Date published: 2025-9-5
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β-Gal Antibody (B-12) - Western Blotting - Image 354869
β-Gal (B-12): sc-377257. SH-SY5Y (A), HeLa (B), A549 (C), Jurkat (D), MCF7 (E) 和 MIA PaCa-2 (F) 全细胞裂解液中 β-Gal 表达的 Western 印迹分析.
β-Gal (B-12): sc-377257. SH-SY5Y (A), HeLa (B), A549 (C), Jurkat (D), MCF7 (E) 和 MIA PaCa-2 (F) 全细胞裂解液中 β-Gal 表达的 Western 印迹分析.

β-Gal 抗体 (B-12): sc-377257

3.9(11)
寫評論提問

说明书
  • beta-Gal 抗体 B-12 是小鼠单克隆 IgG2a κ，β-Gal抗体, 在13篇文献中引用，规格为200 µg/ml
  • 免疫human物种的β-Gal的氨基酸496-575
  • beta-Gal 抗体 (B-12) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 β-Gal
  • 抗beta-Gal抗体（B-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取β-Gal (B-12): sc-377257的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 β-Gal 抗体 (B-12) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与β-Gal 抗体 (B-12) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Galactosidase 抗体包括 β-Galactosidase

β-Gal抗体（B-12）是一种IgG2a κ小鼠单克隆β-Gal抗体（也称为GLB1抗体、BGAL抗体、Galactosidase Beta 1抗体、Elastin Receptor 1 67kDa抗体、Elastin Binding Protein抗体、Acid Beta-Galactosidase抗体、Beta-Galactosidase抗体、Lactase抗体、ELNR1抗体、EBP抗体、Elastin Receptor 1 (67kD)抗体、MPS4B抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的β-Gal蛋白。β-Gal抗体（B-12）既有非偶联形式的抗β-Gal抗体，也有多种偶联形式的抗β-Gal抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。人类β-半乳糖苷酶基因，也称为LacZ基因，位于3p21.33染色体上，编码一个由677个氨基酸组成的蛋白质，其最佳功能pH范围为6至8。具有催化活性的β-半乳糖苷酶（β-Gal）是由四个相同的亚基组成的四聚体，每个亚基都有一个活性位点，可独立催化末端半乳糖的裂解。一价阳离子对酶促反应有刺激作用，这可能涉及一个半乳糖基-酶复合中间体。β-Gals广泛存在于动物、微生物和植物中。LacZ基因被广泛用作报告基因，能够产生多种有色或荧光化合物，这些化合物可从适当的底物中产生，如Xgal，可产生蓝色。因此，LacZ被作为标记物整合到许多质粒载体中。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

β-Gal 抗体 (B-12) 参考文献:

  1. 大肠杆菌 beta-半乳糖苷酶单克隆抗体及其在检测和纯化重组表达产物中的应用。  |  Dráber, P., et al. 1992. Hybridoma. 11: 385-90. PMID: 1500073
  2. 人类皮肤成纤维细胞衰老相关β-半乳糖苷酶（SA-β-Gal）的表达，晚期糖基化终产物和富含岩藻糖或鼠李糖的多糖的影响。  |  Ravelojaona, V., et al. 2009. Arch Gerontol Geriatr. 48: 151-4. PMID: 18207583
  3. 凝结芽孢杆菌RCS3的β-半乳糖苷酶同工酶在乳糖水解中的应用。  |  Batra, N., et al. 2011. Int J Biol Macromol. 49: 879-84. PMID: 21855568
  4. 黑色素瘤肿瘤细胞中的β-Gal基因表达MRI报告。设计、合成、体外和体内测试一种Gd（III）探针，该探针在β-Gal酶激活后形成高弛豫性、类黑色素结构。  |  Arena, F., et al. 2011. Bioconjug Chem. 22: 2625-35. PMID: 22035020
  5. 小鼠早期胚胎中鼠金属硫蛋白-大肠杆菌β-半乳糖苷酶融合基因（MT-β gal）的表达。  |  Stevens, ME., et al. 1989. Exp Cell Res. 183: 319-25. PMID: 2504613
  6. β-半乳糖苷酶 mRNA 的交替剪接会产生经典的溶酶体酶和β-半乳糖苷酶相关蛋白。  |  Morreau, H., et al. 1989. J Biol Chem. 264: 20655-63. PMID: 2511208
  7. 开发一种高通量β-半乳糖苷酶中和检测法，用于定量检测人类样本中的单纯疱疹病毒中和抗体。  |  Baccari, A., et al. 2016. Vaccine. 34: 3901-6. PMID: 27265458
  8. 人 beta-半乳糖苷酶 cDNA 的克隆, 测序和表达。  |  Oshima, A., et al. 1988. Biochem Biophys Res Commun. 157: 238-44. PMID: 3143362
  9. 从碳水化合物衍生的酮合成"全顺式"三羟基哌啶：新型β-半乳糖苷酶和半乳糖苷酶抑制剂的设计提示。  |  Davighi, MG., et al. 2020. Molecules. 25: PMID: 33023214
  10. 预测非生物胁迫下β-GAL基因在豆类中的作用，以及β-GAL基因家族成员的全基因组表征。  |  Buttanri, A., et al. 2024. Protoplasma.. PMID: 39441340
  11. 通过荧光原位杂交将人类 beta-半乳糖苷酶-A基因定位到 3p21.33。  |  Takano, T. and Yamanouchi, Y. 1993. Hum Genet. 92: 403-4. PMID: 7693577
  12. 针对大肠杆菌 beta-半乳糖苷酶的新型单克隆抗体及其在检测重组表达产物方面的多功能性。  |  Slavícková, A., et al. 1992. Folia Biol (Praha). 38: 350-7. PMID: 1284241

订购信息

产品名称产品编号规格价格数量收藏夹

β-Gal 抗体 (B-12)

sc-377257
200 µg/ml
$316.00

β-Gal (B-12): m-IgGκ BP-HRP 套装

sc-523251
200 µg Ab, 40 µg BP
$354.00

β-Gal (B-12): m-IgG2a BP-HRP 套装

sc-546151
200 µg Ab; 10 µg BP
$354.00

β-Gal 抗体 (B-12) AC

sc-377257 AC
500 µg/ml, 25% agarose
$416.00

β-Gal 抗体 (B-12) HRP

sc-377257 HRP
200 µg/ml
$316.00

β-Gal 抗体 (B-12) FITC

sc-377257 FITC
200 µg/ml
$330.00

β-Gal 抗体 (B-12) PE

sc-377257 PE
200 µg/ml
$343.00

β-Gal 抗体 (B-12) Alexa Fluor® 488

sc-377257 AF488
200 µg/ml
$357.00

β-Gal 抗体 (B-12) Alexa Fluor® 546

sc-377257 AF546
200 µg/ml
$357.00

β-Gal 抗体 (B-12) Alexa Fluor® 594

sc-377257 AF594
200 µg/ml
$357.00

β-Gal 抗体 (B-12) Alexa Fluor® 647

sc-377257 AF647
200 µg/ml
$357.00

β-Gal 抗体 (B-12) Alexa Fluor® 680

sc-377257 AF680
200 µg/ml
$357.00

β-Gal 抗体 (B-12) Alexa Fluor® 790

sc-377257 AF790
200 µg/ml
$357.00

How well does it work in IF of glioma?

Asked by: anglelababay
Thank you for your question. Glioma cells in particular have not been tested, but Anti-β-Gal Antibody (B-12): sc-377257 is recommended for detection of β-Gal of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. If the Glioma cells you are using stem from one of these species, the antibody will be able to detect its target protein in them.
Answered by: Tech Support Europe
Date published: 2022-03-29

Has it been used in fixed IHC?

Asked by: JMTR210
This antibody has been validated for IHC and IHC (P)
Answered by: Tech Sales
Date published: 2021-06-04

Is there a blocking peptide for this antibody?

Asked by: AbPolly
Thank you for your question. Our B-Gal sc-377257 was raised against a recombinant protein and not a short peptide sequence, so it is not possible to design a blocking peptide for it.
Answered by: Technical Service
Date published: 2017-02-03
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Rated 4 out of 5 by from Good detection in human immortalized fibroblastsI tested the antibody at 1:1000 dilution in T-TBS 0.05%, using an overnight incubation at 4°C, and I obtained good signal detection in protein extracts from human immortalized fibroblasts.
Date published: 2024-12-10
Rated 5 out of 5 by from This antibody works very wellWe got this antibody. It is one of the best anti-TRIM5a I ever used. I could easily detect other primate TRIM5a proteins as well.
Date published: 2020-05-27
Rated 1 out of 5 by from Didn't work!Although worked perfectly in IF for lacZ reporter in transgenic mice it was not especific enough to be competent to give more than 1 star because the control negative had the same pattern as the sample.
Date published: 2018-10-25
Rated 5 out of 5 by from Great for IHCI've tried this in transgenic mice samples with a lacZ reporter and it gave fantastic results. Antigen retrieval with sodium citrate buffer pH 6.0, Dilution 1:100, incubated 2h RT Used with Novolink polymer kit
Date published: 2017-12-18
Rated 1 out of 5 by from Bgal thumbs down for western blotEven at 1:100 in senescence induced cell line lysate did not work via western blot at 1:100. One hour transfer PVDF XCell Blot module 30ug protein loaded, Gapdh perfect.
Date published: 2017-11-30
Rated 4 out of 5 by from good for western blottingI got a sample antibody and it is working fine with no background. A good antibody.
Date published: 2017-09-07
Rated 5 out of 5 by from Good antibody for beta-GalRobust signal seen in Western blot for multiple cell lines, and great IHC(P) staining. Great antibody for mouse, rat, and human detection.
Date published: 2017-01-30
Rated 4 out of 5 by from beta-Gal feel okThe results was well; paraffin-embedded human liver tissue
Date published: 2016-11-22
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β-Gal 抗体 (B-12) is rated 3.9 out of 5 by 11.
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