is the total volume provided of β3 Tubulin (2G10): sc-80005 monoclonal antibody?

Asked by: jenni
Each vial contains 200 µg IgG2a in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.
Answered by: Technical Support 5
Date published: 2017-01-25
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Rated 5 out of 5 by from 功能很出色這支抗體很好用，訊號清楚、背景低，專一性跟穩定性都不錯，操作上也沒什麼問題，實驗結果很穩定!!!!!
Date published: 2025-07-18
Rated 5 out of 5 by from Good immunofluorescenceThis antibody gives excellent immunostaining in differentiated SH-SY5Y cells. Antibody dilution 1:100, incubation overnight at 4°C.
Date published: 2021-12-12
Rated 5 out of 5 by from Great antibody for WB analysisThis antibody gives an extremely good and very specific signal when tested for Western blot analysis in mouse neuroblastoma and brain tissue. Antibody dilution: 1:5.000; incubation for 1h at RT.
Date published: 2021-12-05
Rated 5 out of 5 by from Strong staining with formalin fixed neuronal cellsBought this to try with our iPSC derived neuronal lineages. Antibody produces strong staining on15 minutes formalin fixed samples without antigen retrieval.
Date published: 2021-06-28
Rated 5 out of 5 by from Strong signal !Application: Immunofluorescence in (4% PFA fixed) paraffin embedded specimens of rat colon using heat mediated antigen retrieval with TRIS/EDTA buffer (pH:9, 20min, 92°C) or with citrate buffer (10min, 100°C) and a dilution of 1:50. Conclusion: using the TRIS/EDTA buffer for antigen retrieval, the antibody labels with an excellent resolution all the nerve structures as expected: neurons and nerve fibres of the myenteric plexus and submucosal plexus. Using citrate buffer, the label was identical for distribution although less intense to that observed with the antigen retrieval with TRIS/EDTA buffer.
Date published: 2020-11-24
Rated 5 out of 5 by from ottimo anticorpoOttimo funzionamento. La condizione da noi messa a punto è 1:50 ph6
Date published: 2020-11-19
Rated 5 out of 5 by from Used for measuring the neurite ourgrowthThe expression is clearly different between differentiated and stem cells. used at 1:500 dilution for Immunocytochemistry in NTera2 cells
Date published: 2019-10-05
Rated 5 out of 5 by from works great for IFI used the antibody to stain mouse brain frozen section. it worked great. the concentration was 1:2000, and the sample was fixed in 4%PFA.
Date published: 2019-06-27
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β3 Tubulin 抗体 (2G10) is rated 5.0 out of 5 by 26.
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