Date published: 2025-9-6
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BAT1/DDX39 Antibody (H-6) - Western Blotting - Image 360072
BAT1/DDX39 抗体 (H-6): sc-271395. Hep G2 (A), A-431 (B), C3H/10T1/2 (C), 3T3-L1 (D), C6 (E) 和 A-10 (F) 全细胞裂解液中 BAT1/DDX39 表达的 Western 印迹分析.
BAT1/DDX39 抗体 (H-6): sc-271395. Hep G2 (A), A-431 (B), C3H/10T1/2 (C), 3T3-L1 (D), C6 (E) 和 A-10 (F) 全细胞裂解液中 BAT1/DDX39 表达的 Western 印迹分析.

BAT1/DDX39 抗体 (H-6): sc-271395

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说明书
  • BAT1/DDX39 抗体 H-6 是小鼠单克隆 IgG2a κ，BAT1/DDX39抗体, 在5篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列231-428位于human物种的BAT1的C-terminus
  • BAT1/DDX39 抗体 (H-6) 推荐用于WB, IP, IF, IHC(P) 和 ELISA ，检测 mouse, rat, human 和 来源的BAT1 and DDX39; 也和以下物种反应，包括： 和 equine, canine, bovine, porcine and avian
  • 抗BAT1/DDX39抗体（H-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-271395 X, 200 µg/0.1 ml)
  • 关于如何获取BAT1/DDX39 (H-6): sc-271395的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是BAT1/DDX39 Antibody (H-6) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与BAT1/DDX39 Antibody (H-6) 打包提供（请参阅下面的订购信息）。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

附加DDX抗体，包括DDX1, DDX11, DDX15, DDX16, DDX17, DDX21, DDX25, DDX27, DDX29, DDX3, DDX30, DDX32, DDX33, DDX34, DDX36和DDX38

BAT1/DDX39抗体（H-6）是一种IgG2a κ小鼠单克隆BAT1/DDX39抗体（也称为BAT1/DDX39抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的BAT1/DDX39蛋白。BAT1/DDX39抗体（H-6）既可作为非共轭的抗BAT1/DDX39抗体形式提供，也可作为多种共轭形式的抗BAT1/DDX39抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。BAT1（剪接体RNA解旋酶BAT1），也称为DEAD-box蛋白UAP56（56 kDa U2AF65相关蛋白）、HLA-B相关转录本-1或ATP依赖性RNA解旋酶p47，是DEAD-box解旋酶中DECD亚家族的成员。BAT1对mRNA剪接和核输出很重要，它与mRNA输出因子ALY相互作用，并作为剪接因子发挥作用，介导剪接体组装的第一个ATP依赖性步骤。BAT1与转录延长因子THO蛋白结合，形成TREX复合物，并与剪接机制相互作用，形成外显子连接复合物。由于选择性剪接事件，BAT1以两种同工型存在。与BAT1一样，DDX39（DEAD box蛋白39）也是DEAD-box解旋酶家族的成员。DDX39定位于细胞核，并在肺、脑、肾、脾、胸腺和唾液腺中表达。DDX39的功能与BAT1相似，参与pre-mRNA剪接和mRNA从细胞核的输出。DDX39在肺鳞状细胞癌中的表达上调，提示DDX39在肿瘤发生中的作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

BAT1/DDX39 抗体 (H-6) 参考文献:

  1. 细胞剪接因子RAF-2p48/NPI-5/BAT1/UAP56与流感病毒核蛋白相互作用，并增强病毒RNA的合成。  |  Momose, F., et al. 2001. J Virol. 75: 1899-908. PMID: 11160689
  2. UAP56 与 Aly 之间的直接相互作用将前核糖核酸剪接和核糖核酸输出联系起来。  |  Luo, ML., et al. 2001. Nature. 413: 644-7. PMID: 11675789
  3. BAT1 基因启动子中 -22 和 -348 位的多态性会影响转录和核因子的结合。  |  Price, P., et al. 2004. Hum Mol Genet. 13: 967-74. PMID: 15028669
  4. 人类 ATP 依赖性剪接和输出因子 UAP56 的晶体结构。  |  Shi, H., et al. 2004. Proc Natl Acad Sci U S A. 101: 17628-33. PMID: 15585580
  5. BRCA2 相互作用的同源物 Dss1p 和 Uap56p 在裂殖酵母中连接 Mlo3p 和 Rae1p 以输出 mRNA。  |  Thakurta, AG., et al. 2005. EMBO J. 24: 2512-23. PMID: 15990877
  6. BAT1是一种假定的抗炎基因，与慢性恰加斯病心肌病有关。  |  Ramasawmy, R., et al. 2006. J Infect Dis. 193: 1394-9. PMID: 16619187
  7. siRNA 减少哺乳动物 RNA 螺旋酶 UAP56 和 URH49 的表达后 poly(A)+ mRNA 的核定域。  |  Kapadia, F., et al. 2006. Gene. 384: 37-44. PMID: 16949217
  8. DDX39 在肺鳞癌中上调，具有 RNA 螺旋酶活性，能促进癌细胞生长。  |  Sugiura, T., et al. 2007. Cancer Biol Ther. 6: 957-64. PMID: 17548965
  9. DDX39作为透明细胞肾细胞癌患者临床预后和免疫检查点治疗疗效的预测因子。  |  Bao, Y., et al. 2021. Int J Biol Sci. 17: 3158-3172. PMID: 34421357
  10. CASP1基因多态性与BAT1-NFKBIL-LTA-CASP1基因-基因相互作用与冠状动脉支架置入术后再狭窄有关。  |  Vargas-Alarcón, G., et al. 2022. Biomolecules. 12: PMID: 35740890
  11. Ddx39在胚胎干细胞分化与端粒长度调节中的新作用。  |  Nai, S., et al. 2024. Cell Death Differ. 31: 1534-1544. PMID: 39107495
  12. U2AF65 招募 U2 snRNP-分支点相互作用所需的新型人类 DEAD box 蛋白。  |  Fleckner, J., et al. 1997. Genes Dev. 11: 1864-72. PMID: 9242493

订购信息

产品名称产品编号规格价格数量收藏夹

BAT1/DDX39 抗体 (H-6)

sc-271395
200 µg/ml
$316.00

BAT1/DDX39 (H-6): m-IgG Fc BP-HRP 套装

sc-529174
200 µg Ab; 10 µg BP
$354.00

BAT1/DDX39 (H-6): m-IgGκ BP-HRP 套装

sc-521931
200 µg Ab, 40 µg BP
$354.00

BAT1/DDX39 (H-6): m-IgG2a BP-HRP 套装

sc-547273
200 µg Ab; 10 µg BP
$354.00

BAT1/DDX39 抗体 (H-6) X

sc-271395 X
200 µg/0.1 ml
$316.00

BAT1/DDX39 抗体 (H-6) AC

sc-271395 AC
500 µg/ml, 25% agarose
$416.00

BAT1/DDX39 抗体 (H-6) HRP

sc-271395 HRP
200 µg/ml
$316.00

BAT1/DDX39 抗体 (H-6) FITC

sc-271395 FITC
200 µg/ml
$330.00

BAT1/DDX39 抗体 (H-6) PE

sc-271395 PE
200 µg/ml
$343.00

BAT1/DDX39 抗体 (H-6) Alexa Fluor® 488

sc-271395 AF488
200 µg/ml
$357.00

BAT1/DDX39 抗体 (H-6) Alexa Fluor® 546

sc-271395 AF546
200 µg/ml
$357.00

BAT1/DDX39 抗体 (H-6) Alexa Fluor® 594

sc-271395 AF594
200 µg/ml
$357.00

BAT1/DDX39 抗体 (H-6) Alexa Fluor® 647

sc-271395 AF647
200 µg/ml
$357.00

BAT1/DDX39 抗体 (H-6) Alexa Fluor® 680

sc-271395 AF680
200 µg/ml
$357.00

BAT1/DDX39 抗体 (H-6) Alexa Fluor® 790

sc-271395 AF790
200 µg/ml
$357.00

Hello, could you provide me an exact sequence of immunogen? Does this antibody recognise both paralogs DDX39A and DDX39B?

Asked by: Ktutak
Thank you for your question. This antibody, sc-271395, was raised against amino acids 231-428 mapping at the C-terminus of BAT1 of human origin (accession#Q13838). The exact epitope has not been mapped. It detects both DDX39A and DDX39B.
Answered by: Tech Service
Date published: 2019-08-30

Are there any issues with using sc-271395: BAT1/DDX39 (H-6) mouse monoclonal primary antibody on mouse samples?

Asked by: Cweed
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-271395 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 5 out of 5 by from high specificityThe antibody can detect the DDX39 in Hela cells using Western blotting(1:400).
Date published: 2017-12-26
Rated 5 out of 5 by from goodI used this antibody as a nuclear marker when I do the cytoplasmic and nuclear fraction experiment and it works quite good.
Date published: 2017-09-13
Rated 4 out of 5 by from High specificityThis antibody can detect my protein well using hypersensitivity ECL.
Date published: 2016-10-27
Rated 5 out of 5 by from Good Western Blot data of BAT1 expression in nonGood Western Blot data of BAT1 expression in non-transfected 293T, human BAT1 transfected 293T and HeLa whole cell lysates. -SCBT QC
Date published: 2015-04-25
Rated 5 out of 5 by from Great Western Blot data of BAT1/DDX39 expressionGreat Western Blot data of BAT1/DDX39 expression in Hs 181 Tes whole cell lysate and K-562, Jurkat and HeLa nuclear extracts. -SCBT QC
Date published: 2013-07-12
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BAT1/DDX39抗体 (H-6) is rated 4.8 out of 5 by 5.
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