Date published: 2025-9-6
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Ah Receptor Antibody (A-3) - Western Blotting - Image 372684
Ah 受体抗体 (A-3): sc-133088. PC-3 (A), Hep G2 (B), MDA-MB-435S (C) 和 A-431 (D) 全细胞裂解液中 Ah 受体表达的近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.
Ah 受体抗体 (A-3): sc-133088. PC-3 (A), Hep G2 (B), MDA-MB-435S (C) 和 A-431 (D) 全细胞裂解液中 Ah 受体表达的近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.

Ah Receptor 抗体 (A-3): sc-133088

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寫評論提問

说明书
  • Ah Receptor 抗体 A-3 是小鼠单克隆 IgG2b κ，Ah Receptor抗体, 在109篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Ah Receptor的氨基酸637-848
  • Ah Receptor 抗体 (A-3) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Ah Receptor
  • 抗Ah Receptor抗体（A-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-133088 X, 200 µg/0.1 ml)
  • 关于如何获取Ah Receptor (A-3): sc-133088的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 Ah Receptor 抗体 (A-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Ah Receptor 抗体 (A-3) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

Ah受体抗体（A-3）是一种IgG2b κ小鼠单克隆Ah受体抗体（也称为芳香烃受体抗体或AHR抗体），可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人源的芳香烃受体蛋白。 Ah受体抗体（A-3）有非偶联的抗芳香烃受体抗体形式，以及多种偶联形式的抗Ah受体抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。 2,3,7,8-四氯二苯并-p-二恶英（TCDD）是一类有毒卤代芳香族化合物的原型，被认为会引起不良的生殖、免疫和代谢效应。 TCDD的许多生物反应是通过与芳香烃（Ah）受体（也称为AhR）结合的配体介导的。 Ah受体是一种配体依赖性转录因子，与特定的DNA序列相互作用，称为外源性应答元件（XRE），位于TCDD诱导基因的上游。与配体结合后，Ah受体与Ah受体核转运子（Arnt）结合，复合物从细胞质转移到细胞核。 Arnt是Ah受体与XRE结合所必需的。 Ah受体和Arnt是转录因子家族的成员，包含一个基本螺旋-环-螺旋基序和一个常见的"PAS"基序。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Ah Receptor 抗体 (A-3) 参考文献:

  1. 确定 Ah 受体核转运蛋白（Arnt）是 Ah 受体 DNA 结合形式的组成部分。  |  Reyes, H., et al. 1992. Science. 256: 1193-5. PMID: 1317062
  2. Ah 受体通过 NC-XRE 驱动的 p21Cip1 表达介导的肝脏再生抑制。  |  Jackson, DP., et al. 2014. Mol Pharmacol. 85: 533-41. PMID: 24431146
  3. 抑制Ah受体（AhR）可增加TNBC细胞的侵袭性，而11-Cl-BBQ激活的AhR可抑制其生长。  |  Elson, DJ., et al. 2023. Biochem Pharmacol. 215: 115706. PMID: 37506922
  4. 免疫细胞内源性Ah受体促进小肠中抗菌蛋白REG3G的表达。  |  Chakraborty, D., et al. 2024. FASEB J. 38: e23471. PMID: 38358358
  5. Ah 受体：2,3,7,8-四氯二苯并对二恶英（TCDD）及相关化合物毒性的媒介。  |  Okey, AB., et al. 1994. Toxicol Lett. 70: 1-22. PMID: 8310450
  6. 异源二聚体芳基烃受体复合物亚基 DNA 结合域的功能表征，归因于新的和典型的基本螺旋-环-螺旋蛋白-DNA 相互作用。  |  Bacsi, SG. and Hankinson, O. 1996. J Biol Chem. 271: 8843-50. PMID: 8621524
  7. 一种新型碱性螺旋-环-螺旋/PAS因子（Arnt2）的cDNA克隆和组织特异性表达，其序列与芳基烃受体核转运体（Arnt）非常相似。  |  Hirose, K., et al. 1996. Mol Cell Biol. 16: 1706-13. PMID: 8657146
  8. 人芳基烃受体核转运体/缺氧诱导因子 1beta 的核定位信号是核孔靶向复合体的两个组成部分所识别的一种新型双端类型。  |  Eguchi, H., et al. 1997. J Biol Chem. 272: 17640-7. PMID: 9211913
  9. 两种类胡萝卜素（角黄素和β-apo-8'-胡萝卜醛）诱导的Ah受体依赖性CYP1A，与TCDD结合位点无亲和力。  |  Gradelet, S., et al. 1997. Biochem Pharmacol. 54: 307-15. PMID: 9271336
  10. 两种脊椎动物芳基烃（二恶英）受体（AHR1 和 AHR2）以及 PAS 家族的分子进化。  |  Hahn, ME., et al. 1997. Proc Natl Acad Sci U S A. 94: 13743-8. PMID: 9391097
  11. 培养的哺乳动物细胞系中的芳基烃受体（AHR）发生突变，确定了影响 DNA 结合的 AHR 新区域。  |  Sun, W., et al. 1997. J Biol Chem. 272: 31845-54. PMID: 9395531
  12. 人类对二恶英反应的动物模型。  |  Grassman, JA., et al. 1998. Environ Health Perspect. 106 Suppl 2: 761-75. PMID: 9599728

订购信息

产品名称产品编号规格价格数量收藏夹

Ah Receptor 抗体 (A-3)

sc-133088
200 µg/ml
$316.00

Ah Receptor (A-3): m-IgGκ BP-HRP 套装

sc-521229
200 µg Ab, 40 µg BP
$354.00

Ah Receptor (A-3): m-IgG2b BP-HRP 套装

sc-548874
200 µg Ab; 10 µg BP
$354.00

Ah Receptor 抗体 (A-3) X

sc-133088 X
200 µg/0.1 ml
$316.00

Ah Receptor 抗体 (A-3) AC

sc-133088 AC
500 µg/ml, 25% agarose
$416.00

Ah Receptor 抗体 (A-3) HRP

sc-133088 HRP
200 µg/ml
$316.00

Ah Receptor 抗体 (A-3) FITC

sc-133088 FITC
200 µg/ml
$330.00

Ah Receptor 抗体 (A-3) PE

sc-133088 PE
200 µg/ml
$343.00

Ah Receptor 抗体 (A-3) Alexa Fluor® 488

sc-133088 AF488
200 µg/ml
$357.00

Ah Receptor 抗体 (A-3) Alexa Fluor® 546

sc-133088 AF546
200 µg/ml
$357.00

Ah Receptor 抗体 (A-3) Alexa Fluor® 594

sc-133088 AF594
200 µg/ml
$357.00

Ah Receptor 抗体 (A-3) Alexa Fluor® 647

sc-133088 AF647
200 µg/ml
$357.00

Ah Receptor 抗体 (A-3) Alexa Fluor® 680

sc-133088 AF680
200 µg/ml
$357.00

Ah Receptor 抗体 (A-3) Alexa Fluor® 790

sc-133088 AF790
200 µg/ml
$357.00

请问做ChIP-qPCR推荐使用多少抗体？

Asked by: polaris
感谢您的提问。sc-133088 X才可以支持用于ChIP，以下是ChIP的实验流程，请您参考。 https://www.scbt.com/zh/resources/protocols/chromatin-immunoprecipitation-assays Thank you for your question. sc-133088 X is the only one that can be supported for use in ChIP, and the following is the experimental procedure for ChIP. https://www.scbt.com/zh/resources/protocols/chromatin-immunoprecipitation-assays
Answered by: Jiajun Z
Date published: 2024-04-17

请问这是兔抗还是鼠抗

Asked by: ting
感谢您的提问。Ah Receptor 抗体 (A-3): sc-133088是小鼠来源的单克隆抗体。 Thank you for your question. Ah Receptor antibody (A-3): sc-133088 is mouse monoclonal antibody.
Answered by: Technical Service SH
Date published: 2024-03-25

我从您们的网站上看到之前的Ah receptor antibody可以应用于斑马鱼，但是目前已经停产了，请问目前的单抗替代品Ah receptor antibody(A-3）还适用于斑马鱼吗？

Asked by: shmilym1126
感谢您的提问。关于此问题的详细细节，欢迎通过：电话(+86) 021-60936350、邮箱asia@scbio.cn、或选择网页上Live Chat中的“中文支持”，联系我们的技术支持部门。 Thank you for your inquiry. For more detailed information about this question, please contact our Asian Technical Service team at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Technical Support 15
Date published: 2018-04-20

Should I use a secondary antibody for western blotting, or do you recommend something else?

Asked by: Anna99
Instead of using a traditional secondary, we suggest using our Mouse IgG kappa Binding Protein HRP, sc-516102. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 9
Date published: 2017-01-09
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Rated 1 out of 5 by from The antibody did not work properlyWe tested the antibody in western blotting on protein extract from HeLa cells (40 ug). Unfortunately, in our experimental conditions, the antibody seems to not work well. We observed many aspecific bands, with no clear results.
Date published: 2023-11-07
Rated 5 out of 5 by from AHR-sc-A3, AHR-Invitrogen-RPT9-MA1-513, AHR-sc-B11I compared three different antibodies for SCC-25 human cells and this is the result: AHR-sc-A3, AHR-Invitrogen-RPT9-MA1-513, AHR-sc-B11
Date published: 2021-05-28
Rated 4 out of 5 by from Verified in WB. IF. co-IP and ChIP-qPCR2ug of this antibody used for co-IP and 4ug for ChIP-qPCR. When used in WB on beast cancer cell lines, it shows a band at 120kDa which is higher than expected molecular weight.
Date published: 2020-02-17
Rated 5 out of 5 by from Very good ab for WBUsed it for WB, IF, IHC on human cells and tissues. Antibody stains single band of expected size (between 110 and 130 kDa) on WB without background bands typical for rabbit polyclonal anti-AhR, even at high exposure. IF, IHC - produces strong cytoplasmic staining (probably, as you would expect in normal conditions).
Date published: 2019-04-05
Rated 5 out of 5 by from Excellent specific signalI got two free samples for Anti-AhR (sc-133088 and sc-74571), and tested WB on some breast cancer cell lines. Both of them work well and I got similar specific signal. I strongly suggested them.
Date published: 2018-10-31
Rated 5 out of 5 by from Works for WBGave a nice clear band when used at 1:200 dilution for mouse tissue lysates.
Date published: 2018-03-23
Rated 5 out of 5 by from Good antibody!1:100 diluted for wb of rat protein which extracted from h9c2 cells,the results were satisfied!
Date published: 2017-12-31
Rated 4 out of 5 by from Frozen mouse tissueIt shows nuclear staining in the wild type mouse stomach, which disappears in the disease model stomach.
Date published: 2017-11-27
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Ah Receptor 抗体 (A-3) is rated 4.4 out of 5 by 21.
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