ARID3A Double Nickase Plasmid (h): sc-404552-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ARID3A Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ARID3A Double Nickase Plasmid (h) and ARID3A Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ARID3A. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ARID3A Antibody (A-4): sc-398367
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ARID3A Double Nickase Plasmid (h) | sc-404552-NIC | 20 µg | $410.00 | |||
ARID3A Double Nickase Plasmid (h2) | sc-404552-NIC-2 | 20 µg | $410.00 |
ARID3A (AT-rich interaction domain 3A) encodes a DNA-binding transcription factor of the ARID family that recognizes AT-rich regulatory elements and helps shape chromatin-dependent control of gene expression. It participates in lineage-associated transcriptional programs, with prominent roles in hematopoietic and B-cell differentiation, immunoglobulin gene regulation, and modulation of cell-cycle and apoptotic pathways. Through interactions with cofactors and chromatin remodeling machinery, ARID3A influences transcriptional networks linked to proliferation, immune signaling, and cellular identity. Dysregulated ARID3A expression or function has been associated with altered immune cell development and oncogenic transcriptional states in hematologic and solid tumor contexts, making it a useful node for mechanistic studies of transcriptional control.
ARID3A Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ARID3A locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ARID3A. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ARID3A function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ARID3A-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.