Date published: 2026-10-19

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Ah Receptor 抗体 (B-11): sc-74571

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说明书
  • Ah Receptor抗体(B-11)是小鼠单克隆IgG2a κ, 在12篇文献中引用,规格为200 µg/ml
  • 免疫human物种的Ah Receptor的氨基酸637-848
  • 抗-Ah Receptor 抗体 (B-11) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Ah Receptor
  • 抗Ah Receptor抗体(B-11)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-74571 X, 200 µg/0.1 ml)
  • m-IgG Fc BP-HRP是Ah Receptor Antibody (B-11) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。该试剂现与Ah Receptor Antibody (B-11) 搭配使用(请参阅下面的订购信息)。
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関連項目

Ah受体抗体(B-11)是一种IgG2a κ小鼠单克隆Ah受体抗体(也称为Ah受体抗体或芳香烃受体抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源的Ah受体。 Ah受体抗体(B-11)可以以非偶联的抗Ah受体抗体形式提供,以及多种偶联形式的抗Ah受体抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。 2,3,7,8-四氯二苯并-p-二恶英(TCDD)是一类有毒卤代芳香族化合物的原型,被认为会引起不良的生殖、免疫和代谢效应。 TCDD的许多生物反应是通过与芳香烃(Ah)受体(也称为AhR)结合的配体介导的。 Ah受体是一种配体依赖性转录因子,与特定的DNA序列相互作用,称为外源性应答元件(XRE),位于TCDD诱导基因的上游。与配体结合后,Ah受体与Ah受体核转运子(Arnt)结合,复合物从细胞质转移到细胞核。 Arnt是Ah受体与XRE结合所必需的。 Ah受体和Arnt是转录因子家族的成员,包含一个基本螺旋-环-螺旋基序和一个常见的"PAS"基序。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Ah Receptor 抗体 (B-11) 参考文献:

  1. Ah受体的分子生物学及其在癌变中的作用。  |  Safe, S. 2001. Toxicol Lett. 120: 1-7. PMID: 11323156
  2. AH受体蛋白下调(降解)的机制及其对AH受体介导的基因调控的影响。  |  Pollenz, RS. 2002. Chem Biol Interact. 141: 41-61. PMID: 12213384
  3. Ah受体和TCDD介导的肝肿瘤促进:逃避生长停滞和细胞凋亡的细胞克隆选择和扩增。  |  Bock, KW. and Köhle, C. 2005. Biochem Pharmacol. 69: 1403-8. PMID: 15857604
  4. 人类药物代谢酶和结合转运体的协调调节,受阿受体、孕烷X受体和组成雄烷受体的影响。  |  Köhle, C. and Bock, KW. 2009. Biochem Pharmacol. 77: 689-99. PMID: 18606396
  5. Ah受体对胆红素稳态及其抗氧化和抗动脉粥样硬化功能的贡献。  |  Bock, KW. and Köhle, C. 2010. Biol Chem. 391: 645-53. PMID: 20370320
  6. 类人进化过程中不同的 Ah 受体配体选择性  |  Hubbard, TD., et al. 2016. Mol Biol Evol. 33: 2648-58. PMID: 27486223
  7. 通过条件修饰和使用哺乳动物修饰剂,增强酵母中Ah受体系统的敏感性。  |  Vazquez-Rivera, E., et al. 2022. Toxicol Rep. 9: 513-520. PMID: 35356645
  8. 内源性色氨酸衍生的 Ah 受体配体与依赖 CYP1A1/1B1 的负反馈相分离。  |  Dong, F., et al. 2023. Int J Tryptophan Res. 16: 11786469231182508. PMID: 37434789
  9. 循环宿主和微生物色氨酸代谢产物对Ah受体活化的贡献。  |  Morgan, EW., et al. 2023. Int J Tryptophan Res. 16: 11786469231182510. PMID: 37441265
  10. Ah受体和二噁英诱导[Ah]基因电池在毒性、癌症和信号转导中的作用。  |  Nebert, DW., et al. 1993. Ann N Y Acad Sci. 685: 624-40. PMID: 8395783

订购信息

产品名称产品编号规格价格数量收藏夹

Ah Receptor 抗体 (B-11)

sc-74571
200 µg/ml
$322.00

Ah Receptor (B-11): m-IgG Fc BP-HRP 套装

sc-525732
200 µg Ab; 10 µg BP
$361.00

Ah Receptor 抗体 (B-11) X

sc-74571 X
200 µg/0.1 ml
$322.00

Ah Receptor 抗体 (B-11) AC

sc-74571 AC
500 µg/ml, 25% agarose
$424.00

Ah Receptor 抗体 (B-11) HRP

sc-74571 HRP
200 µg/ml
$322.00

Ah Receptor 抗体 (B-11) FITC

sc-74571 FITC
200 µg/ml
$336.00

Ah Receptor 抗体 (B-11) PE

sc-74571 PE
200 µg/ml
$349.00

Ah Receptor 抗体 (B-11) Alexa Fluor® 488

sc-74571 AF488
200 µg/ml
$364.00

Ah Receptor 抗体 (B-11) Alexa Fluor® 546

sc-74571 AF546
200 µg/ml
$364.00

Ah Receptor 抗体 (B-11) Alexa Fluor® 594

sc-74571 AF594
200 µg/ml
$364.00

Ah Receptor 抗体 (B-11) Alexa Fluor® 647

sc-74571 AF647
200 µg/ml
$364.00

Ah Receptor 抗体 (B-11) Alexa Fluor® 680

sc-74571 AF680
200 µg/ml
$364.00

Ah Receptor 抗体 (B-11) Alexa Fluor® 790

sc-74571 AF790
200 µg/ml
$364.00

When should the TransCruz reagent sc-74571 X be used instead of the standard form sc-74571?

Asked by: Trav11
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by Simona PG from provato in IF su sezione paraffinataSembra funzionare abbastanza su sezioni di colon paraffinate, ma bisogna provare ulteriori diluizioni per ottenere uno staining ottimale
Date published: 2024-02-15
Rated 1 out of 5 by Elhamdianati from Not idealOur results wasn't ideal in IF. WB using 2ug in two breast cancer cell lines
Date published: 2020-02-17
Rated 3 out of 5 by MuGu from Works for WB mouse tissueWorks for WB at 1:200 for Mouse tissue samples. However, also has non-specific binding.
Date published: 2018-03-22
Rated 3 out of 5 by Jackie87 from compare with sc-133088I received two free samples of anti-AhR antibodies. This antibody works not as good as sc-133088 with my samples. I wanted to detect the endogenous protein expression in 3T3-L1 cells. For this antibody, I also use 1:1000 dilution, incubate at 4 degree for 24-48h and secondary antibody (mouse) at 1:5000, 4 degree for 5-7h. I did not get clean bands as sc-133088. However, this antibody works well with HEK293T cell lysate.
Date published: 2017-09-25
Rated 5 out of 5 by snowy from positive stainingThe positive staining can be seen in the cytoplasm of the hepatocytes.
Date published: 2017-02-05
Rated 5 out of 5 by Dr Ninau Qelp from Great antibodyAh Receptor (B-11) worked great for Western blot (WB), strong band at expected molecular weight in PC-3 Cell Lysate: sc-2220.
Date published: 2017-02-03
Rated 5 out of 5 by LR from Great WB signal obtained using this antibodyGreat WB signal obtained using this antibody against HeLa whole cell lysate. -SCBT QC
Date published: 2015-05-12
Rated 5 out of 5 by SM from Publishable Western blot results using humanPublishable Western blot results using human MCF10A cells. -SCBT Publication Review
Date published: 2015-04-30
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Ah Receptor 抗体 (B-11) is rated 3.9 out of 5 by 11.
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