Date published: 2025-9-6
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AP-2α Antibody (3B5) - Western Blotting - Image 354842
AP-2α (3B5): sc-12726. ZR-75-1 (A), JAR (B) 和 RPE-J (C) 全细胞裂解液中 AP-2α 表达的 Western 印迹分析.
AP-2α (3B5): sc-12726. ZR-75-1 (A), JAR (B) 和 RPE-J (C) 全细胞裂解液中 AP-2α 表达的 Western 印迹分析.

AP-2α 抗体 (3B5): sc-12726

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说明书
  • AP-2 alpha 抗体 3B5 是小鼠单克隆 IgG2b κ，AP-2α抗体, 在100篇文献中引用，规格为200 µg/ml
  • 抗-AP-2 alpha 抗体 (3B5) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 AP-2α
  • AP-2 alpha抗体（3B5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-12726 X, 200 µg/0.1 ml)
  • 关于如何获取AP-2α (3B5): sc-12726的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgGκ BP-HRP 是 AP-2α 抗体 (3B5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与AP-2α 抗体 (3B5) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Activating Enhancer-Binding Protein 抗体 包括 AP-2alpha, AP-2β, AP-2 γ和AP-4

AP-2α抗体（3B5）是一种小鼠单克隆IgG2b kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学（IHC）检测小鼠、大鼠和人类来源的AP-2α蛋白。AP-2α单克隆抗体（3B5）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种Alexa Fluor®结合型。AP-2α蛋白是一种关键的转录因子，它与DNA的CCCAGGC序列结合，启动特定基因的转录。这一功能对于调节与细胞生长和分化相关的基因表达至关重要，特别是在外胚层衍生组织中。AP-2α (3B5) 抗体参与调节雌激素受体的表达，并在各种组织的发展中发挥重要作用，AP-2α基因的突变与严重发育障碍有关，如小眼球和角膜混浊。AP-2α与AP-2转录因子家族的其他成员（包括AP-2β和AP-2γ）相互作用，共同形成OB2-1转录因子复合物，上调原癌基因c-ErbB-2的表达，而该基因在乳腺癌中通常过度表达。AP-2α能够调节基因表达，这凸显了它在正常发育和疾病过程中的重要性，因此AP-2α (3B5)抗体成为研究人员研究这些关键生物途径的宝贵工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

AP-2α 抗体 (3B5) 参考文献:

  1. 6p24-p25近FKHL7基因座的间隙缺失，包括影响前眼房发育的AP-2alpha。  |  Davies, AF., et al. 1999. J Med Genet. 36: 708-10. PMID: 10507730
  2. AP-2 alpha 和 mu2 亚基的功能分析。  |  Motley, AM., et al. 2006. Mol Biol Cell. 17: 5298-308. PMID: 17035630
  3. AP-2α 可抑制肝癌细胞的生长和迁移。  |  Huang, W., et al. 2016. Int J Oncol. 48: 1125-34. PMID: 26780928
  4. 细胞类型特异性转录因子 AP-2 的克隆和表达，AP-2 能激活诱导增强子元件。  |  Williams, T., et al. 1988. Genes Dev. 2: 1557-69. PMID: 3063603
  5. 通过激活 AP-2α，肝细胞癌细胞的干性得以诱导和维持。  |  Chen, G., et al. 2021. J Exp Clin Cancer Res. 40: 218. PMID: 34193219
  6. AP-2α/AP-2β转录因子是表皮稳态的关键调控因子  |  Zhang, H., et al. 2024. J Invest Dermatol. 144: 1505-1521.e12. PMID: 38237728
  7. 第二个 AP-2 转录因子的克隆和表征：AP-2 beta。  |  Moser, M., et al. 1995. Development. 121: 2779-88. PMID: 7555706
  8. AP-2 基因的另类剪接 mRNA 可编码 AP-2 转录激活的负调控因子。  |  Buettner, R., et al. 1993. Mol Cell Biol. 13: 4174-85. PMID: 8321221
  9. AP-2 转录因子家族两个新成员的人类和小鼠同源物的染色体图谱。  |  Williamson, JA., et al. 1996. Genomics. 35: 262-4. PMID: 8661133
  10. AP-2 蛋白家族调控乳腺癌中 c-erbB-2 的表达。  |  Bosher, JM., et al. 1996. Oncogene. 13: 1701-7. PMID: 8895516
  11. 小鼠胚胎发育过程中 AP-2 alpha 和 AP-2 beta 基因表达的比较分析。  |  Moser, M., et al. 1997. Dev Dyn. 208: 115-24. PMID: 8989526
  12. 鉴定 ERF-1 为 AP2 转录因子家族成员。  |  McPherson, LA., et al. 1997. Proc Natl Acad Sci U S A. 94: 4342-7. PMID: 9113991

订购信息

产品名称产品编号规格价格数量收藏夹

AP-2α 抗体 (3B5)

sc-12726
200 µg/ml
$316.00

AP-2α (3B5): m-IgG Fc BP-HRP 套装

sc-528251
200 µg Ab; 10 µg BP
$354.00

AP-2α (3B5): m-IgGκ BP-HRP 套装

sc-520581
200 µg Ab, 40 µg BP
$354.00

AP-2α 抗体 (3B5) X

sc-12726 X
200 µg/0.1 ml
$316.00

AP-2α 抗体 (3B5) AC

sc-12726 AC
500 µg/ml, 25% agarose
$416.00

AP-2α 抗体 (3B5) HRP

sc-12726 HRP
200 µg/ml
$316.00

AP-2α 抗体 (3B5) FITC

sc-12726 FITC
200 µg/ml
$330.00

AP-2α 抗体 (3B5) PE

sc-12726 PE
200 µg/ml
$343.00

AP-2α 抗体 (3B5) Alexa Fluor® 488

sc-12726 AF488
200 µg/ml
$357.00

AP-2α 抗体 (3B5) Alexa Fluor® 546

sc-12726 AF546
200 µg/ml
$357.00

AP-2α 抗体 (3B5) Alexa Fluor® 594

sc-12726 AF594
200 µg/ml
$357.00

AP-2α 抗体 (3B5) Alexa Fluor® 647

sc-12726 AF647
200 µg/ml
$357.00

AP-2α 抗体 (3B5) Alexa Fluor® 680

sc-12726 AF680
200 µg/ml
$357.00

AP-2α 抗体 (3B5) Alexa Fluor® 790

sc-12726 AF790
200 µg/ml
$357.00

Do you have the protocol to use your agarose beads for IP?

Asked by: AABS
Thank you for your question. Here is our protocol: https://www.scbt.com/resources/protocols/immunoprecipitation-western-blots
Answered by: BlakeJ
Date published: 2023-07-25

Which method of Antigen retrieval is the most suitable for this antibody when detects the protein on Formalin-fixed paraffin-embedded sample?

Asked by: Praphawi
Thank you for your question. You can find our recommended protocol with instructions for antigen retrieval under this link: https://www.scbt.com/de/resources/protocols/immunoperoxidase-staining The recommended antigen retrieval method is heat treatment with citrate buffer as described.
Answered by: Technical Support Europe
Date published: 2020-10-23

Can this antibody be used to stain mouse tissue without non-specific binding?

Asked by: AbPolly
Thank you for your question. Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal antibody AP-2α Antibody (3B5): sc-12726 is currently available conjugated to HRP, PE, FITC, AF488 and AF647.
Answered by: Technical Support 15
Date published: 2017-01-11

Do you have any KO data or other data to show specificity to AP-2a?

Asked by: AbPolly
Thank you for your question. Yes, we do have Western Blot data with siRNA treated HeLa cells showing knockdown of AP-2a and reduction of signal.
Answered by: Technical Support 15
Date published: 2017-01-11
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Rated 5 out of 5 by from work well for WBI've got clear bands for Western Blots, but weak staining for IHC.
Date published: 2022-10-21
Rated 5 out of 5 by from work well on sectionWork really well with OCT cryosection of mouse embryos in immunofluorescence
Date published: 2018-02-19
Rated 5 out of 5 by from Specific bandsSpecific bands was detected for Mouse vascular endothelial cell lysates.1:500 used ,successfully result.
Date published: 2018-01-27
Rated 5 out of 5 by from Great featuresThank you very much, but i didin't test the antibody yet.
Date published: 2017-11-23
Rated 5 out of 5 by from ExcellentImmunofluorescence (1:400) works very well on frozen section from 4% PFA fixed embryonic mouse brain tissue. 3-5 min retrieval with citric buffer (PH6.0) is necessary.
Date published: 2017-10-03
Rated 4 out of 5 by from work well in westernThe antibody works well in western with a dilution of 1:200
Date published: 2017-09-15
Rated 5 out of 5 by from statisfied with the result.This AP-2α is exellent for IF analysis .1:150 diluted the antibody , shows the target expected staining.I am statisfied with the result.
Date published: 2017-09-03
Rated 5 out of 5 by from Superb !!Outstanding western,IF and IHC results; far better than polyclonal alternatives
Date published: 2017-01-14
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AP-2α 抗体 (3B5) is rated 4.8 out of 5 by 20.
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