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Bcl-6 Antibody (D-8) - Western Blotting - Image 399001
Bcl-6 抗体 (D-8): sc-7388. BJAB (A), NAMALWA (B) 和 Ramos (C) 全细胞裂解液中 Bcl-6 表达的 Western 印迹分析. 所用检测试剂: m-IgG3 BP-HRP: sc-533670.
Bcl-6 抗体 (D-8): sc-7388. BJAB (A), NAMALWA (B) 和 Ramos (C) 全细胞裂解液中 Bcl-6 表达的 Western 印迹分析. 所用检测试剂: m-IgG3 BP-HRP: sc-533670.

Bcl-6 抗体 (D-8): sc-7388

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说明书
  • Bcl-6 抗体 D-8 是小鼠单克隆 IgG3 κ，Bcl-6抗体, 在60篇文献中引用，规格为200 µg/ml
  • 免疫human来源Bcl-6位于N-terminus 的3-484氨基酸
  • 推荐用于 mouse, rat 和 human 来源的Bcl-6 WB, IP, IF 和 IHC(P)检测
  • 另提供琼脂糖偶联抗体，规格为琼脂糖装，可用于IP实验；HRP 偶联抗体用于WB, IHC(P)和ELISA实验；以及藻红蛋白、FITC、Alexa Fluor® 488 或Alexa Fluor® 647 偶联抗体，规格为用于IF、IHC(P)和FCM实验
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-7388 X, 200 µg/0.1 ml)
  • 关于如何获取Bcl-6 (D-8): sc-7388的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG3 BP-HRP 是Bcl-6 抗体 (D-8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与Bcl-6 抗体 (D-8) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 Bcl-6 抗体包括 Bcl-6b

Bcl-6 抗体（D-8）是一种小鼠单克隆 IgG3 kappa 轻链抗体，旨在使用免疫印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学（IHCP）技术检测小鼠、大鼠和人类中的 Bcl-6 蛋白（也称为 BCL6 转录抑制因子）。Bcl-6 单克隆抗体（D-8）靶向人 Bcl-6 蛋白 N 端 3-484 氨基酸的表位，确保精确可靠的结合。Bcl-6 (D-8) 抗体识别一种关键的转录抑制因子，该因子参与调节生发中心发育以及 T 辅助细胞向 Th1 和 Th2 亚群的分化，而这是适应性免疫反应中的关键过程。Bcl-6 (D-8) 单克隆抗体可检测出一种关键调节因子，该因子可负调控 NFkB 表达，从而抑制 NFkB 介导的细胞功能，这些功能对于维持细胞稳态和防止过度炎症反应至关重要。HDAC 和 SIR2 依赖性乙酰化可通过阻止组蛋白去乙酰化酶复合物的招募来调节 Bcl-6 的活性，从而突显出细胞内复杂的调节机制。在非霍奇金淋巴瘤中经常观察到Bcl-6的失调，这凸显了其在肿瘤发生中的关键作用，以及作为治疗干预的宝贵靶标的重要性。Bcl-6 (D-8) 单克隆抗体有非结合型和结合型两种形式，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®结合物，为各种实验应用提供了多种选择。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Bcl-6 抗体 (D-8) 参考文献:

  1. 档案标本中生殖中心 B 细胞淋巴瘤的检测：扁桃体弥漫性大 B 细胞淋巴瘤中 Bcl-6 蛋白表达的关键评估。  |  Ree, HJ., et al. 2003. Hum Pathol. 34: 610-6. PMID: 12827616
  2. BCL-6 在乳腺癌中表达，可阻止乳腺上皮分化。  |  Logarajah, S., et al. 2003. Oncogene. 22: 5572-8. PMID: 12944904
  3. 浸润性乳腺癌中 B 细胞淋巴瘤基因 6（BCL-6）的蛋白表达与细胞周期蛋白 D1 和缺氧诱导因子-1alpha（HIF-1alpha）有关。  |  Bos, R., et al. 2003. Oncogene. 22: 8948-51. PMID: 14654791
  4. 肺淋巴增生性疾病中的 BCL-6 基因突变：HIV 相关淋巴性间质性肺炎中异常免疫反应的证明。  |  Kurosu, K., et al. 2004. J Immunol. 172: 7116-22. PMID: 15153535
  5. 非霍奇金淋巴瘤的分子发病机制：Bcl-6 的作用。  |  Pasqualucci, L., et al. 2003. Leuk Lymphoma. 44 Suppl 3: S5-12. PMID: 15202519
  6. Bcl-6对prdm1的直接抑制抑制了浆细胞分化。  |  Tunyaplin, C., et al. 2004. J Immunol. 173: 1158-65. PMID: 15240705
  7. IL-21 是 Blimp-1 和 Bcl-6 的新型诱导剂，可调节 B 细胞分化和浆细胞生成。  |  Ozaki, K., et al. 2004. J Immunol. 173: 5361-71. PMID: 15494482
  8. BCL-6 负向调节 NF-kappaB1 p105/p50 亚基的表达。  |  Li, Z., et al. 2005. J Immunol. 174: 205-14. PMID: 15611242
  9. 抑制白细胞介素-4反应型启动子需要BCL-6的协同功能。  |  Harris, MB., et al. 2005. J Biol Chem. 280: 13114-21. PMID: 15659391

订购信息

产品名称产品编号规格价格数量收藏夹

Bcl-6 抗体 (D-8)

sc-7388
200 µg/ml
$316.00

Bcl-6 (D-8): m-IgG3 BP-HRP 套装

sc-550543
200 µg Ab; 40 µg BP
$354.00

Bcl-6 抗体 (D-8) AC

sc-7388 AC
500 µg/ml, 25% agarose
$416.00

Bcl-6 抗体 (D-8) Alexa Fluor® 488

sc-7388 AF488
200 µg/ml
$357.00

Bcl-6 抗体 (D-8) Alexa Fluor® 647

sc-7388 AF647
200 µg/ml
$357.00

Bcl-6 抗体 (D-8) FITC

sc-7388 FITC
200 µg/ml
$330.00

Bcl-6 抗体 (D-8) HRP

sc-7388 HRP
200 µg/ml
$316.00

Bcl-6 抗体 (D-8) PE

sc-7388 PE
200 µg/ml
$343.00

Bcl-6 抗体 (D-8) X

sc-7388 X
200 µg/0.1 ml
$316.00

Hi, I understand that you have discontinued Bcl-6 Antibody clone (N-3) and replaced it with sc-7388. Is the D-8 clone (sc-7388) suitable for cHIP qPCR? Thats what the N-3 widely used for. Can I have a trial size to test? Thank you. -Isa

Asked by: Anonymous
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801ext.1, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2021-11-23

Mi pregunta es que clon es el del producto sc-7388 / que tipo de buffer de recuperación antigenica recomiendan?

Asked by: MoniLo
Thank you for your question. Yes, we recommend antigen retrieval for this antibody, and I would suggest sodium citrate with heat. You can find the complete recommended protocol here: https://www.scbt.com/resources/protocols/immunoperoxidase-staining
Answered by: Tech Service
Date published: 2019-12-06

When should the TransCruz reagent sc-7388 X be used instead of the standard form sc-7388?

Asked by: jenni
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support 10
Date published: 2017-01-09
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Rated 5 out of 5 by from 使いやすい抗体WBと免疫蛍光染色で使用し、どちらも満足できる結果がえられました。複数のアプリケーションで使用でき、とても使いやすい抗体だと思います。
Date published: 2020-10-16
Rated 2 out of 5 by from Not as good as former antibodyWasn't that great, much weaker signal than with Santa Cruz rabbit antibody that we used to have (on DLBCL cells that are expressing high levels of Bcl-6), we need to test more Abs
Date published: 2017-11-06
Rated 3 out of 5 by from 结果不是太好几天前用之前活动申请的小样做了一下，但是没有出现目的条带。样品是小鼠腹腔巨噬细胞，抗体稀释比为1：500，之后1:200重新做也没有显出目的条带。
Date published: 2017-07-18
Rated 5 out of 5 by from Works very well in WBI used this antibody at 1:500 in multiple myeloma cells. I got very specific band in western blot.
Date published: 2017-01-26
Rated 5 out of 5 by from Strong protein detectionWorks great for Bcl-6 detection in Western blots and immunostains. It will provide you with a strong clear signal. This antibody has been published with more than 15 times.
Date published: 2017-01-16
Rated 5 out of 5 by from Outstanding!!Very pleased with all applications including western, IF and IHC
Date published: 2017-01-06
Rated 4 out of 5 by from Various publishable data in WB using human CB33Various publishable data in WB using human CB33, RD, Ramos, Val, GC B cells, transfected 293T cells, Daudi, P3HR1, or DG75 or ER/EB2-5 cell lines. -SCBT Publication Review
Date published: 2015-05-16
Rated 4 out of 5 by from Publishable data in FCM using mouse thymocytesPublishable data in FCM using mouse thymocytes. -SCBT Publication Review
Date published: 2015-04-26
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Bcl-6 抗体 (D-8) is rated 4.2 out of 5 by 13.
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