Date published: 2025-9-6

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beta Tubulin 抗体 (F-1): sc-166729

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说明书
  • beta Tubulin 抗体 F-1 是小鼠单克隆 IgG3 κ,beta Tubulin抗体, 在91篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于Arabidopsis thaliana物种的β-Tubulin的C-terminus附近的氨基酸422-439之间
  • beta Tubulin 抗体 (F-1) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat, human, Arabidopsis thaliana, Zea mays 和Pisum sativum 来源的 β Tubulin
  • 抗beta Tubulin抗体(F-1)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取beta Tubulin (F-1): sc-166729免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG3 BP-HRP是beta Tubulin Antibody (F-1) 适用于 WB 应用。 的首选辅助检测试剂,该试剂现与beta Tubulin Antibody (F-1) 搭配使用(请参阅下面的订购信息)。

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関連項目

β微管蛋白抗体(F-1)是一种IgG3 κ型小鼠单克隆β微管蛋白抗体(也称为TUBB抗体、TUBB1抗体、I类β微管蛋白抗体、VI类β1微管蛋白抗体或微管结构标记抗体),其能够通过蛋白质印迹、免疫沉淀、免疫荧光和酶联免疫吸附试验检测到来源于小鼠、大鼠、人类、拟南芥、玉米和豌豆的β微管蛋白。β微管蛋白抗体(F-1)既可以以非偶联的抗β微管蛋白抗体形式存在,也可以以与琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)以及多种Alexa Fluor®荧光染料偶联的多种抗β微管蛋白抗体形式存在。微管蛋白是主要的细胞骨架成分,有五种不同的形式,分别命名为α、β、γ、δ和ε微管蛋白。α和β微管蛋白形成异二聚体,这些异二聚体多聚化以形成微管细丝。多种β微管蛋白同工型(β1、β2、β3、β4、β5、β6和β8)已被鉴定出来,并在哺乳动物组织中表达。β1和β4存在于整个细胞质中,β2存在于细胞核和核质中,而β3是神经元特异性的细胞骨架蛋白。γ微管蛋白形成γ体,这是在中心体处核化微管细丝所必需的。δ微管蛋白和ε微管蛋白都与中心体相关。δ微管蛋白是衣藻δ微管蛋白Uni3的同源物,与中心粒相关,而ε微管蛋白则定位于中心粒周围物质。ε微管蛋白表现出细胞周期特异性的定位模式,首先仅与新复制的一对中心体中的较老的一个相关联,随后与两个中心体都相关联。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

beta Tubulin 抗体 (F-1)

sc-166729
200 µg/ml
$316.00

beta Tubulin (F-1): m-IgG3 BP-HRP 套装

sc-551983
200 µg Ab; 40 µg BP
$354.00

beta Tubulin 抗体 (F-1) AC

sc-166729 AC
500 µg/ml, 25% agarose
$416.00

beta Tubulin 抗体 (F-1) HRP

sc-166729 HRP
200 µg/ml
$316.00

beta Tubulin 抗体 (F-1) FITC

sc-166729 FITC
200 µg/ml
$330.00

beta Tubulin 抗体 (F-1) PE

sc-166729 PE
200 µg/ml
$343.00

beta Tubulin 抗体 (F-1) Alexa Fluor® 488

sc-166729 AF488
200 µg/ml
$357.00

beta Tubulin 抗体 (F-1) Alexa Fluor® 546

sc-166729 AF546
200 µg/ml
$357.00

beta Tubulin 抗体 (F-1) Alexa Fluor® 594

sc-166729 AF594
200 µg/ml
$357.00

beta Tubulin 抗体 (F-1) Alexa Fluor® 647

sc-166729 AF647
200 µg/ml
$357.00

beta Tubulin 抗体 (F-1) Alexa Fluor® 680

sc-166729 AF680
200 µg/ml
$357.00

beta Tubulin 抗体 (F-1) Alexa Fluor® 790

sc-166729 AF790
200 µg/ml
$357.00

beta Tubulin (F-1) 中和勝肽

sc-166729 P
100 µg/0.5 ml
$68.00

Hi, I am working on Mimulus, I would like to use a protein antibody as a reference for samples , I am wondering how to find out if the arabidopsis version works for mimulus plants? Thanks!

Asked by: bqing
Thank you for your question. You could get in touch with our technical service team to arrange a free sample of this antibody.
Answered by: Technical Support Europe
Date published: 2020-10-14

What application is the blocking peptide sc-166729 P appropriate for?

Asked by: jenni
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support 3
Date published: 2023-11-14
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Rated 3 out of 5 by from Buon funzionamentoHo comprato questo anticorpo un mese fa circa ed è stato testato 1:1000 su astrociti. Le bande sono intorno a 55kDa, ma il segnale è debole
Date published: 2021-03-17
Rated 5 out of 5 by from This antibody works beautifully!I ordered a free sample about a month ago. We tried it with Arabidopsis protein extract. It recognize Arabidopsis b-tubulin at 1:1000 dilution. There was no background signal.
Date published: 2018-03-08
Rated 3 out of 5 by from Nice Tubulin band, but with nonspecific bandsRan this antibody in Huh7 cells. Band at 55 kDa was nice and clear, but had two faint nonspecific bands at ~25 and 70 kDa.
Date published: 2017-12-15
Rated 4 out of 5 by from greatI like this antibody as it has strong band binding and no background.
Date published: 2017-10-13
Rated 5 out of 5 by from Highly specific antibody in tobacco + arabidopsisClear and highly specific band of medium strength in total leave extract from both tobacco (N. benthamiana) and Arabidopsis. The detection was mediated using a secondary antibody coupled to a near infra red fluorescent dye by LICOR.
Date published: 2017-10-10
Rated 4 out of 5 by from Great!It worked well for Hela cell samples and HEK293 cells
Date published: 2017-10-03
Rated 4 out of 5 by from Good qualityThe antibody gives good result for western blotting assays (dilution 1/500), good signal strength. We obtained two bands when tested tissue samples (mice liver). However, it was clear which band was the specific one.
Date published: 2017-09-12
Rated 5 out of 5 by from Great Antibody!I'm glad to use it and get good results with it in IF and WB.
Date published: 2017-04-24
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beta Tubulin 抗体 (F-1) is rated 4.5 out of 5 by 13.
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