Date published: 2025-9-7

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AKAP 220 抗体 (13): sc-135825

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说明书
  • AKAP 220 抗体 13 是小鼠单克隆 IgG1AKAP 220抗体,规格为200 µg/ml
  • 免疫rat物种的AKAP 220的氨基酸11-173
  • 推荐用于 mouse 和 rat 来源的AKAP 220 WB, IP 和 IF检测; 不建议用于IP
  • 关于如何获取AKAP 220 (13): sc-135825免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是AKAP 220 Antibody (13) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与AKAP 220 Antibody (13) 打包提供(请参阅下面的订购信息)。

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    関連項目

    AKAP 220 抗体 (13) 是一种 IgG1 κ 小鼠单克隆 AKAP 220 抗体(也称 AKAP11 抗体),可通过 WB 和 IF 检测小鼠和大鼠 AKAP 220 蛋白。AKAP 220 抗体 (13) 可作为非结合型抗 AKAP 220 抗体提供。II 型 cAMP 蛋白激酶(PKA)是一种多功能激酶,有多种底物。人们认为,PKA 信号传导的特异性是由激酶在细胞内特定部位的分区介导的。为了维持这种特异性定位,PKA 的 R 亚基(RII)与特定的 RII 锚定蛋白相互作用。这个蛋白家族被命名为 A 激酶锚定蛋白(AKAP)。该家族成员包括 MAP2(微管相关蛋白 2)、神经表达的 AKAP 79 和 AKAP 150 以及 DNA 结合的 AKAP 95,它们显示出不同的组织特异性和定位。AKAP 220 可能通过靶向 II 型 PKA 在 cAMP 反应性过氧物酶体事件中发挥作用。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    AKAP 220 抗体 (13) 参考文献:

    1. 通过 A 激酶锚定蛋白将 cAMP 依赖性蛋白激酶定域到突触后密度。AKAP 79的特征。  |  Carr, DW., et al. 1992. J Biol Chem. 267: 16816-23. PMID: 1512224
    2. II 型调节亚基二聚化决定了 cAMP 依赖性蛋白激酶的亚细胞定域。  |  Scott, JD., et al. 1990. J Biol Chem. 265: 21561-6. PMID: 2147685
    3. 蛋白激酶 A 和蛋白磷酸酶 2B 与共同锚定蛋白的关联  |  Coghlan, VM., et al. 1995. Science. 267: 108-11. PMID: 7528941
    4. A 激酶锚定蛋白:选择性激活 cAMP 响应事件的关键?  |  Coghlan, VM., et al. 1993. Mol Cell Biochem. 127-128: 309-19. PMID: 7935359
    5. 与 II 型 cAMP 依赖性蛋白激酶调节亚基缔合作用的核蛋白 AKAP 95 的克隆和特征描述。  |  Coghlan, VM., et al. 1994. J Biol Chem. 269: 7658-65. PMID: 8125992
    6. 一种新型 A 激酶锚定蛋白的克隆和表征。AKAP 220,与睾丸过氧化物酶体的缔合作用。  |  Lester, LB., et al. 1996. J Biol Chem. 271: 9460-5. PMID: 8621616

    订购信息

    产品名称产品编号规格价格数量收藏夹

    AKAP 220 抗体 (13)

    sc-135825
    200 µg/ml
    $316.00

    AKAP 220 (13): m-IgG Fc BP-HRP 套装

    sc-537371
    200 µg Ab; 10 µg BP
    $354.00

    AKAP 220 (13): m-IgGκ BP-HRP 套装

    sc-534621
    200 µg Ab; 40 µg BP
    $354.00

    AKAP 220 (13): m-IgG1 BP-HRP 套装

    sc-545158
    200 µg Ab; 20 µg BP
    $354.00

    I would like to know the (1) protein load (ug), (2) gel %, (3) running (mA and time) and transfer condition (mA, time) for the positive control, rat testis tissue extract for the western blot of AKAP 220 expression in rat testis tissue extract. Thanks.

    Asked by: Vicw
    Thank you for your question. We loaded 50 ug rat testis tissue extract, on an 8% gel. We transfer proteins onto a PVDF membrane overnight at 0.1 Amp.
    Answered by: Technical Support
    Date published: 2019-01-26

    What is the recommended fixation method for immunofluorescence staining with AKAP 220 (13): sc-135825 monoclonal antibody?

    Asked by: Dr Ninau Qelp
    Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10ºC methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
    Answered by: Technical Support
    Date published: 2017-03-24
    • y_2025, m_9, d_6, h_5CST
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    Rated 5 out of 5 by from Superb immunofluorescence nuclear and cytoplasmicSuperb immunofluorescence nuclear and cytoplasmic staining in MCF7 cells. -SCBT QC
    Date published: 2015-04-09
    Rated 5 out of 5 by from Positive signal in WB in rat testis tissue extractPositive signal in WB in rat testis tissue extract. -SCBT QC
    Date published: 2015-03-06
    Rated 5 out of 5 by from Robust signal in WB in KNRK whole cell lysateRobust signal in WB in KNRK whole cell lysate and rat testis tissue extract. -SCBT QC
    Date published: 2015-02-20
    Rated 5 out of 5 by from Strong band in WB in rat brain tissue extractStrong band in WB in rat brain tissue extract. -SCBT QC
    Date published: 2015-02-07
    Rated 5 out of 5 by from Good specificity in WB in MCF7 whole cell lysateGood specificity in WB in MCF7 whole cell lysate. -SCBT QC
    Date published: 2015-01-09
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    AKAP 220 抗体 (13) is rated 5.0 out of 5 by 5.
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