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Ah Receptor Antibody (L-15) - Western Blotting - Image 126764
Ah 受体抗体 (L-15): sc-101104. 未转染 293: sc-110760 (A), 转染小鼠 Ah 受体的 293: sc-178266 (B) 和 PC-3 (C) 全细胞裂解液中 Ah 受体表达的 Western 印迹分析.
Ah 受体抗体 (L-15): sc-101104. 未转染 293: sc-110760 (A), 转染小鼠 Ah 受体的 293: sc-178266 (B) 和 PC-3 (C) 全细胞裂解液中 Ah 受体表达的 Western 印迹分析.

Ah Receptor 抗体 (L-15): sc-101104

4.0(3)
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说明书
  • Ah Receptor抗体(L-15)是小鼠单克隆IgG1 κ, 在5篇文献中引用，规格为100 µg/ml
  • 免疫human物种的重组Ah Receptor
  • 推荐用于 mouse, rat 和 human 来源的Ah Receptor WB, IP, IF 和 ELISA检测
  • 看 Ah Receptor (A-3): sc-133088，可查看有标记的 Ah Receptor 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 目前，我们还没有完成Ah Receptor 抗体 (L-15)的首选二抗检测试剂的鉴定。这项工作正在进行中。
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2,3,7,8-四氯二苯并二噁英（TCDD）是一系列有毒卤代芳香族化合物的原型，被认为会引起不利的生殖、免疫和代谢影响。 TCDD的许多生物反应是通过配体与芳香烃（Ah）受体结合来介导的，Ah受体也称为AhR。 Ah受体是一种配体依赖性转录因子，与特定的DNA序列相互作用，称为外源性应答元件（XRE），位于TCDD诱导基因的上游。与配体结合后，Ah受体与Ah受体核转运子（Arnt）结合，复合物从细胞质转移到细胞核。 Arnt是Ah受体与XRE结合所必需的。 Ah受体和Arnt是转录因子家族的成员，包含一个基本螺旋-环-螺旋基序和一个常见的"PAS"基序。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Ah Receptor 抗体 (L-15) 参考文献:

  1. Ah受体信号与多种发育途径的相互作用。  |  Puga, A., et al. 2005. Biochem Pharmacol. 69: 199-207. PMID: 15627472
  2. 干细胞周期、调控和静止中的Ah受体。  |  Gasiewicz, TA., et al. 2014. Ann N Y Acad Sci. 1310: 44-50. PMID: 24495120
  3. 阐明二噁英介导的氯痤疮和 Ah 受体功能。  |  Bock, KW. 2016. Biochem Pharmacol. 112: 1-5. PMID: 26801687
  4. Ah 受体配体及其对肠道恢复能力的影响：结构-活性效应。  |  Safe, S., et al. 2020. Crit Rev Toxicol. 50: 463-473. PMID: 32597352
  5. 肠道微生物群衍生的色氨酸代谢物可预测 Ah 受体的活性。  |  Dong, F., et al. 2020. Gut Microbes. 12: 1-24. PMID: 32783770
  6. 了解其生理功能时，如何理解阿受体配体的特异性变得非常重要。  |  Murray, IA. and Perdew, GH. 2020. Int J Mol Sci. 21: PMID: 33348604
  7. 复杂的化学信号通过肠道-肺轴线决定Ah受体的激活。  |  Dong, F., et al. 2023. FASEB J. 37: e23010. PMID: 37272852
  8. 从丹参中发现天然Ah受体拮抗剂，并合成类似物用于肿瘤免疫治疗。  |  Li, Z., et al. 2024. J Med Chem. 67: 1243-1261. PMID: 38176026
  9. AH受体：遗传学、结构和功能。  |  Swanson, HI. and Bradfield, CA. 1993. Pharmacogenetics. 3: 213-30. PMID: 8287061
  10. Ah受体信号通路。  |  Schmidt, JV. and Bradfield, CA. 1996. Annu Rev Cell Dev Biol. 12: 55-89. PMID: 8970722

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产品名称产品编号规格价格数量收藏夹

Ah Receptor 抗体 (L-15)

sc-101104
100 µg/ml
$339.00

I will use Ah Receptor (L-15) : sc-101104 for WB in Human fibroblast cells, what dilution of this antibody should I use and what is the best, BSA or milk for blocking ? What kind of membrane should be the best to reveal the Ah Receptor ?

Asked by: Cells
Thank you for your question. The recommended starting dilution for this antibody is 1:200, with a dilution range of 1:100-1:1000. We recommend using UltraCruz® Blocking Reagent: sc-516214 for blocking, which is also the reagent that is used to establish our antibodies before they are released. It is also possible to block with 5 % BSA TBST (if binding is promiscuous) or 5 % non-fatty milk TBST (if binding is rather weak). For further information, please contact our technical service.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

I am using Ah Receptor (L-15): sc-101104 for immunofluorescence, what dilution of this antibody should I use?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by TS from Good immunofluorescence nuclear stainingGood immunofluorescence nuclear staining in paraformaldehyde-fixed HeLa cells. -SCBT QC
Date published: 2015-05-20
Rated 4 out of 5 by SF from Great WB signal achieved in human Ah ReceptorGreat WB signal achieved in human Ah Receptor transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-04-20
Rated 4 out of 5 by ET from Very precise signal obtained in WB with COLOVery precise signal obtained in WB with COLO 320 HSR whole cell lysate. -SCBT QC
Date published: 2015-04-14
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Ah Receptor 抗体 (L-15) is rated 4.0 out of 5 by 3.
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