Date published: 2025-9-8
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APG5/ATG5 Antibody (C-1) - Western Blotting - Image 352054
ATG5 抗体 (C-1): sc-133158. PANC-1 (A), NIH/3T3 (B), CCRF-CEM (C), HT-1080 (D) 和 U-87 MG (E) 全细胞裂解液中 ATG5-Atg12 结合物表达的 Western 印迹分析.
ATG5 抗体 (C-1): sc-133158. PANC-1 (A), NIH/3T3 (B), CCRF-CEM (C), HT-1080 (D) 和 U-87 MG (E) 全细胞裂解液中 ATG5-Atg12 结合物表达的 Western 印迹分析.

APG5/ATG5 抗体 (C-1): sc-133158

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寫評論提問

说明书
  • APG5/ATG5 抗体 C-1 是小鼠单克隆 IgG2a κ，APG5/ATG5抗体, 在103篇文献中引用，规格为200 µg/ml
  • 免疫human物种的代表全长ATG5的氨基酸1-275
  • APG5/ATG5 抗体 (C-1) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 ATG5
  • 抗APG5/ATG5抗体（C-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取APG5/ATG5 (C-1): sc-133158的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 APG5/ATG5 抗体 (C-1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与APG5/ATG5 抗体 (C-1) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 APG 抗体 包括 ATG7

ATG5抗体（C-1）是一种IgG2a κ小鼠单克隆ATG5抗体（也称为自噬相关5抗体，或APG5抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的ATG5蛋白。ATG5抗体（C-1）既可以是非偶联的抗ATG5抗体形式，也可以是多种偶联形式的抗ATG5抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。在酵母中，自噬是营养饥饿和细胞分化过程中生存所必需的过程。自噬过程的特点是非选择性地将细胞质蛋白降解为称为自噬体的膜结构，它依赖于几种蛋白质，包括自噬蛋白ATG5（APG5）和APG7。酵母Apg7和人类同源物APG7与酿酒酵母中的泛素激活酶E1具有相似性，同样负责酶促激活自噬偶联系统。Apg5和人类同源物ATG5（也称为APG5、凋亡特异性蛋白或APS）作为自噬蛋白Atg12的底物发挥作用。这些蛋白质通过共价键结合在一起，形成Atg12/ATG5偶联物，这是自噬进展所必需的。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

APG5/ATG5 抗体 (C-1) 参考文献:

  1. 参与酵母自噬的基因 APG5 的结构和功能分析。  |  Kametaka, S., et al. 1996. Gene. 178: 139-43. PMID: 8921905
  2. 向酵母液泡的非经典蛋白质分拣  |  Klionsky, DJ. 1998. J Biol Chem. 273: 10807-10. PMID: 9556549
  3. 人类凋亡特异性蛋白与酵母中参与自噬的基因 APG5 的产物之间的同源性。  |  Hammond, EM., et al. 1998. FEBS Lett. 425: 391-5. PMID: 9563500
  4. 自噬所必需的蛋白质连接系统  |  Mizushima, N., et al. 1998. Nature. 395: 395-8. PMID: 9759731
  5. 人类新的蛋白质连接系统。酵母 Apg12p 连接系统的对应物对自噬至关重要。  |  Mizushima, N., et al. 1998. J Biol Chem. 273: 33889-92. PMID: 9852036

订购信息

产品名称产品编号规格价格数量收藏夹

APG5/ATG5 抗体 (C-1)

sc-133158
200 µg/ml
$316.00

APG5/ATG5 (C-1): m-IgGκ BP-HRP 套装

sc-521255
200 µg Ab, 40 µg BP
$354.00

APG5/ATG5 (C-1): m-IgG2a BP-HRP 套装

sc-546093
200 µg Ab; 10 µg BP
$354.00

APG5/ATG5 抗体 (C-1) AC

sc-133158 AC
500 µg/ml, 25% agarose
$416.00

APG5/ATG5 抗体 (C-1) HRP

sc-133158 HRP
200 µg/ml
$316.00

APG5/ATG5 抗体 (C-1) FITC

sc-133158 FITC
200 µg/ml
$330.00

APG5/ATG5 抗体 (C-1) PE

sc-133158 PE
200 µg/ml
$343.00

APG5/ATG5 抗体 (C-1) Alexa Fluor® 488

sc-133158 AF488
200 µg/ml
$357.00

APG5/ATG5 抗体 (C-1) Alexa Fluor® 546

sc-133158 AF546
200 µg/ml
$357.00

APG5/ATG5 抗体 (C-1) Alexa Fluor® 594

sc-133158 AF594
200 µg/ml
$357.00

APG5/ATG5 抗体 (C-1) Alexa Fluor® 647

sc-133158 AF647
200 µg/ml
$357.00

APG5/ATG5 抗体 (C-1) Alexa Fluor® 680

sc-133158 AF680
200 µg/ml
$357.00

APG5/ATG5 抗体 (C-1) Alexa Fluor® 790

sc-133158 AF790
200 µg/ml
$357.00

Hi I want to know that this antibody can detect the ATG5-ATG12 complexor not? I am highly thankfull for your kind reply.

Asked by: Priyanka Mishra
Thank you for your question. Based on the observed molecular weight of ~ 55 kDa in the cell lines tested by Western blot with this antibody, and the reported observed molecular weight of ~ 55 kDa for the ATG5-ATG12 complex reported in the literature, (e.g., PMID: 19783656), we believe this antibody can detect the ATG5-ATG12 complex.
Answered by: Technical Support
Date published: 2019-03-19

您好，这个是自噬相关基因ATG5吗？研究大鼠心肌 能够完成WB 石蜡切片的免疫组化DAB和荧光吗 是否也能完成免疫共沉淀

Asked by: 悦悦悦悦
感谢您的提问。请联系我们的技术支持部门。亚洲技术支持电话(+86) 021-60936350、邮箱asia@scbio.cn、或选择网页上Live Chat中的“中文支持”。 Thank you for your inquiry. Please contact our Technical Service Department. Our Asian Technical Service team is available at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Technical Support 8
Date published: 2018-12-21

What secondary is best to use with APG5 (C-1): sc-133158 monoclonal antibody?

Asked by: jenniferc
Thank you for your question. While most anti-mouse IgG secondary antibodies are appropriate for use with APG5 (C-1): sc-133158, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 15
Date published: 2017-01-11
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Rated 4 out of 5 by from 还可以 比较稳定条带还可以 特异性条带较少，basically good can be used in MDA-MB-231 cell line, don't incubate too long
Date published: 2022-06-30
Rated 5 out of 5 by from Good for WB and ICCThis antibody works well. I tried with 1:2000 for ICC, and 1:4000 for WB. Both are great.
Date published: 2022-06-28
Rated 4 out of 5 by from Buon anticorpoUsed 1: 200 it worked very well, I did not find any aspecific bands and the result was very clean (using 20 ug of protein)
Date published: 2021-01-26
Rated 3 out of 5 by from Varias bandas detectadas.Aparecen varias bandas. Unas en peso molecular. Muestras de células estrelladas.
Date published: 2020-05-09
Rated 4 out of 5 by from good antibodyone clear clean band at 55 with western blot analysis
Date published: 2020-02-06
Rated 4 out of 5 by from Antibody works wellThis antibody works well. I had to use a lower dilution than with some other antibodies that I have purchased from Santa Cruz. I had to use a 1:250 dilution.
Date published: 2019-05-07
Rated 4 out of 5 by from works wellWe tried with 1:1000 in mouse fibroblasts. It works well. Weak but clear bands.
Date published: 2018-10-10
Rated 4 out of 5 by from Good for WBGreat for WB, very clean bands. Highly recommend this antibody.
Date published: 2017-12-30
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APG5/ATG5 抗体 (C-1) is rated 4.6 out of 5 by 35.
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