Date published: 2025-9-6

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A-FABP 抗体 (B-4): sc-271529

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说明书
  • A-FABP 抗体 B-4 是小鼠单克隆 IgG1 κ,A-FABP抗体, 在53篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的A-FABP的C-terminus的氨基酸106-132之间
  • A-FABP 抗体 (B-4) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 A-FABP
  • 抗A-FABP抗体(B-4)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取A-FABP (B-4): sc-271529免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是A-FABP Antibody (B-4) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与A-FABP Antibody (B-4) 打包提供(请参阅下面的订购信息)。

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関連項目

A-FABP抗体(B-4)是一种IgG1 κ小鼠单克隆A-FABP抗体(也被命名为FABP4抗体、脂肪细胞脂质结合蛋白抗体或ALBP抗体),它通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的A-FABP蛋白。A-FABP抗体(B-4)既可以作为非共轭的抗A-FABP抗体形式,也可以作为多种共轭形式的抗A-FABP抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。脂肪酸结合蛋白,被命名为FABPs,是一组同源的、细胞质蛋白,它们以高度组织特异性的方式表达,并在脂质和碳水化合物代谢的平衡中发挥不可或缺的作用。FABPs介导脂肪酸(FA)和/或疏水性配体在其各自组织内的摄取、转运和定位。这些作用背后的机制可以引起脂肪酸的被动扩散摄取和蛋白质介导的跨膜转运。FABPs在脂肪细胞(A-FABP)、脑(B-FABP)、表皮(E-FABP,也被命名为银屑病相关FABP或PA-FABP)、肌肉和心脏(H-FABP,也被命名为乳腺来源的生长抑制剂或MDGI)、肠(I-FABP)、肝脏(L-FABP)、髓鞘(M-FABP)和睾丸(T-FABP)中表达。人类A-FABP基因由4个外显子组成,定位于8号染色体q21位置,并编码一个由132个氨基酸组成的蛋白质。A-FABP蛋白约占人脂肪组织总胞质蛋白的1%。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

A-FABP 抗体 (B-4) 参考文献:

  1. 脂肪酸结合蛋白的脂肪酸转运功能。  |  Storch, J. and Thumser, AE. 2000. Biochim Biophys Acta. 1486: 28-44. PMID: 10856711
  2. 揭示细胞脂肪酸结合蛋白的意义。  |  Glatz, JF. and Storch, J. 2001. Curr Opin Lipidol. 12: 267-74. PMID: 11353329
  3. 神经组织的脂肪酸结合蛋白  |  Veerkamp, JH. and Zimmerman, AW. 2001. J Mol Neurosci. 16: 133-42; discussion 151-7. PMID: 11478368
  4. 人类脂肪细胞脂质结合蛋白:蛋白质的纯化及其互补 DNA 的克隆。  |  Baxa, CA., et al. 1989. Biochemistry. 28: 8683-90. PMID: 2481498
  5. A-FABP与肥胖和乳腺癌有关。  |  Greenhill, C. 2018. Nat Rev Endocrinol. 14: 566. PMID: 30143798
  6. 代谢性疾病中的A-FABP及其治疗意义:最新进展。  |  Li, HL., et al. 2021. Int J Mol Sci. 22: PMID: 34502295
  7. 使用机器学习和基于分子对接的虚拟筛选,发现Cobimetinib是一种新型A-FABP抑制剂。  |  Yang, S., et al. 2022. RSC Adv. 12: 13500-13510. PMID: 35520131
  8. 早期诊断为妊娠糖尿病的妇女的脂联素、A-FABP和FGF-19水平。  |  Karasek, D., et al. 2022. J Clin Med. 11: PMID: 35566542
  9. 循环AFABP、FGF21和PEDF水平作为威胁视力的糖尿病视网膜病变的预后生物标志物。  |  Lee, CH., et al. 2023. J Clin Endocrinol Metab. 108: e799-e806. PMID: 36856742
  10. 左氧氟沙星通过直接抑制A-FABP减轻脑缺血和再灌注后的血脑屏障破坏。  |  Yang, S., et al. 2024. Eur J Pharmacol. 963: 176275. PMID: 38113968
  11. 细胞质脂肪酸结合蛋白:它们的结构和基因。  |  Veerkamp, JH. and Maatman, RG. 1995. Prog Lipid Res. 34: 17-52. PMID: 7644552
  12. 通过脂肪细胞脂肪酸结合蛋白 aP2 的靶向突变使肥胖与胰岛素抵抗脱钩。  |  Hotamisligil, GS., et al. 1996. Science. 274: 1377-9. PMID: 8910278

订购信息

产品名称产品编号规格价格数量收藏夹

A-FABP 抗体 (B-4)

sc-271529
200 µg/ml
$316.00

A-FABP (B-4): m-IgG Fc BP-HRP 套装

sc-529203
200 µg Ab; 10 µg BP
$354.00

A-FABP (B-4): m-IgGκ BP-HRP 套装

sc-521970
200 µg Ab, 40 µg BP
$354.00

A-FABP (B-4): m-IgG1 BP-HRP 套装

sc-543383
200 µg Ab; 20 µg BP
$354.00

A-FABP 抗体 (B-4) AC

sc-271529 AC
500 µg/ml, 25% agarose
$416.00

A-FABP 抗体 (B-4) HRP

sc-271529 HRP
200 µg/ml
$316.00

A-FABP 抗体 (B-4) FITC

sc-271529 FITC
200 µg/ml
$330.00

A-FABP 抗体 (B-4) PE

sc-271529 PE
200 µg/ml
$343.00

A-FABP 抗体 (B-4) Alexa Fluor® 488

sc-271529 AF488
200 µg/ml
$357.00

A-FABP 抗体 (B-4) Alexa Fluor® 546

sc-271529 AF546
200 µg/ml
$357.00

A-FABP 抗体 (B-4) Alexa Fluor® 594

sc-271529 AF594
200 µg/ml
$357.00

A-FABP 抗体 (B-4) Alexa Fluor® 647

sc-271529 AF647
200 µg/ml
$357.00

A-FABP 抗体 (B-4) Alexa Fluor® 680

sc-271529 AF680
200 µg/ml
$357.00

A-FABP 抗体 (B-4) Alexa Fluor® 790

sc-271529 AF790
200 µg/ml
$357.00

A-FABP (B-4) 中和勝肽

sc-271529 P
100 µg/0.5 ml
$68.00

what should be used to dilute your antibody? if I want to do western blot,how much antibody should be add to EVs sample?

Asked by: Shirley123
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-02-09

Hello, can I use the HRP-conjugated form of sc-271529 to perform not only WB but IP experiments as well? Thank you!

Asked by: ck1916
Thank you for your question. The HRP-conjugate of this antibody is only recommended for detection of A-FABP in western blots, IHC(P), and ELISA. For IP experiments we recommend using the unconjugated version of the antibody, or ideally the agarose conjugate. Please feel free to reach out to our technical service directly if you have additional questions.
Answered by: Tech Support Europe
Date published: 2019-09-17

What is the difference between sc-271529 and sc-271529 P?

Asked by: jenni
Thank you for your question. sc-271529 P is our blocking peptide, meant as a negative control for use with the A-FABP (B-4): sc-271529 monoclonal antibody.
Answered by: Technical Support 4
Date published: 2017-01-13

What secondary is best to use with A-FABP (B-4): sc-271529?

Asked by: jenni
Thank you for your question. While most anti-mouse IgG secondary antibodies are appropriate for use with A-FABP (B-4):sc-271529, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 4
Date published: 2017-01-13

I would like to know A-FABP Antibody (B-4) sc-271529 200 µg/ml, how much volume in a pack.

Asked by: Sreelekha
Thank you for your question. You receive 200µg of antibody in 1 ml. If you have any further questions, please contact our Asia Technical Service team. You can reach them by phone at (86 21) 6093-6350, by email at: asia@scbio.cn or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2016-11-18
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Rated 3 out of 5 by from Working antibodyMultiple bands were detected but still expected size band was detected
Date published: 2021-12-03
Rated 5 out of 5 by from Very good antibody for WB detectionVery nice western blot data (murine adipose tissue samples) Strong signal with low background
Date published: 2021-03-26
Rated 5 out of 5 by from GREATtestes in human adipose tissue 3ug overnight. Specific.
Date published: 2018-05-11
Rated 5 out of 5 by from works great for WBI tested with 3T3L1 cells lysate by western blotting. It detected pretty strong specific band.
Date published: 2018-05-02
Rated 5 out of 5 by from Nice, Clean BandsI received this antibody as a sample to determine differentiation in 3T3-L1 adipocytes. The western blot provided clean bands without background. I am pleased
Date published: 2018-02-23
Rated 5 out of 5 by from Great featuresI bought this 3weeks ago and am so happy that I did Western Blot (dilution 1:100)
Date published: 2017-04-14
Rated 5 out of 5 by from Outstanding western dataVery nice western blot data; strong signal with low background
Date published: 2017-01-23
Rated 5 out of 5 by from Great for WBThis A-FABP Antibody shows great results in WB for mouse and rat samples.
Date published: 2017-01-14
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A-FABP 抗体 (B-4) is rated 4.9 out of 5 by 14.
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