Date published: 2025-9-11

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Baculovirus gp64 抗体 (AcV1): sc-65498

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说明书
  • Baculovirus gp64 抗体 AcV1 是小鼠单克隆 IgG2a κ,Baculovirus gp64抗体, 在16篇文献中引用,规格为200 µg/ml
  • 针对AcNPV extracellular nonoccluded virus (NOV)
  • Baculovirus gp64 抗体 (AcV1) 推荐用于WBIPFCM,检测AcNPV 来源的 baculovirus envelope protein gp64
  • 抗Baculovirus gp64抗体(AcV1)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Baculovirus gp64 (AcV1): sc-65498免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgGκ BP-HRP是Baculovirus gp64 Antibody (AcV1) 适用于 WB 应用。 的首选辅助检测试剂,这些试剂现与Baculovirus gp64 Antibody (AcV1) 打包提供(请参阅下面的订购信息)。

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関連項目

杆状病毒 gp64 抗体(AcV1)是一种小鼠单克隆 IgG2a kappa 轻链抗体,设计用于检测杆状病毒包膜糖蛋白 gp64,又称同源三聚杆状病毒膜糖蛋白 gp64 或主要包膜糖蛋白 GP64、 利用免疫印迹(WB)、免疫沉淀(IP)和流式细胞术(FCM)应用研究了来自加州古树多核多角体病毒(AcNPV)的GP64。GP64 是参与病毒生命周期的关键蛋白,主要负责介导病毒包膜与宿主细胞膜的融合,这对病毒的进入和感染至关重要。GP64 位于受感染细胞和出芽病毒的表面,有助于病毒在宿主体内传播的附着和融合过程。从结构上看,GP64 是一种三聚磷酸糖蛋白,具有多个连接的糖基化位点和疏水末端,可将 GP64 牢固固定在病毒膜上,确保其稳定性和功能性。翻译后修饰,尤其是糖基化,对于 GP64 的正确折叠和免疫识别、增强病毒的感染性以及与宿主细胞受体的相互作用至关重要。GP64 与各种宿主蛋白相互作用,协调病毒的进入过程,因此 GP64 是研究杆状病毒与宿主相互作用和开发抗病毒策略的关键目标。抗 gp64 抗体 (AcV1) 既有非共轭形式,也有多种共轭形式,包括辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和各种 Alexa Fluor® 共轭物,为研究杆状病毒感染机制、基因传递系统和蛋白质表达研究提供了多功能工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Baculovirus gp64 抗体 (AcV1) 参考文献:

  1. 杆状病毒 GP64 介导的膜融合的离散阶段。  |  Kingsley, DH., et al. 1999. Mol Biol Cell. 10: 4191-200. PMID: 10588652
  2. 杆状病毒 gp64 包膜糖蛋白足以介导 pH 依赖性膜融合。  |  Blissard, GW. and Wenz, JR. 1992. J Virol. 66: 6829-35. PMID: 1404622
  3. 杆状病毒 GP64 蛋白介导了缺乏同源跨膜糖蛋白的人类呼吸道合胞病毒的高度稳定感染性。  |  Oomens, AG. and Wertz, GW. 2004. J Virol. 78: 124-35. PMID: 14671094
  4. 杆状病毒 gp64 基因的表达:IE1 对早期转录和反式激活的调控序列分析。  |  Blissard, GW. and Rohrmann, GF. 1991. J Virol. 65: 5820-7. PMID: 1920618
  5. 杆状病毒 GP64 包膜融合蛋白:合成, 寡聚和加工。  |  Oomens, AG., et al. 1995. Virology. 209: 592-603. PMID: 7778291
  6. 杆状病毒 gp64 包膜融合蛋白基因中重叠的 TATA 依赖性和 TATA 非依赖性早期启动子活动。  |  Kogan, PH., et al. 1995. J Virol. 69: 1452-61. PMID: 7853477
  7. 宿主转录因子与 CACGTG 和 GATA 元件结合可激活杆状病毒 gp64 早期启动子。  |  Kogan, PH. and Blissard, GW. 1994. J Virol. 68: 813-22. PMID: 8289385
  8. 杆状病毒 gp64 包膜融合蛋白基因的后期启动子选择。  |  Garrity, DB., et al. 1997. Virology. 231: 167-81. PMID: 9168879
  9. 杆状病毒 gp64 基因的表达:小分子的负调控。  |  Chang, MJ. and Blissard, GW. 1997. J Virol. 71: 7448-60. PMID: 9311824

订购信息

产品名称产品编号规格价格数量收藏夹

Baculovirus gp64 抗体 (AcV1)

sc-65498
200 µg/ml
$316.00

Baculovirus gp64 (AcV1): m-IgG Fc BP-HRP 套装

sc-528643
200 µg Ab; 10 µg BP
$354.00

Baculovirus gp64 (AcV1): m-IgGκ BP-HRP 套装

sc-521076
200 µg Ab, 40 µg BP
$354.00

Baculovirus gp64 抗体 (AcV1) AC

sc-65498 AC
500 µg/ml, 25% agarose
$416.00

Baculovirus gp64 抗体 (AcV1) HRP

sc-65498 HRP
200 µg/ml
$316.00

Baculovirus gp64 抗体 (AcV1) FITC

sc-65498 FITC
200 µg/ml
$330.00

Baculovirus gp64 抗体 (AcV1) PE

sc-65498 PE
200 µg/ml
$343.00

Baculovirus gp64 抗体 (AcV1) Alexa Fluor® 488

sc-65498 AF488
200 µg/ml
$357.00

Baculovirus gp64 抗体 (AcV1) Alexa Fluor® 546

sc-65498 AF546
200 µg/ml
$357.00

Baculovirus gp64 抗体 (AcV1) Alexa Fluor® 594

sc-65498 AF594
200 µg/ml
$357.00

Baculovirus gp64 抗体 (AcV1) Alexa Fluor® 647

sc-65498 AF647
200 µg/ml
$357.00

Baculovirus gp64 抗体 (AcV1) Alexa Fluor® 680

sc-65498 AF680
200 µg/ml
$357.00

Baculovirus gp64 抗体 (AcV1) Alexa Fluor® 790

sc-65498 AF790
200 µg/ml
$357.00

We are interested in potentially using this antibody AcV1 sc-65498 and AcV5 sc-65499 as capture and detection antibodies in a GP64 quantitation ELISA. Do you have any resources that might support this application?

Asked by: Ben L
Thank you for your question. For assistance please contact Technical Service. Please call 800-457-3801 or email scbt@scbt.com.
Answered by: BlakeJ
Date published: 2025-07-17

We are interested in using AcV1 sc-65498 AcV5 sc-65499 as capture and detection antibodies in a GP64 quantitation ELISA. Do you have any resources that would support this application?

Asked by: Ben L
Thank you for your question. For assistance please contact Technical Service. Please call 800-457-3801 or email scbt@scbt.com.
Answered by: BlakeJ
Date published: 2025-07-17

Suggest Isotype catalog # to use in Western blot

Asked by: Lakshmi
Thank you for your question. https://www.scbt.com/p/normal-mouse-igg2a
Answered by: BlakeJ
Date published: 2025-04-04

Can I use Baculovirus gp64 Antibody (AcV1) for double staining if my other primary antibody was raised in mice?

Asked by: Trav11
Yes, you can use the Baculovirus gp64 Antibody (AcV1): sc-65498 for double staining with another mouse primary antibody. In this case, we just recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, the Baculovirus gp64 Antibody (AcV1): sc-65498 is available conjugated to HRP (sc-65498 HRP), phycoerythrin (sc-65498 PE), fluoroscein (sc-65498 FITC), Alexa Fluor® 488 (sc-65498 AF488) or Alexa Fluor® 647 (sc-65498 AF647).
Answered by: Tech Service 8
Date published: 2016-12-16

What secondary reagent should I use for detection of Baculovirus gp64 Antibody (AcV1) via Western blot?

Asked by: Trav11
We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, such as m-IgGκ BP-HRP: sc-516102, for Western blot. A complete list of all available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 8
Date published: 2016-12-16

Do you have one lot of this product? How often do you change lot numbers, if not?  Thanks in advance.

Asked by: Erin Shepherd
Thank you for your inquiry. Please contact our Technical Service department for availability and inventory information, by phone at 800-457-3801 or by e-mail at scbt@scbt.com
Answered by: Technical Service
Date published: 2016-11-01
  • y_2025, m_8, d_28, h_5CST
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Rated 4 out of 5 by from Only detects non-denatured GP64This Western blot will not detect denatured (in SDS + beta-mercaptoethanol) GP64. See publication: "Mapping the Conformational Epitope of a Neutralizing Antibody (AcV1) Directed Against the AcMNPV GP64 Protein" by Jian Zhou and Gary Blissard 2013.
Date published: 2023-01-19
Rated 5 out of 5 by from A must-have for flow cytometry.A great choice for Baculovirus detection. Recommend for flow cytometry and has 9 reviews to support it.
Date published: 2016-12-23
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Baculovirus gp64 抗体 (AcV1) is rated 4.7 out of 5 by 3.
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