ARK-1 Antibody (C-1): sc-398814

ARK-1 Antibody (C-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects ARK-1 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). ARK-1 (C-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Aurora related kinase-1 (ARK-1), also known as STK15, Aurora2, and Aik1, is a centrosome-associated serine/threonine kinase that plays a crucial role in regulating centrosome separation, bipolar spindle assembly, and chromosome segregation during mitosis. Proper functioning of ARK-1 is vital for accurate cell division, as dysregulation can lead to aneuploidy and cancer. ARK-1 is predominantly localized in the nucleus and associates with distinct components of the mitotic machinery, particularly at the spindle poles during mitosis. ARK-1 protein levels are elevated in various colon carcinoma cell lines, such as HT-29 and SW480, and accumulates during metaphase in HeLa cells, highlighting potential as a biomarker for tumor progression. ARK-1 undergoes degradation after the G2/M phase via the ubiquitin-proteasome pathway, which is essential for maintaining proper cell cycle regulation. Expression of ARK-1 is particularly high in the human thymus and fetal liver, indicating importance in developmental processes.

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ARK-1 Antibody (C-1) References:

1. Identification of Novel Aurora Kinase A (AURKA) Inhibitors via Hierarchical Ligand-Based Virtual Screening.  |  Kong, Y., et al. 2018. J Chem Inf Model. 58: 36-47. PMID: 29202231
2. Aurora kinase A (AURKA) promotes the progression and imatinib resistance of advanced gastrointestinal stromal tumors.  |  Cheng, X., et al. 2021. Cancer Cell Int. 21: 407. PMID: 34332577
3. Aurora kinase A regulates liver regeneration through macrophages polarization and Wnt/β-catenin signalling.  |  Yin, Y., et al. 2022. Liver Int. 42: 468-478. PMID: 34719108
4. Aurora Kinase A as a Diagnostic and Prognostic Marker of Malignant Mesothelioma.  |  Guo, Z., et al. 2021. Front Oncol. 11: 789244. PMID: 34956905
5. Targeting aurora kinase a (AURKA) in cancer: molecular docking and dynamic simulations of potential AURKA inhibitors.  |  Almilaibary, A. 2022. Med Oncol. 39: 246. PMID: 36180808
6. The feedback loop of AURKA/DDX5/TMEM147-AS1/let-7 drives lipophagy to induce cisplatin resistance in epithelial ovarian cancer.  |  Shao, Y., et al. 2023. Cancer Lett. 565: 216241. PMID: 37217070
7. Aurora kinase A regulates cancer-associated RNA aberrant splicing in breast cancer.  |  Li, S., et al. 2023. Heliyon. 9: e17386. PMID: 37415951
8. eEF1A2 promotes PTEN-GSK3β-SCF complex-dependent degradation of Aurora kinase A and is inactivated in breast cancer.  |  Treekitkarnmongkol, W., et al. 2024. Sci Signal. 17: eadh4475. PMID: 38442201
9. TonEBP inhibits ciliogenesis by controlling aurora kinase A and regulating centriolar satellite integrity.  |  Chinbold, B., et al. 2024. Cell Commun Signal. 22: 348. PMID: 38961488
10. Caspase-mediated AURKA cleavage at Asp132 is essential for paclitaxel to elicit cell apoptosis.  |  Chen, X., et al. 2024. Theranostics. 14: 3909-3926. PMID: 38994036

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ARK-1 Antibody (C-1) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-ARK-1 Antibody (C-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ARK-1 Antibody (C-1) Specifications

Alternate Product Name: ARK-1

Product Clone Name: C-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-130

Epitope Target: ARK-1

Epitope Species: human

Product Reactivity/Detection: ARK-1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for ARK-1 Antibody (C-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred ARK-1 antibody is ARK-1 Antibody (35C1): sc-56881.