Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
AMPKβ2 CRISPR/Cas9 KO Plasmid (h): sc-403537
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- AMPKβ2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the AMPKβ2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: AMPKβ2 Antibody (C-7): sc-376752
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AMPKβ2 CRISPR/Cas9 KO Plasmid (h) | sc-403537 | 20 µg | $397.00 |
Overview
PRKAB2 encodes the β2 regulatory subunit of AMP-activated protein kinase (AMPK), a heterotrimeric serine/threonine kinase that senses cellular energy status through AMP/ADP-to-ATP ratios. AMPKβ2 contributes to complex assembly and localization and links upstream activation to phosphorylation cascades that restrain anabolic programs while promoting catabolic pathways, including glucose uptake, fatty acid oxidation, and autophagy via cross-talk with mTOR and related nutrient-sensing networks. Through these functions, AMPKβ2 is relevant to studies of metabolic stress adaptation, mitochondrial homeostasis, and cell growth control. Altered AMPK signaling has been associated with metabolic disorders and cancer biology, making PRKAB2 a useful node for probing energy-dependent regulation of proliferation and survival pathways.
AMPKβ2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PRKAB2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PRKAB2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PRKAB2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish AMPKβ2 protein expression.
This CRISPR knockout system enables efficient generation of PRKAB2-deficient cell models for investigation of AMPKβ2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PRKAB2 exon(s) critical for AMPKβ2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PRKAB2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by AMPKβ2 CRISPR/Cas9 KO Plasmid (h) and AMPKβ2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PRKAB2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by AMPKβ2 HDR Plasmid (h) and AMPKβ2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PRKAB2 homology arms to support homology-directed repair at defined PRKAB2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.