Date published: 2025-9-9
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AIF Antibody (E-1) - Western Blotting - Image 352401
AIF 抗体 (E-1): sc-13116. CCRF-CEM (A), MOLT-4 (B), C2C12 (C), Sol8 (D), L6 (E) 和 K-562 (F) 全细胞裂解液中 AIF 表达的 Western 印迹分析.
AIF 抗体 (E-1): sc-13116. CCRF-CEM (A), MOLT-4 (B), C2C12 (C), Sol8 (D), L6 (E) 和 K-562 (F) 全细胞裂解液中 AIF 表达的 Western 印迹分析.

AIF 抗体 (E-1): sc-13116

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说明书
  • AIF 抗体 E-1 是小鼠单克隆 IgG2b κ，AIF抗体, 在269篇文献中引用，规格为200 µg/ml
  • 免疫human物种的AIF的氨基酸1-300
  • AIF 抗体 (E-1) 推荐用于 WB, IP, IF, IHC(P), FCM 和 ELISA，检测mouse, rat 和human 来源的 AIF
  • 抗AIF抗体（E-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取AIF (E-1): sc-13116的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 AIF 抗体 (E-1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与AIF 抗体 (E-1) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 AIF 抗体包括 AIFL

AIF抗体（E-1）是一种IgG2b κ型小鼠单克隆AIF抗体（也称为AIFM1抗体），可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学（IHC-P）、流式细胞术（FCM）和酶联免疫吸附测定（ELISA）检测来自小鼠、大鼠和人的AIF蛋白。AIF抗体（E-1）可作为非偶联抗AIF抗体形式，以及多种偶联形式的抗AIF抗体，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®偶联物。凋亡过程中的一个关键事件是线粒体通透性转换孔的开放，该事件受Bcl-2家族蛋白的调节，导致线粒体膜间隙中几种蛋白质的释放。这些蛋白质中有几种参与凋亡，包括细胞色素c、procaspases 2、3和9以及AIF（凋亡诱导因子）。研究表明，AIF可导致DNA断裂和染色质凝聚，并诱导线粒体释放细胞色素c和caspase-9。研究还表明，Bcl-2的过表达可以防止AIF从线粒体中释放，但不能阻止其凋亡活性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

AIF 抗体 (E-1) 参考文献:

  1. 凋亡诱导因子（AIF）：线粒体释放的一种新型不依赖于树突酶的死亡效应因子。  |  Candé, C., et al. 2002. Biochimie. 84: 215-22. PMID: 12022952
  2. Bcl-2 家族蛋白和线粒体在细胞凋亡中的作用。  |  Kuwana, T. and Newmeyer, DD. 2003. Curr Opin Cell Biol. 15: 691-9. PMID: 14644193
  3. 人类 RNA/DNA 螺旋酶 SUV3 的下调可通过依赖于 Caspase 和 AIF 的途径诱导细胞凋亡。  |  Szczesny, RJ., et al. 2007. Biol Cell. 99: 323-32. PMID: 17352692
  4. 人骨肉瘤细胞在黄酮类化合物作用下不依赖于Caspase的死亡是由p53介导的线粒体压力以及AIF和内切酶G的核转位驱动的。  |  Kook, SH., et al. 2007. Apoptosis. 12: 1289-98. PMID: 17356895
  5. 生理和疾病中的细胞凋亡诱导因子（AIF）：一个忏悔的天生杀手的故事。  |  Bano, D. and Prehn, JHM. 2018. EBioMedicine. 30: 29-37. PMID: 29605508
  6. 线粒体对细胞核凋亡的控制。  |  Zamzami, N., et al. 1996. J Exp Med. 183: 1533-44. PMID: 8666911
  7. Bcl-2 可抑制线粒体释放一种致凋亡蛋白酶。  |  Susin, SA., et al. 1996. J Exp Med. 184: 1331-41. PMID: 8879205
  8. 线粒体释放细胞色素 c：Bcl-2 调控细胞凋亡的主要场所。  |  Kluck, RM., et al. 1997. Science. 275: 1132-6. PMID: 9027315
  9. Caspase-3前体在细胞膜和线粒体中的分布：对凋亡信号转导的影响。  |  Mancini, M., et al. 1998. J Cell Biol. 140: 1485-95. PMID: 9508780
  10. 线粒体和细胞凋亡  |  Green, DR. and Reed, JC. 1998. Science. 281: 1309-12. PMID: 9721092
  11. 线粒体在细胞凋亡过程中释放 caspase-2 和 -9。  |  Susin, SA., et al. 1999. J Exp Med. 189: 381-94. PMID: 9892620
  12. 线粒体凋亡诱导因子的分子特征。  |  Susin, SA., et al. 1999. Nature. 397: 441-6. PMID: 9989411

订购信息

产品名称产品编号规格价格数量收藏夹

AIF 抗体 (E-1)

sc-13116
200 µg/ml
$316.00

AIF (E-1): m-IgG Fc BP-HRP 套装

sc-528260
200 µg Ab; 10 µg BP
$354.00

AIF (E-1): m-IgGκ BP-HRP 套装

sc-520593
200 µg Ab, 40 µg BP
$354.00

AIF (E-1): m-IgG2b BP-HRP 套装

sc-550014
200 µg Ab; 10 µg BP
$354.00

AIF 抗体 (E-1) AC

sc-13116 AC
500 µg/ml, 25% agarose
$416.00

AIF 抗体 (E-1) HRP

sc-13116 HRP
200 µg/ml
$316.00

AIF 抗体 (E-1) FITC

sc-13116 FITC
200 µg/ml
$330.00

AIF 抗体 (E-1) PE

sc-13116 PE
200 µg/ml
$343.00

AIF 抗体 (E-1) Alexa Fluor® 488

sc-13116 AF488
200 µg/ml
$357.00

AIF 抗体 (E-1) Alexa Fluor® 546

sc-13116 AF546
200 µg/ml
$357.00

AIF 抗体 (E-1) Alexa Fluor® 594

sc-13116 AF594
200 µg/ml
$357.00

AIF 抗体 (E-1) Alexa Fluor® 647

sc-13116 AF647
200 µg/ml
$357.00

AIF 抗体 (E-1) Alexa Fluor® 680

sc-13116 AF680
200 µg/ml
$357.00

AIF 抗体 (E-1) Alexa Fluor® 790

sc-13116 AF790
200 µg/ml
$357.00

Why AIF showing two bands

Asked by: Anu97
Thank you for your question. Please call or email technical service . (800)-457-3801 or scbt@scbt.com
Answered by: BlakeJ
Date published: 2023-06-21

I did IF staining (1:250) on fresh frozen mouse tumour section together with anti mouse conjugated to 647 as secondary antibody. However, there is very high signal on my negative stain where i only stain with the secondary antibody. Pls advise

Asked by: OWEJ
Thank you for your product review. We are sorry to hear that this antibody did not perform as expected. We want you to be successful in your research, so please contact our Technical Service Department by phone, e-mail or live chat.
Answered by: Technical Service
Date published: 2022-08-04

Can AIF Antibody (E-1): sc-13116 be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, AIF Antibody (E-1): sc-13116 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 15
Date published: 2017-01-09
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Rated 5 out of 5 by from western blotThe antibody can gave a nice clean band. It is a good antibody.
Date published: 2023-03-10
Rated 5 out of 5 by from 免疫印迹申请了小包装，1：300稀释的，免疫印迹实验，条带比较清晰，还不错啊！推荐一下吧..........
Date published: 2020-12-20
Rated 5 out of 5 by from LOVED ITIT is excellent. Signal so strong, recommend it. Use it for Western and it lasted a year.
Date published: 2020-07-02
Rated 5 out of 5 by from AIF antibodyThis antibody works really well and clean in muscle cells.
Date published: 2019-09-10
Rated 5 out of 5 by from Great antibodyThe antibody worked really nice for western blot in NPC-TW 076 cells.
Date published: 2019-07-18
Rated 5 out of 5 by from antibodyantibody works really well in WB of mouse myoblasts.
Date published: 2019-05-29
Rated 5 out of 5 by from Great antibodyThe antibody worked really nice for endogenous detection by western blot in HEK 293T cells.
Date published: 2018-10-25
Rated 5 out of 5 by from great antibodyThis antibody is clean and at predicted MW. Been using for a while.
Date published: 2017-11-27
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AIF 抗体 (E-1) is rated 4.7 out of 5 by 33.
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