Date published: 2025-9-7

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ADAR3 抗体 (3.591): sc-73410

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说明书
  • ADAR3 抗体 3.591 是小鼠单克隆 IgG κ,ADAR3抗体, 在6篇文献中引用,规格为200 µg/ml
  • 与human的ADAR3的N端氨基酸2-101对应的合成肽的免疫原性
  • ADAR3 抗体 (3.591) 推荐用于 WB 和 IP,检测mouse, rat 和human 来源的 native and recombinant ADAR3; 不会与 other members of the ADAR family产生交联反应
  • 抗ADAR3抗体(3.591)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取ADAR3 (3.591): sc-73410免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP是ADAR3 Antibody (3.591) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与ADAR3 Antibody (3.591) 搭配使用(请参阅下面的订购信息)。

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ADAR3抗体(3.591)是一种小鼠单克隆IgG抗体,可通过蛋白质印迹(WB)和免疫沉淀(IP)检测小鼠、大鼠和人类来源的ADAR3蛋白。抗ADAR3抗体(3.591)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物,可满足不同实验需求。ADAR3蛋白在RNA编辑中起着至关重要的作用,特别是在双链RNA结构中,将腺嘌呤残基修饰为肌苷,这对调节基因表达和产生蛋白质多样性至关重要。这种编辑过程可能会改变mRNA转录本的编码潜力,最终影响细胞功能和反应。ADAR家族(包括ADAR3)的特征是具有一系列双链RNA结合基序和C端催化结构域,这对酶活性至关重要。了解ADAR3的功能及其与其他RNA分子的相互作用对于阐明其在神经过程中的作用以及其在包括神经疾病和癌症在内的各种疾病中的潜在影响非常重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ADAR3 抗体 (3.591) 参考文献:

  1. AMPA 受体基因的点突变可挽救缺乏 RNA 编辑酶 ADAR2 的小鼠的致死率。  |  Higuchi, M., et al. 2000. Nature. 406: 78-81. PMID: 10894545
  2. ADAR1 和 ADAR2 的底物识别。  |  Wong, SK., et al. 2001. RNA. 7: 846-58. PMID: 11421361
  3. ADAR2 A-->I 编辑:位点选择性和编辑效率是两个独立的事件。  |  Källman, AM., et al. 2003. Nucleic Acids Res. 31: 4874-81. PMID: 12907730
  4. ADAR2的功能性核固着对RNA编辑的调控  |  Sansam, CL., et al. 2003. Proc Natl Acad Sci U S A. 100: 14018-23. PMID: 14612560
  5. ADAR2 底物 RNA 编辑所需的结构和序列决定因素。  |  Dawson, TR., et al. 2004. J Biol Chem. 279: 4941-51. PMID: 14660658
  6. 核仁中 ADAR2 介导的 RNA 底物编辑受到 C/D 小核仁 RNA 的抑制。  |  Vitali, P., et al. 2005. J Cell Biol. 169: 745-53. PMID: 15939761
  7. 肌醇六磷酸与 ADAR2 核心结合,是 RNA 编辑所必需的。  |  Macbeth, MR., et al. 2005. Science. 309: 1534-9. PMID: 16141067
  8. 缺乏 ADAR2 自动调节功能的小鼠体内 RNA 编辑发生了改变。  |  Feng, Y., et al. 2006. Mol Cell Biol. 26: 480-8. PMID: 16382140

订购信息

产品名称产品编号规格价格数量收藏夹

ADAR3 抗体 (3.591)

sc-73410
200 µg/ml
$316.00

ADAR3 (3.591): m-IgG Fc BP-HRP 套装

sc-525698
200 µg Ab; 10 µg BP
$354.00

ADAR3 抗体 (3.591) AC

sc-73410 AC
500 µg/ml, 25% agarose
$416.00

ADAR3 抗体 (3.591) HRP

sc-73410 HRP
200 µg/ml
$316.00

ADAR3 抗体 (3.591) FITC

sc-73410 FITC
200 µg/ml
$330.00

ADAR3 抗体 (3.591) PE

sc-73410 PE
200 µg/ml
$343.00

ADAR3 抗体 (3.591) Alexa Fluor® 488

sc-73410 AF488
200 µg/ml
$357.00

ADAR3 抗体 (3.591) Alexa Fluor® 546

sc-73410 AF546
200 µg/ml
$357.00

ADAR3 抗体 (3.591) Alexa Fluor® 594

sc-73410 AF594
200 µg/ml
$357.00

ADAR3 抗体 (3.591) Alexa Fluor® 647

sc-73410 AF647
200 µg/ml
$357.00

ADAR3 抗体 (3.591) Alexa Fluor® 680

sc-73410 AF680
200 µg/ml
$357.00

ADAR3 抗体 (3.591) Alexa Fluor® 790

sc-73410 AF790
200 µg/ml
$357.00

What strain of HeLa cells were used for the ADAR3 Antibody (3.591): sc-73410 western blots? Were these HeLa-CCL2 or H1-HeLa's or a different strain? Thank you.

Asked by: jmoldova
Thank you for your question . We use normal Hela whole cell lysate.
Answered by: BlakeJ
Date published: 2023-01-19

How much sample do you recommend loading on the gel for Western blot detection using ADAR3 (3.591): sc-73410?

Asked by: DefinitelyNotMatt
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Reliable productsThe ADAR3 we purchased was mouse monoclonal IgG, which is directed against synthetic peptides and is recommended for use in specific applications for the detection of mice by WB and IP
Date published: 2017-02-09
Rated 5 out of 5 by from Excellent!This antibody works great in western blot analysis of ADAR3 expression in HeLa whole cell lysate and FLAG-tagged human recombinant ADAR3.
Date published: 2017-01-17
Rated 4 out of 5 by from Clean band in western blot using OrigeneClean band in western blot using Origene ADAR3 transfected whole cell lysates. -SCBT QC
Date published: 2015-03-28
Rated 4 out of 5 by from Robust bands present in western blot analysisRobust bands present in western blot analysis of ADAR3 expression in HeLa whole cell lysate and FLAG-tagged human recombinant ADAR3. -SCBT QC
Date published: 2013-02-06
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ADAR3 抗体 (3.591) is rated 4.5 out of 5 by 4.
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