Date published: 2025-9-8
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11β-HSD2 Antibody (C-9) - Western Blotting - Image 362885
11β-HSD2 抗体 (C-9): sc-365529. Caco-2 (A) 和 SK-BR-3 (B) 全细胞裂解液中 11β-HSD2 表达的 Western 印迹分析.
11β-HSD2 抗体 (C-9): sc-365529. Caco-2 (A) 和 SK-BR-3 (B) 全细胞裂解液中 11β-HSD2 表达的 Western 印迹分析.

11β-HSD2 抗体 (C-9): sc-365529

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说明书
  • 11 beta-HSD2 抗体 C-9 是小鼠单克隆 IgG1 κ，11β-HSD2抗体, 在19篇文献中引用，规格为200 µg/ml
  • 免疫human物种的11β-HSD2的氨基酸261-405
  • 11 beta-HSD2 抗体 (C-9) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 11β-HSD2
  • 抗11 beta-HSD2抗体（C-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取11β-HSD2 (C-9): sc-365529的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是11β-HSD2 Antibody (C-9) for WB and IHC(P) applications. 的首选辅助检测试剂，这些试剂现与11β-HSD2 Antibody (C-9) 打包提供（请参阅下面的订购信息）。

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说明书与实验方案
研究信息

関連項目

此外 11 β-HSD 抗体包括 和

11β-HSD2抗体（C-9）是一种IgG1 κ小鼠单克隆11β-HSD2抗体（也被称为DHI2抗体、羟基类固醇11-β脱氢酶2抗体、SDR9C3抗体、短链脱氢酶/还原酶家族9C成员3抗体、11-β-羟基类固醇脱氢酶2型抗体、11-β-HSD 2型抗体、11-HSD 2型抗体、11-β-HSD2抗体、HSD11K抗体、11-DH2抗体、11-β-HSD抗体、AME1抗体或HSD2抗体），它能够通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学（IHC(P)）和酶联免疫吸附测定（ELISA）检测到人源的11β-HSD2蛋白。11β-HSD2抗体（C-9）既有非偶联形式的抗11β-HSD2抗体，也有多种偶联形式的抗11β-HSD2抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。糖皮质激素在靶组织中的作用受到11β-羟基类固醇脱氢酶（11β-HSD）的调节，该酶能够催化具有激素活性的C11-羟基化皮质类固醇（皮质醇、皮质酮）与其无活性的C11-酮代谢物（可的松、11-脱氢皮质酮）之间的相互转化。11β-HSD至少存在两种异构体：一种是低亲和力的NADP依赖性脱氢酶/氧化还原酶（11β-HSD1），另一种是高亲和力的NAD依赖性脱氢酶（11β-HSD2）。糖基化的11β-HSD1蛋白能够激活可的松转化为皮质醇，该蛋白在哺乳动物中广泛表达，且在肝脏中的表达量最高。11β-HSD2则使皮质醇失活转化为可的松，并在胎盘、醛固酮靶组织（肾脏、腮腺、结肠和皮肤）以及胰腺中表达。11β-HSD1可能在葡萄糖稳态和包括胰岛素抵抗和肥胖在内的多种疾病的发病机制中发挥作用。11β-HSD2与人类结肠上皮的分化或成熟相关，并可能作为发育和疾病的标志物。此外，11β-HSD2在调节糖皮质激素对盐皮质激素和糖皮质激素受体的占用方面发挥着至关重要的作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

11β-HSD2 抗体 (C-9) 参考文献:

  1. 11 β-羟类固醇脱氢酶。  |  Stewart, PM. and Krozowski, ZS. 1999. Vitam Horm. 57: 249-324. PMID: 10232052
  2. 妊娠早期 11 β-羟基类固醇脱氢酶的表达：对人类滋养细胞-子宫内膜相互作用的影响。  |  Arcuri, F., et al. 1999. Semin Reprod Endocrinol. 17: 53-61. PMID: 10406076
  3. 人 11-beta - 羟类固醇脱氢酶 1 型催化结构域的功能表达, 表征和纯化。  |  Walker, EA., et al. 2001. J Biol Chem. 276: 21343-50. PMID: 11294832
  4. 11 β-羟基类固醇脱氢酶同工酶在人眼中的表达和假定作用。  |  Rauz, S., et al. 2001. Invest Ophthalmol Vis Sci. 42: 2037-42. PMID: 11481269
  5. 11beta-羟基类固醇脱氢酶 1 型无效小鼠的血脂和脂蛋白概况, 肝脏胰岛素敏感性和葡萄糖耐受性得到改善。  |  Morton, NM., et al. 2001. J Biol Chem. 276: 41293-300. PMID: 11546766
  6. 人类 11 β-羟基类固醇脱氢酶基因。结构, 组织分布和染色体定位。  |  Tannin, GM., et al. 1991. J Biol Chem. 266: 16653-8. PMID: 1885595
  7. 胎儿和胎盘 11β-HSD2：发育编程的枢纽。  |  Cottrell, EC., et al. 2014. Acta Physiol (Oxf). 210: 288-95. PMID: 24330050
  8. 11β-Hydroxysteroid dehydrogenase type 2 (11β-HSD2)抑制剂研究的最新进展。  |  Zhou, C., et al. 2017. Environ Toxicol Pharmacol. 52: 47-53. PMID: 28366868
  9. 胎盘 11β-HSD2 对子痫前期发病机制的贡献。  |  Wang, G., et al. 2020. FASEB J. 34: 15379-15399. PMID: 32978833
  10. 人类 11 β-羟基类固醇脱氢酶 2 型酶的克隆和组织分布。  |  Albiston, AL., et al. 1994. Mol Cell Endocrinol. 105: R11-7. PMID: 7859916
  11. 人胎盘 11 β-羟类固醇脱氢酶 2 型抗血清的克隆与制备。  |  Brown, RW., et al. 1996. Biochem J. 313 (Pt 3): 1007-17. PMID: 8611140
  12. 人类结肠中的 11 β-羟基类固醇脱氢酶 II 型：胎儿发育和肿瘤分化的新标志物。  |  Takahashi, K., et al. 1998. Anticancer Res. 18: 3381-8. PMID: 9858912

订购信息

产品名称产品编号规格价格数量收藏夹

11β-HSD2 抗体 (C-9)

sc-365529
200 µg/ml
$316.00

11β-HSD2 (C-9): m-IgG Fc BP-HRP 套装

sc-527377
200 µg Ab; 10 µg BP
$354.00

11β-HSD2 (C-9): m-IgG1 BP-HRP 套装

sc-532750
200 µg Ab; 20 µg BP
$354.00

11β-HSD2 抗体 (C-9) AC

sc-365529 AC
500 µg/ml, 25% agarose
$416.00

11β-HSD2 抗体 (C-9) HRP

sc-365529 HRP
200 µg/ml
$316.00

11β-HSD2 抗体 (C-9) FITC

sc-365529 FITC
200 µg/ml
$330.00

11β-HSD2 抗体 (C-9) PE

sc-365529 PE
200 µg/ml
$343.00

11β-HSD2 抗体 (C-9) Alexa Fluor® 488

sc-365529 AF488
200 µg/ml
$357.00

11β-HSD2 抗体 (C-9) Alexa Fluor® 546

sc-365529 AF546
200 µg/ml
$357.00

11β-HSD2 抗体 (C-9) Alexa Fluor® 594

sc-365529 AF594
200 µg/ml
$357.00

11β-HSD2 抗体 (C-9) Alexa Fluor® 647

sc-365529 AF647
200 µg/ml
$357.00

11β-HSD2 抗体 (C-9) Alexa Fluor® 680

sc-365529 AF680
200 µg/ml
$357.00

11β-HSD2 抗体 (C-9) Alexa Fluor® 790

sc-365529 AF790
200 µg/ml
$357.00

Is this antibody tested for western blot in mouse samples?

Asked by: Rodrigo
Thank you for your question. Based on our tests with the product, 11β-HSD2 Antibody (C-9): sc-365529 is only recommended for detection of 11β-HSD2 of human origin by WB, IP, IF, IHC(P) and ELISA. We do not recommend it for use on murine cell or tissue samples.
Answered by: Tech Support Europe
Date published: 2025-06-06

Is it possible to detect rat 11-beta-hydroxysteroid dehydrogenase type 2 with a C-9 antibody?

Asked by: HS Kim
Thank you for your question. We have not yet confirmed reactivity with mouse and rat species for this mouse monoclonal antibody, 11β-HSD2 (C-9): sc-365529. The antibody is raised against a recombinant protein sequence that does share 78% sequence identity with the rat 11β-HSD2 protein, so there is a good chance that the antibody will react with rat. We are also aware of a recent 2016 publication citing use of the (C-9) clone in rat heart samples, please see PMID: 27614224. For a limited time, we are offering a free 10 µg sample of this antibody. Please contact our Technical Service Department and we would be happy to send you a sample to test. Please call our Asian Support office toll-free at 800-820-8626, email asia@scbio.cn, or contact us by live chat.
Answered by: Technical Support
Date published: 2017-05-10

What is the recommended fixation method for immunofluorescence staining with 11β-HSD2 (C-9): sc-365529 monoclonal antibody?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Works in rat brain IHCIHC of rat brain (Showing the paraventricular nucleus) with this antibody. Used a 1:1000 dilution.
Date published: 2024-01-13
Rated 5 out of 5 by from Excellent antibodyThis antibody is great for IHC, IF and western blotting.
Date published: 2017-10-20
Rated 5 out of 5 by from Great antibodyI had this antibody as a free sample. I used it to perform an immunostaining using rat brain-stem coronal sections. it works great!
Date published: 2017-08-30
Rated 5 out of 5 by from Nice results in WBWe have nice in-house Western blot results using this 11 beta-HSD2 Antibody (C-9) in Caco-2, JAR and SK-BR-3 whole cell lysates.
Date published: 2017-02-07
Rated 5 out of 5 by from Good specificity in WB in human transfectedGood specificity in WB in human transfected 293T whole cell lysates and human kidney tissue extract. -SCBT QC
Date published: 2015-07-14
Rated 5 out of 5 by from Robust signal in WB in human kidney tissue extractRobust signal in WB in human kidney tissue extract. -SCBT QC
Date published: 2015-07-10
Rated 5 out of 5 by from Strong signal in WB in JAR whole cell lysateStrong signal in WB in JAR whole cell lysate. -SCBT QC
Date published: 2015-05-11
Rated 5 out of 5 by from Produced excellent immunofluorescence cytoplasmicProduced excellent immunofluorescence cytoplasmic and nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-01-11
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11β-HSD2 抗体 (C-9) is rated 5.0 out of 5 by 8.
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