Date published: 2025-9-5
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5-LO Antibody (33) - Western Blotting - Image 399008
5-LO 抗体 (33): sc-136195. A-431 (A), NAMALWA (B) 和 RT-4 (C) 全细胞裂解液及人脾组织提取物 (D) 中 5-LO 表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.
5-LO 抗体 (33): sc-136195. A-431 (A), NAMALWA (B) 和 RT-4 (C) 全细胞裂解液及人脾组织提取物 (D) 中 5-LO 表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.

5-LO 抗体 (33): sc-136195

4.5(8)
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说明书
  • 5-LO抗体(33)是小鼠单克隆IgG1 κ 5-LO抗体, 在18篇文献中引用，规格为200 µg/ml
  • 针对human起源的5-LO的C-terminus部分
  • 推荐用于 mouse, rat， human 和 Arabadopsis 来源的5-LO WB, IP 和 IF检测; 不建议用于IP
  • 关于如何获取5-LO (33): sc-136195的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是5-LO Antibody (33) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与5-LO Antibody (33) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

附加LO抗体，包括12-LO, 15-LO, 15-LO2和5-LO

5-LO抗体（33）是一种IgG1 κ小鼠单克隆5-LO抗体（也被称为LOX5抗体、花生四烯酸5-脂氧合酶抗体、多不饱和脂肪酸5-脂氧合酶抗体、5-LOX抗体、LOG5抗体、花生四烯酸5-脂氧合酶抗体、白三烯A4合成酶抗体、5-脂氧合酶抗体、LOX-5抗体或5LPG抗体），它可以通过WB、IP和IF检测到小鼠、大鼠、人类和其他来源的5-LO蛋白。5-LO抗体（33）以非偶联的抗5-LO抗体形式提供。5-脂氧合酶（5-LO）主要在多形核白细胞、巨噬细胞和肥大细胞中表达。5-LO在白细胞三烯的生物合成中执行前两个催化反应。白细胞三烯是一种脂质代谢物，可在过敏反应期间诱导气道平滑肌收缩和增加血管通透性。5-LO的细胞定位因细胞类型而异。在活化的血液多形核白细胞中，5-LO经历钙依赖性易位，从细胞质转移到核膜。在肺泡巨噬细胞中，大多数5-LO位于细胞核中，并且在这些细胞被激活时，核内5-LO与核膜结合。5-LO的这种细胞内穿梭依赖于与各种信号分子的结合、磷酸化以及独特的核定位信号的存在，该信号在5-LO的氨基末端编码。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

5-LO 抗体 (33) 参考文献:

  1. 鉴定 5-脂氧合酶核导入所需的双核定位序列。  |  Healy, AM., et al. 1999. J Biol Chem. 274: 29812-8. PMID: 10514459
  2. 5-脂氧合酶mRNA和蛋白质同工型。  |  Ochs, MJ., et al. 2014. Basic Clin Pharmacol Toxicol. 114: 78-82. PMID: 24020397
  3. 人 5-脂氧合酶的分子克隆和氨基酸序列。  |  Matsumoto, T., et al. 1988. Proc Natl Acad Sci U S A. 85: 26-30. PMID: 2829172
  4. 抑制5-脂氧合酶（5-Lo）可减轻脂多糖（Lps）诱导的牙周炎中的炎症和骨吸收。  |  Lopes, DEM., et al. 2017. J Periodontol. 1-18. PMID: 28871891
  5. 5-脂氧合酶对微RNA加工的调节。  |  Uebbing, S., et al. 2021. FASEB J. 35: e21193. PMID: 33205517
  6. 5-脂氧合酶作为抗衰老相关脑部疾病的新兴靶点。  |  Yan, M., et al. 2021. Ageing Res Rev. 69: 101359. PMID: 33984528
  7. 在关节挛缩模型中，使用5-脂氧合酶（5-LO）抑制剂抑制白三烯的作用。  |  Jeffs, AD., et al. 2023. J Exp Orthop. 10: 64. PMID: 37341811
  8. 在静止的人肺泡巨噬细胞中，5-脂氧合酶位于细胞核的外染色质中，并在细胞活化时转移到核膜上。  |  Woods, JW., et al. 1995. J Clin Invest. 95: 2035-46. PMID: 7738170
  9. 花生四烯酸对人类中性粒细胞磷脂酶 A2 活性指数的影响  |  Winkler, JD., et al. 1993. Biochem J. 291 (Pt 3): 825-31. PMID: 8387780
  10. 酪氨酸激酶活性调节细胞 5-脂氧合酶的催化和转运。  |  Lepley, RA., et al. 1996. J Biol Chem. 271: 6179-84. PMID: 8626407
  11. 细胞膜磷脂酶 A2, 5-脂氧合酶和 5-脂氧合酶激活蛋白在 A23187 刺激的人中性粒细胞核膜上的共定位。  |  Pouliot, M., et al. 1996. Eur J Biochem. 238: 250-8. PMID: 8665944
  12. 利用绿色荧光蛋白/5-脂氧合酶融合蛋白确定 5-脂氧合酶核定位的因素。  |  Chen, XS., et al. 1998. J Biol Chem. 273: 31237-44. PMID: 9813031

订购信息

产品名称产品编号规格价格数量收藏夹

5-LO 抗体 (33)

sc-136195
200 µg/ml
$316.00

5-LO (33): m-IgG Fc BP-HRP 套装

sc-537376
200 µg Ab; 10 µg BP
$354.00

5-LO (33): m-IgGκ BP-HRP 套装

sc-534626
200 µg Ab; 40 µg BP
$354.00

5-LO (33): m-IgG1 BP-HRP 套装

sc-545162
200 µg Ab; 20 µg BP
$354.00

Would you please link reference/s of IF- ICQ data with this antibody? Preferentially on human cells. Thank you!

Asked by: Sofía
Thank you for contacting Santa Cruz Biotechnology. For assistance please contact technical service. please email scbt@scbt.com or call 800-457-3801.
Answered by: BlakeJ
Date published: 2025-01-16

买5-LO 抗体 (33): sc-136195一抗做wb，可以搭配哪个HRP偶联的二抗，麻烦推荐一个？

Asked by: chenyuan0308
感谢您的提问。请联系我们的技术支持部门。亚洲技术支持电话(+86) 021-60936350、邮箱asia@scbio.cn、或选择网页上Live Chat中的“中文支持”。 Thank you for your inquiry. Please contact our Technical Service Department. Our Asian Technical Service team is available at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Technical Support 8
Date published: 2018-12-21

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 15
Date published: 2017-02-07
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Rated 4 out of 5 by from Good bandsIt works well in western blot studies in liver lysates from rat and mouse samples. It works better in mouse
Date published: 2021-01-18
Rated 5 out of 5 by from Buena señal.Detectó la proteina. Buena señal. Dilucion1/500. Pancreas.
Date published: 2020-09-22
Rated 5 out of 5 by from clean bands on westernAfter testing multiple antibodies for 5-LO in western this gave us the cleanest and most specific results.
Date published: 2017-05-25
Rated 5 out of 5 by from good resultWe used it in Hela cells 1:1000 dilution, which could help us get a clear band.
Date published: 2017-05-13
Rated 5 out of 5 by from Good choice for IF and WB5-LO Antibody (33) is a good choice for IF and WB research applications.
Date published: 2017-01-21
Rated 4 out of 5 by from perfomed great in WB using for human MKN45perfomed great in WB using for human MKN45 total cell lysate
Date published: 2016-12-12
Rated 4 out of 5 by from Good immunofluorescence cytoplasmic and membraneGood immunofluorescence cytoplasmic and membrane staining in A-431 cells. -SCBT QC
Date published: 2015-02-14
Rated 4 out of 5 by from Nice band detected in WB using SLNice band detected in WB using SL-29 whole cell lysate. -SCBT QC
Date published: 2015-01-24
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5-LO 抗体 (33) is rated 4.5 out of 5 by 8.
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