Date published: 2025-9-7
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Arc Antibody (C-7) - Western Blotting - Image 4532
Arc 抗体 (C-7): sc-17839. U-87 MG (A) 和 SH-SY5Y (B) 全细胞裂解液及小鼠脑组织提取物 (C) 中 Arc 表达的 Western 印迹分析.
Arc 抗体 (C-7): sc-17839. U-87 MG (A) 和 SH-SY5Y (B) 全细胞裂解液及小鼠脑组织提取物 (C) 中 Arc 表达的 Western 印迹分析.

Arc 抗体 (C-7): sc-17839

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说明书
  • Arc 抗体 C-7 是小鼠单克隆 IgG2a κ，Arc抗体, 在165篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Arc (activity-regulated cytoskeleton-associated protein)的氨基酸1-300
  • Arc 抗体 (C-7) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 ARC
  • 抗Arc抗体（C-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Arc (C-7): sc-17839的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 2a BP-HRP">m-IgG2a BP-HRP是Arc Antibody (C-7) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Arc Antibody (C-7) 打包提供（请参阅下面的订购信息）。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

Arc抗体（C-7）是一种小鼠单克隆IgG2a kappa轻链抗体，通过识别人类Arc蛋白的氨基酸1-300来靶向Arc蛋白，该蛋白也被称为活性调节细胞骨架相关蛋白或Arg3.1。抗Arc抗体（C-7）可检测多种物种（包括小鼠、大鼠和人类）的Arc，适用于多种应用，如蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）。Arc蛋白是一种重要的早期基因，参与突触可塑性、学习和记忆，在调节细胞骨架和响应神经元活动调节突触强度方面发挥着关键作用。生长因子和神经递质可快速诱导Arc表达，从而促进认知功能所需的长期细胞反应和神经可塑性变化。Arc主要定位于海马、杏仁核、下丘脑、纹状体和大脑皮层等关键大脑区域的神经元细胞体和树突中，与F-肌动蛋白和其他肌动蛋白相关蛋白相互作用，影响树突棘形态和突触效能。Arc (C-7) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物，为各种实验设置提供了多种工具。此外，Arc表达与可卡因等刺激的反应有关，这强调了Arc在活动依赖性基因调控中的作用及其在神经系统疾病中的潜在意义。Arc单克隆抗体（C-7）使研究人员能够深入研究Arc蛋白在各种生物学环境中的动态调控，从而加深我们对神经可塑性和相关病理的认识。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Arc 抗体 (C-7) 参考文献:

  1. 刺激神经元乙酰胆碱受体可诱导基因快速转录。  |  Greenberg, ME., et al. 1986. Science. 234: 80-3. PMID: 3749894
  2. 在plysia的长期异突触促进过程中，大分子合成是一个关键时期。  |  Montarolo, PG., et al. 1986. Science. 234: 1249-54. PMID: 3775383
  3. 大鼠大脑中可卡因对弧的激活，弧是一种推定的 '效应 '即刻早期基因。  |  Fosnaugh, JS., et al. 1995. J Neurochem. 64: 2377-80. PMID: 7722525
  4. Arc是一种生长因子和活性调节基因，它编码一种新的细胞骨架缔合作用蛋白，这种蛋白富集在神经元树突中。  |  Lyford, GL., et al. 1995. Neuron. 14: 433-45. PMID: 7857651

订购信息

产品名称产品编号规格价格数量收藏夹

Arc 抗体 (C-7)

sc-17839
200 µg/ml
$316.00

Arc (C-7): m-IgG Fc BP-HRP 套装

sc-525495
200 µg Ab; 10 µg BP
$354.00

Arc (C-7): m-IgG2a BP-HRP 套装

sc-546288
200 µg Ab; 10 µg BP
$354.00

Arc 抗体 (C-7) AC

sc-17839 AC
500 µg/ml, 25% agarose
$416.00

Arc 抗体 (C-7) HRP

sc-17839 HRP
200 µg/ml
$316.00

Arc 抗体 (C-7) FITC

sc-17839 FITC
200 µg/ml
$330.00

Arc 抗体 (C-7) PE

sc-17839 PE
200 µg/ml
$343.00

Arc 抗体 (C-7) Alexa Fluor® 488

sc-17839 AF488
200 µg/ml
$357.00

Arc 抗体 (C-7) Alexa Fluor® 546

sc-17839 AF546
200 µg/ml
$357.00

Arc 抗体 (C-7) Alexa Fluor® 594

sc-17839 AF594
200 µg/ml
$357.00

Arc 抗体 (C-7) Alexa Fluor® 647

sc-17839 AF647
200 µg/ml
$357.00

Arc 抗体 (C-7) Alexa Fluor® 680

sc-17839 AF680
200 µg/ml
$357.00

Arc 抗体 (C-7) Alexa Fluor® 790

sc-17839 AF790
200 µg/ml
$357.00

I have tested this antibody in WB and IF. Works very well in both at 1:500. However, we are detecting two strong bands in WB around 55kDa. The second band is heavier and less abundant than the lighter band. What could this be? its too light to be dimer 

Asked by: BenitoNeuro
Thank you for your question. For a discussion please contact technical service by emailing scbt@scbt.com or call 800-457-3801.
Answered by: BlakeJ
Date published: 2024-01-24

Hi, Can I use Arc Antibody (C-7) HRP for ELISA to detect low abundant of Arc in mouse tissue? Do you have a protocol for this? Thank you.

Asked by: Jackie2021
Thank you for your question. We have not yet tested this Arc Antibody (C-7): sc-17839 for ELISA in cells or tissues. We do recommend and warranty the antibody for ELISA based on reactivity of the antibody in other applications, as well as for indirect ELISA against the antibody’s antigen. Unfortunately, we do not have a protocol for using the HRP conjugated Arc antibody in ELISA. We recommend searching online for ELISA protocols using HRP conjugated antibodies for detection in tissues.
Answered by: Technical Support
Date published: 2020-03-30

Hi, I was wondering if this antibody has been tested for IHC in ferret brain. Thank you!

Asked by: Silei
Thank you for your question. This antibody has not yet been tested in ferret and so that is not a recommended species at this time. It may work, but we can not warranty it for that use. We are still gathering data to show on our website. Check back for updates!
Answered by: Tech Service
Date published: 2019-12-13

Can I use this antibody for immunostaining in HEK29 cells and would it be necessary to perform antigen retrieval?

Asked by: Donya
Thank you for your question. This antibody has not yet been tested for IHC on paraffin tissue sections, so we can not recommend it for that use. It is recommended for IF and IHC on fresh frozen tissue samples. If you do want to try it on paraffin embedded tissues, i would highly recommend performing an antigen retrieval step.
Answered by: Tech Service
Date published: 2019-09-13

Arc Antibody (C-7) AC - What does AC refer to?

Asked by: dabrus
Thank you for your question. The AC stands for "agarose conjugate" for immunoprecipitation. This mouse monoclonal, Arc (C-7): sc-17839 is available conjugated to agarose (sc-17839 AC), 500 µg/0.25 ml agarose in 1 ml.
Answered by: Technical Support
Date published: 2020-02-26

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: DefinitelyNotMatt
Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is currently available conjugated to HRP (sc-17839 HRP), phycoerythrin (sc-17839 PE), fluorescein (sc-17839 FITC), Alexa Fluor® 488 (sc-17839 AF488) or Alexa Fluor® 647 (sc-17839 AF647).
Answered by: Tech Service 9
Date published: 2016-12-15

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: DefinitelyNotMatt
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 9
Date published: 2016-12-14

Please send a quote for Arc (C-7): sc-17839 in AUD with an approximate shipping date

Asked by: Jacque
Thank you for your question. Please contact our Customer Care department, or one of our authorized distributors in Australia for a complete quote including shipping information. Links are provided below for our contact information. https://www.scbt.com/scbt/customer-care/contact-us https://www.scbt.com/scbt/resources/distributors
Answered by: Technical Support
Date published: 2016-12-14
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Rated 3 out of 5 by from 使用protein A or G as Co-IP beads使用protein A or G as Co-IP beads, there is no recommondation about the type of beads for Coip
Date published: 2022-08-20
Rated 1 out of 5 by from diffused bands are obtainedNewly purchased antibody, still not giving specific bands during immunoblotting.
Date published: 2020-11-05
Rated 1 out of 5 by from Has deteriorated and does not work wellThis anttibody worked beautifully for us in the past but recent batches have many non-specific and even with strogn stimulations is impossible to dectect the signal.
Date published: 2020-10-20
Rated 4 out of 5 by from Western blot- brain tissueWorked well for Western blots of brain tissue and neuronal cell cultures. Did not work for IF in neuronal cultures or tissue.
Date published: 2020-01-07
Rated 1 out of 5 by from It did not work well in IF我在使用这个抗体进行免疫组化时候，尽管有些细胞被轻微标记出来，但是背景很深，与同时用的别家抗体形成巨大反差，别家抗体很清晰，背景跟细胞分辨很清楚，这个抗体几乎分辨不出来目的细胞与背景，请问是什么原因，另外下面采用这个抗体的论文，你们写着有一篇使用的这个抗体，示意图也很好，但是我仔细看了那篇文章发现用的不是这个抗体，我是因为你们的例子这个才买的你们的抗体，
Date published: 2018-09-15
Rated 4 out of 5 by from IF in hippocampal neuronsit has been tested on dissociated hippocampal neurons in a IF assay. The staining seem to be specific and the signal was well detectable.
Date published: 2018-02-23
Rated 5 out of 5 by from Good antibody for WBWorked very well in WB at 1:500 dilution with mouse brain tissue lysate.
Date published: 2017-07-25
Rated 4 out of 5 by from Works for immunofluorescenceThe antibody works for immunofluorescence of fixed-frozen floating sections (brain). Blocking with Mouse on Mouse kit reduces signal:noise.
Date published: 2017-05-27
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Arc 抗体 (C-7) is rated 4.1 out of 5 by 22.
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