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Nicotinic Acetylcholine Receptor alpha 7/CHRNA7 Antibody (319) - Immunohistochemistry - Image 157171
AChRα7 (319): sc-58607. 福尔马林固定, 石蜡包埋的人肾上腺组织的免疫过氧化物酶染色显示腺细胞亚群的胞浆染色.
AChRα7 (319): sc-58607. 福尔马林固定, 石蜡包埋的人肾上腺组织的免疫过氧化物酶染色显示腺细胞亚群的胞浆染色.

Nicotinic Acetylcholine Receptor alpha 7/CHRNA7 抗体 (319): sc-58607

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寫評論提問

说明书
  • Nicotinic Acetylcholine Receptor alpha 7/CHRNA7 抗体 319 是大鼠单克隆 IgG1，Nicotinic Acetylcholine Receptor alpha 7/CHRNA7抗体, 在32篇文献中引用，规格为200 µg/ml
  • 针对细菌表达的chicken来源的AChRα7大细胞质域
  • Nicotinic Acetylcholine Receptor alpha 7/CHRNA7 抗体 (319) 推荐用于 WB, IP, IF, IHC(P) 和 FCM，检测mouse, rat, human 和avian 来源的 both native and denatured nicotinic AChRα7; 不会与 AChRα8产生交联反应
  • 抗Nicotinic Acetylcholine Receptor alpha 7/CHRNA7抗体（319）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Nicotinic Acetylcholine Receptor alpha 7/CHRNA7 (319): sc-58607的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成Nicotinic Acetylcholine Receptor alpha 7/CHRNA7 抗体 (319)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 AChR 抗体 包括 AChR α, AChR α 1, AChR α 2, AChR α 3, AChR α 4, AChR α 5, AChR α 6, AChR α 7, AChR α 8和AChR α 9

AChRα7抗体（319）是一种大鼠单克隆IgG1抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和流式细胞术（FCM）检测小鼠、大鼠、人类和鸟类来源的AChRα7蛋白。AChRα7抗体（319）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。AChRα7，也称为NACHRA7、CHRNA7-2或CHRNA7，是烟碱型乙酰胆碱受体家族的关键组成部分，在快速突触传递中起着至关重要的作用。AChRα7是一种502个氨基酸的多通道膜蛋白，可形成同源五聚体，从而形成离子流动所需的跨膜水孔。AChRα7主要位于神经肌肉连接的突触后膜，结合乙酰胆碱可促进离子流入，从而引起肌肉收缩。AChRα7的正常功能对于神经肌肉信号传递至关重要，失调与多种神经系统疾病有关。AChRα7与RIC-3相互作用，后者是一种烟碱型乙酰胆碱受体相关蛋白，对于受体的正常折叠和运输至关重要，进一步强调了AChRα7在维持突触完整性和功能方面的意义。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Nicotinic Acetylcholine Receptor alpha 7/CHRNA7 抗体 (319) 参考文献:

  1. 利用傅立叶变换红外差分光谱探测尼古丁乙酰胆碱受体的构象变化。  |  Baenziger, JE., et al. 1992. Biophys J. 62: 64-6. PMID: 1600101
  2. 大脑中的配体门控离子通道：氨基酸受体超家族。  |  Betz, H. 1990. Neuron. 5: 383-92. PMID: 1698394
  3. 乙酰胆碱酯酶再激活剂改变了尼古丁乙酰胆碱受体离子通道的功能特性。  |  Alkondon, M., et al. 1988. J Pharmacol Exp Ther. 245: 543-56. PMID: 2452874
  4. 电针通过激活术后肠梗阻中α7nAChR介导的JAK2/STAT3信号通路改善肠道炎症。  |  Yang, NN., et al. 2021. Theranostics. 11: 4078-4089. PMID: 33754049
  5. 减弱α7烟碱乙酰胆碱受体（α7nAChR）可通过oxi-CaMKII/MAPK/AP-1轴介导的线粒体氧化应激来挽救淀粉样β诱导的心房重塑。  |  Zhao, J., et al. 2023. Redox Biol. 59: 102594. PMID: 36603528
  6. 经皮耳迷走神经刺激通过下丘脑α7nAchR/JAK2/STAT3/NF-κB通路对大鼠慢性不可预测轻度应激抑郁样行为产生的抗神经炎症作用。  |  Chen, Y., et al. 2023. CNS Neurosci Ther. 29: 2634-2644. PMID: 37032645
  7. 急性肺损伤中AT2细胞中的α7nAChR激活通过WNT7B信号促进肺泡再生。  |  Chen, X., et al. 2023. JCI Insight. 8: PMID: 37410546
  8. 神经递质的作用：打开配体门控离子通道。  |  Unwin, N. 1993. Cell. 72 Suppl: 31-41. PMID: 7679054
  9. 神经元烟碱乙酰胆碱受体的多样性。  |  Sargent, PB. 1993. Annu Rev Neurosci. 16: 403-43. PMID: 7681637
  10. 神经递质的定量释放和长期电位。  |  Stevens, CF. 1993. Cell. 72 Suppl: 55-63. PMID: 8094037
  11. NMDA 受体在信息处理中的作用。  |  Daw, NW., et al. 1993. Annu Rev Neurosci. 16: 207-22. PMID: 8460891
  12. α5亚基对神经元乙酰胆碱受体通道的功能贡献。  |  Ramirez-Latorre, J., et al. 1996. Nature. 380: 347-51. PMID: 8598930

订购信息

产品名称产品编号规格价格数量收藏夹

Nicotinic Acetylcholine Receptor alpha 7/CHRNA7 抗体 (319)

sc-58607
200 µg/ml
$316.00

Nicotinic Acetylcholine Receptor alpha 7/CHRNA7 抗体 (319) AC

sc-58607 AC
500 µg/ml, 25% agarose
$416.00

Nicotinic Acetylcholine Receptor alpha 7/CHRNA7 抗体 (319) HRP

sc-58607 HRP
200 µg/ml
$316.00

Nicotinic Acetylcholine Receptor alpha 7/CHRNA7 抗体 (319) FITC

sc-58607 FITC
200 µg/ml
$330.00

Nicotinic Acetylcholine Receptor alpha 7/CHRNA7 抗体 (319) PE

sc-58607 PE
200 µg/ml
$343.00

Nicotinic Acetylcholine Receptor alpha 7/CHRNA7 抗体 (319) Alexa Fluor® 488

sc-58607 AF488
200 µg/ml
$357.00

Nicotinic Acetylcholine Receptor alpha 7/CHRNA7 抗体 (319) Alexa Fluor® 546

sc-58607 AF546
200 µg/ml
$357.00

Nicotinic Acetylcholine Receptor alpha 7/CHRNA7 抗体 (319) Alexa Fluor® 594

sc-58607 AF594
200 µg/ml
$357.00

Nicotinic Acetylcholine Receptor alpha 7/CHRNA7 抗体 (319) Alexa Fluor® 647

sc-58607 AF647
200 µg/ml
$357.00

Nicotinic Acetylcholine Receptor alpha 7/CHRNA7 抗体 (319) Alexa Fluor® 680

sc-58607 AF680
200 µg/ml
$357.00

Nicotinic Acetylcholine Receptor alpha 7/CHRNA7 抗体 (319) Alexa Fluor® 790

sc-58607 AF790
200 µg/ml
$357.00

Hi! We have produced mouse cells where the last exons of the AChRα7 gene have been deleted (exons 7 to 10). Still a small portion (N-terminal) of the AChRα7 is synthesized and express on the cells. Will this antibody bind to that protein fragment?

Asked by: Saray
Thank you for your question. AChRα7 Antibody (319): sc-58607 was raised against bacterially expressed large cytoplasmic domain of AChRα7 of chicken origin. The epitope region of the antibody is between amino acids 319-469 of accession number P22770. The exact binding region of this antibody within the antigen has not been mapped. Please contact our Technical Service if you have any further questions or to arrange for a free sample of this antibody.
Answered by: Technical Support Europe
Date published: 2019-02-19

What is the approximate amino acid region that this specific antibody is supposed to bind?

Asked by: Dr Cain
Thank you for your question. This antibody was raised against bacterially expressed large cytoplasmic domain of AChRα7 of chicken origin. We have not characterized the exact epitope of this monoclonal antibody.
Answered by: Tech Service
Date published: 2019-01-07

I would like to perform a double stain with sc-58607. Do you have any suggestions?

Asked by: Cweed
We recommend using a directly conjugated primary antibody. This product, sc-58607, is available conjugated to FITC, AF488, and AF647.
Answered by: Technical Support 7
Date published: 2022-08-08
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Rated 5 out of 5 by from specific bandsNo background or non-specific bands .We only see specific bands at 55kda,loading HUVEC cell lysates 50ug,diluted 1:300.
Date published: 2018-01-27
Rated 5 out of 5 by from recommendWestern blot: robust band seen in WB in rat small intestine tissue
Date published: 2017-09-07
Rated 5 out of 5 by from This worked well in WB.Used for WB with human endothelial cells, it always work well.
Date published: 2017-06-01
Rated 1 out of 5 by from ProblemasGreetings, Yes, I would like to write a review on this new Rat Anti-Alpha 7 Nicotinic Acetylcholine receptor antibody. My experience with this antibody has been negative. I also have a work colleague, which has the discontinued polyclonal Alpha 7 Nicotinic Acetylcholine receptor antibody, and it is better than the new rat monoclonal Santa Cruz offers and promotes. Nevertheless, this does not mean that the polyclonal antibody was great either, she had to do Immunoprecipitation (IP) of the protein (using a total of 500 ug in her cell lysate) in order to obtain high enough concentration of the protein in macrophage and microglial lysates, to observe a band in her Western Blot. Her last IP using the new antibody did not even detect a band. She is using the Pierce ECL system, the Femto chemiluminescence system to detect HRP-conjugated secondaries. Both antibodies were used at a concentration of 1:500 in TBS-T and 5% powdered fat-free Milk, overnigt incubation at 4C. I have tried the new Rat-monoclonal Alpha 7 antibody in whole mouse brain lysate and cerebellum, using enormous quantities of protein (80 and 100ug total protein per well) in a normal Western Blot (not performing IP) and the signal is still extremely faint. I also looked at the protein left in the 'pellet' of the lysate, and the pellet showed also faint almost invisible amounts. The dilution I used was 1:200 in TBS-T and 5% powdered fat-free Milk, overnight incubation at 4C. I use the LI-COR Infrared system of detection, using secondary antibodies that are excited at IR wavelengths. I definitely feel that this is not a practical antibody for Western blotting. Its titer is extremely low and normally one would expect to add a max amount of 20-40ug of a cell lysate to observe proteins. The fact that my colleague did not obtain a signal performing an IP or that I could only detect a faint, non-quantifiable band by densitometry, makes this antibody really useless to us. My recommendation: not god for WB, increase the titer of this antibody at least 5X. Thank you for your attention, -Yancy
Date published: 2017-04-15
Rated 3 out of 5 by from It worksPositive data from intestinal cells by Western and IF.
Date published: 2017-02-24
Rated 5 out of 5 by from Strong and specificCan't go wrong with AChR alpha 7 Antibody (319). Observed nice specific signal by IHCP and WB. No background
Date published: 2017-01-23
Rated 5 out of 5 by from worked greatThis antibody worked great for IP and Western blot in rat small intestine tissue.
Date published: 2017-01-05
Rated 4 out of 5 by from ImmunofluorescenceI used these antibodies for IF in transfected with CHRNA7 cells. Gave a strong signal. The antibody worked in WB too, the major product was around 55KDa, but gave few unspecific smaller bands.
Date published: 2016-12-20
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Nicotinic Acetylcholine Receptor alpha 7/CHRNA7抗体 (319) is rated 4.1 out of 5 by 11.
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