Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ANG I CRISPR/Cas9 KO Plasmid (h): sc-401766
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ANG I CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ANG I genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ANG I Antibody (C-1): sc-74528
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ANG I CRISPR/Cas9 KO Plasmid (h) | sc-401766 | 20 µg | $397.00 |
Overview
Angiogenin (ANG), also referred to as ANG I, is a secreted member of the RNase A superfamily that regulates angiogenic signaling and stress-adaptive RNA metabolism. ANG can translocate to the nucleus and promote rRNA transcription, supporting ribosome biogenesis and proliferative capacity, and it also cleaves tRNAs to generate stress-induced fragments that modulate translation. Through these activities, ANG integrates extracellular cues with RNA processing programs that influence endothelial cell behavior, inflammation, and cellular survival under stress. Dysregulated ANG expression or activity has been associated with pathological vascular remodeling and neurodegenerative phenotypes, making it a useful node for mechanistic studies in angiogenesis and RNA stress pathways.
ANG I CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ANG gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ANG together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ANG open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ANG I protein expression.
This CRISPR knockout system enables efficient generation of ANG-deficient cell models for investigation of ANG I signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ANG exon(s) critical for ANG I function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ANG genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ANG I CRISPR/Cas9 KO Plasmid (h) and ANG I CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ANG locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ANG I HDR Plasmid (h) and ANG I HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ANG homology arms to support homology-directed repair at defined ANG target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.