Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Antiquitin CRISPR/Cas9 KO Plasmid (h): sc-417902
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Antiquitin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Antiquitin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Antiquitin Antibody (A-7): sc-514167
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Antiquitin CRISPR/Cas9 KO Plasmid (h) | sc-417902 | 20 µg | $397.00 |
Overview
ALDH7A1 encodes antiquitin, an NAD+-dependent aldehyde dehydrogenase that participates in lysine catabolism by oxidizing α-aminoadipic semialdehyde and related reactive aldehydes. Through detoxification of aldehyde intermediates, antiquitin contributes to cellular redox homeostasis and limits accumulation of electrophilic metabolites that can perturb metabolic and stress-response pathways. Loss-of-function variants are linked to pyridoxine-dependent epilepsy, reflecting disruption of lysine degradation and secondary effects on vitamin B6/PLP availability and neurotransmitter metabolism. ALDH7A1 is therefore a useful target for studying mitochondrial–cytosolic metabolic coupling, aldehyde stress biology, and inborn errors of metabolism in human cell models.
Antiquitin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ALDH7A1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ALDH7A1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ALDH7A1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Antiquitin protein expression.
This CRISPR knockout system enables efficient generation of ALDH7A1-deficient cell models for investigation of Antiquitin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ALDH7A1 exon(s) critical for Antiquitin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ALDH7A1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Antiquitin CRISPR/Cas9 KO Plasmid (h) and Antiquitin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ALDH7A1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Antiquitin HDR Plasmid (h) and Antiquitin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ALDH7A1 homology arms to support homology-directed repair at defined ALDH7A1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.