Date published: 2025-9-7
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ASC/TMS1/PYCARD Antibody (F-9) - Western Blotting - Image 134810
ASC 抗体 (F-9): sc-271054. SK-MEL-28 (A) 和 K-562 (B) 全细胞裂解液中 ASC 表达的 Western 印迹分析.
ASC 抗体 (F-9): sc-271054. SK-MEL-28 (A) 和 K-562 (B) 全细胞裂解液中 ASC 表达的 Western 印迹分析.

ASC/TMS1/PYCARD 抗体 (F-9): sc-271054

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说明书
  • ASC/TMS1/PYCARD抗体(F-9)是小鼠单克隆IgG1 κ, 在115篇文献中引用，规格为200 µg/ml
  • 针对氨基酸1-120的抗原，在ASC的N-terminus处映射，human来源
  • 抗-ASC/TMS1/PYCARD 抗体 (F-9) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 ASC
  • 抗ASC/TMS1/PYCARD抗体（F-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取ASC/TMS1/PYCARD (F-9): sc-271054的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 ASC/TMS1/PYCARD 抗体 (F-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与ASC/TMS1/PYCARD 抗体 (F-9) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 ASC 抗体包括 ASC2

ASC 抗体 (F-9) 是一种小鼠单克隆 IgG1 kappa 轻链抗体，其靶标是含 CARD（ASC）的凋亡相关斑点样蛋白，也称为 TMS1 或 PYCARD。抗 ASC 抗体（F-9）是针对人 ASC 的 N 端 1-120 氨基酸制备的，能有效地检测小鼠、大鼠和人体内的 ASC 蛋白，检测方法包括西部印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附试验（ELISA）。ASC 是含 CARD 的适配蛋白家族的重要成员，通过介导 Apaf-1 等适配蛋白与 CASP9 等促天冬酶之间的相互作用，在凋亡信号传导中发挥着关键作用。从结构上看，ASC 的 N 端包括一个PYD 样结构域（Pyrin-like domain），C 端包括一个 87 位氨基酸的 CARD 样结构域（Caspase recruitment domain），这两个结构域对其凋亡调控功能至关重要。诱导细胞凋亡时，ASC 会从弥散的细胞质分布转变为形成点状或球状的核周聚集体，这一过程可通过荧光显微镜观察到。这种聚集在细胞对凋亡刺激（包括维甲酸和各种抗肿瘤药物）的反应中非常重要。此外，在多种白血病和黑色素瘤细胞系中也观察到了 ASC 的表达，这表明它有可能成为癌症研究中的一种生物标记物。ASC (F-9) 抗体有非共轭型和多种共轭型，包括辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 变体，是研究人员研究细胞凋亡机制和相关细胞通路的多功能基本工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ASC/TMS1/PYCARD 抗体 (F-9) 参考文献:

  1. ASC 是一种新型 22 kDa 蛋白，在人类早幼粒细胞白血病 HL-60 细胞凋亡过程中聚集。  |  Masumoto, J., et al. 1999. J Biol Chem. 274: 33835-8. PMID: 10567338
  2. TMS1是一种新型促凋亡Caspase招募结构域蛋白，是人类乳腺癌中甲基化诱导基因沉默的靶标。  |  Conway, KE., et al. 2000. Cancer Res. 60: 6236-42. PMID: 11103776
  3. 新型 Caspase 招募结构域蛋白 TMS1 的亚细胞再分布激活了 Caspase-9 介导的细胞凋亡途径。  |  McConnell, BB. and Vertino, PM. 2000. Cancer Res. 60: 6243-7. PMID: 11103777
  4. pyrin 结构域：与细胞凋亡和炎症有关的死亡结构域-折叠家族的可能成员。  |  Martinon, F., et al. 2001. Curr Biol. 11: R118-20. PMID: 11250163
  5. ASC斑形成是炎症小体活化的读数。  |  Stutz, A., et al. 2013. Methods Mol Biol. 1040: 91-101. PMID: 23852599
  6. ASC斑的组装和调节。  |  Hoss, F., et al. 2017. Cell Mol Life Sci. 74: 1211-1229. PMID: 27761594
  7. 全面回顾免疫稳态和疾病中ASC的结构和功能。  |  Agrawal, I. and Jha, S. 2020. Mol Biol Rep. 47: 3077-3096. PMID: 32124174
  8. NLRP3炎症小体/ASC斑点和淀粉样蛋白聚集之间的相互作用会加速阿尔茨海默氏症和帕金森氏症的病情发展。  |  Hulse, J. and Bhaskar, K. 2022. Front Mol Neurosci. 15: 805169. PMID: 35185469
  9. 灰色区域鳞状病变：Asc-US/Asc-H。  |  Kattoor, J. and Kamal, MM. 2022. Cytojournal. 19: 30. PMID: 35673698
  10. ASC 通过 p53/AMPKα 轴调节皮下脂肪组织的脂肪生成和脂肪分解  |  Chen, H., et al. 2022. Int J Mol Sci. 23: PMID: 36077447
  11. ASC斑点通过扩大NLRP3炎症小体的活性而加剧α-突触核蛋白的病理变化。  |  Zheng, R., et al. 2023. J Neuroinflammation. 20: 26. PMID: 36740674
  12. 内源性ASC斑的纳米级组织。  |  Glück, IM., et al. 2023. iScience. 26: 108382. PMID: 38047065

订购信息

产品名称产品编号规格价格数量收藏夹

ASC/TMS1/PYCARD 抗体 (F-9)

sc-271054
200 µg/ml
$316.00

ASC/TMS1/PYCARD (F-9): m-IgG Fc BP-HRP 套装

sc-529106
200 µg Ab; 10 µg BP
$354.00

ASC/TMS1/PYCARD (F-9): m-IgGκ BP-HRP 套装

sc-521830
200 µg Ab, 40 µg BP
$354.00

ASC/TMS1/PYCARD (F-9): m-IgG1 BP-HRP 套装

sc-543322
200 µg Ab; 20 µg BP
$354.00

ASC/TMS1/PYCARD 抗体 (F-9) AC

sc-271054 AC
500 µg/ml, 25% agarose
$416.00

ASC/TMS1/PYCARD 抗体 (F-9) HRP

sc-271054 HRP
200 µg/ml
$316.00

ASC/TMS1/PYCARD 抗体 (F-9) FITC

sc-271054 FITC
200 µg/ml
$330.00

ASC/TMS1/PYCARD 抗体 (F-9) PE

sc-271054 PE
200 µg/ml
$343.00

ASC/TMS1/PYCARD 抗体 (F-9) Alexa Fluor® 488

sc-271054 AF488
200 µg/ml
$357.00

ASC/TMS1/PYCARD 抗体 (F-9) Alexa Fluor® 546

sc-271054 AF546
200 µg/ml
$357.00

ASC/TMS1/PYCARD 抗体 (F-9) Alexa Fluor® 594

sc-271054 AF594
200 µg/ml
$357.00

ASC/TMS1/PYCARD 抗体 (F-9) Alexa Fluor® 647

sc-271054 AF647
200 µg/ml
$357.00

ASC/TMS1/PYCARD 抗体 (F-9) Alexa Fluor® 680

sc-271054 AF680
200 µg/ml
$357.00

ASC/TMS1/PYCARD 抗体 (F-9) Alexa Fluor® 790

sc-271054 AF790
200 µg/ml
$357.00

I'd like to know if this product is recombinant Ab or purified from culture supernatant of hybridoma

Asked by: bob27
Thank you for your question. ASC/TMS1/PYCARD Antibody (F-9): sc-271054 is supplied as 200 µg purified antibody from cell culture in 1 ml PBS buffer.
Answered by: Tech Support Europe
Date published: 2025-05-26

Can you please direct me to the paper or in-house data for the use of this antibody in ELISAs?

Asked by: JC01
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Answered by: Dallas Tech Service
Date published: 2022-02-22

Is the antibody available in INDIA.

Asked by: Uday
Thank you for your question. Please contact one of our authorized distributors in India to request a quote for this antibody. A link to search for their contact information on our website is provided below. https://www.scbt.com/scbt/resources/distributors
Answered by: Technical Support
Date published: 2017-10-05

Is ASC (F-9): sc-271054 monoclonal antibody available directly conjugated to HRP or fluorescent dyes?

Asked by: jenni
Thank you for your question. All requests for directly conjugated versions of this antibody should be directed to our Special Orders team at specialorders@scbt.com Alternatively, consider using ASC (B-3): sc-514414, which is available conjugated to agarose, HRP, FITC, PE, and more. (https://www.scbt.com/scbt/product/asc-antibody-b-3)
Answered by: Technical Support 9
Date published: 2017-01-10
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Rated 4 out of 5 by from ASCASC antibody used for western blot analysis of mouse and human lens/Lens epithelial cells protein
Date published: 2024-07-05
Rated 5 out of 5 by from Good antibodyWe purchased this antibody that conjugated with Alexa Fluor 546 for the detection of ASC speck. We can see a number of ASC specks in human macrophages treated with ATP control, but not untreated cells when cells were stained with this antibody after treatment. I recommend it!
Date published: 2021-06-22
Rated 5 out of 5 by from GOO result in Western blotWe used this product in WB, with good result. The band was clearly developed.
Date published: 2020-09-01
Rated 5 out of 5 by from ASC Antibody (F-9) wAorked for inflammasome specksASC Antibody (F-9) worked for inflammasome specks detection
Date published: 2019-12-04
Rated 4 out of 5 by from it is okI receive this about half year ago, with another product usp4. Beyong quetion, we had some positive result，it is ok. And honestly，I do not how to evaluate it is quality. That is all.
Date published: 2018-04-04
Rated 3 out of 5 by from buen resultadoprobamos el anticuerpo en tejido de conejo y se reconoció una banda a alto peso molecular que podría corresponder con el acoplamiento de ASC a otra proteína del complejo que forma al activarse
Date published: 2017-12-12
Rated 5 out of 5 by from Strong western and IHCGreat product for IHC staining; also strong WB data
Date published: 2017-01-14
Rated 5 out of 5 by from Great, versatile antibodyThe ASC Antibody (F-9): sc-271054 works well for a variety of applications including WB, IF, IHC(P), and more. It has been cited multiple times for further verification.
Date published: 2017-01-10
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ASC/TMS1/PYCARD 抗体 (F-9) is rated 4.5 out of 5 by 13.
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