Vimentin Antibody (V9) recommended for detection of Vimentin of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM

Vimentin Antibody (V9): sc-6260

Vimentin Antibody (V9) is rated 4.7 out of 5 by 58.
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Datasheets
    • Vimentin Antibody (V9) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against purified Vimentin from porcine eye lens
    • recommended for detection of Vimentin of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM; also reactive with additional species, including porcine
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to TRITC, PerCP, PerCP-Cy5.5 or Alexa Fluor® 405 for IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact us to receive a FREE 10 µg sample of Vimentin (V9): sc-6260.
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Ordering Information

PRODUCT NAME CATALOG # UNIT PRICE QTY FAVORITES
Vimentin Antibody (V9) sc-6260 200 µg/ml $279.00
Vimentin Antibody (V9) AC sc-6260 AC 500 µg/ml, 25% agarose $366.00
Vimentin Antibody (V9) HRP sc-6260 HRP 200 µg/ml $279.00
Vimentin Antibody (V9) FITC sc-6260 FITC 200 µg/ml $292.00
Vimentin Antibody (V9) PE sc-6260 PE 200 µg/ml $303.00
Vimentin Antibody (V9) Alexa Fluor® 488 sc-6260 AF488 200 µg/ml $314.00
Vimentin Antibody (V9) Alexa Fluor® 546 sc-6260 AF546 200 µg/ml $314.00
Vimentin Antibody (V9) Alexa Fluor® 594 sc-6260 AF594 200 µg/ml $314.00
Vimentin Antibody (V9) Alexa Fluor® 647 sc-6260 AF647 200 µg/ml $314.00
Vimentin Antibody (V9) Alexa Fluor® 680 sc-6260 AF680 200 µg/ml $314.00
Vimentin Antibody (V9) Alexa Fluor® 790 sc-6260 AF790 200 µg/ml $314.00
Vimentin Antibody (V9) TRITC sc-6260 TRITC 200 µg/ml $282.00
Vimentin Antibody (V9) Alexa Fluor® 405 sc-6260 AF405 100 µg/2 ml $314.00
Vimentin Antibody (V9) PerCP sc-6260 PerCP 100 tests in 2 ml $310.00
Vimentin Antibody (V9) PerCP-Cy5.5 sc-6260 PCPC5 100 tests in 2 ml $310.00

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Vimentin Antibody (V9)Product Citations

See how others have used Vimentin Antibody (V9). Click on the entry to view the PubMed entry .

Citations 1 to 10 of 397 total

PMID: # 30092248  Ascenção, CFR. et al. 2018. Biochimie. 154: 69-76.

PMID: # 30082484  Hong, D. et al. 2018. Mol. Cancer Res.

PMID: # 30078003  Zhu, G. et al. 2018. Cell. Physiol. Biochem. 48: 1735-1746.

PMID: # 30240148  Stevenson, ME. et al. 2018. Brain Behav. e01110.

PMID: # 29290468  Margalef, P. et al. 2018. Cell. 172: 439-453.e14.

PMID: # 29463855  Zheng, W. et al. 2018. Sci Rep. 8: 3320.

PMID: # 29202474  Mishra, P. et al. 2018. J. Clin. Invest. 128: 323-340.

PMID: # 29174846  Zeng, HL. et al. 2018. Biochim. Biophys. Acta. 1866: 307-315.

PMID: # 29408667  Zhang, X. et al. 2018. Toxicol In Vitro. 48: 195-204.

PMID: # 29329079  Rider, MA. et al. 2018. Virology. 516: 55-70.

Citations 1 to 10 of 397 total

I am researcher at Trinity College Dublin, Ireland. My work is mainly focused on CTCs, and planning to detect Vimentin marker. Could I get your free sample of FITC- labelled anti-vimentin Ab (Santa-Cruz, ’vimentin cohort’)? Thanks very much. Yanmei

Asked by: Yanmei
Thank you for your question. Unfortunately, we don't offer samples of conjugated antibodies. Please contact our Technical Service Department to request a free 10 µg sample of our mouse monoclonal antibody, AOS-1 (H-9): sc-398080. Please call our European Support office toll-free at 00800 4573 8000 or email to europe@scbt.com
Answered by: Technical Support Europe
Date published: 2017-07-21

Can you confirm if the concentration stays the same from lot to lot?

Asked by: CRUK SH
Thank you for your question. The concentration of IgG provided in each lot is the same. We provide this Vimentin (V9) mouse monoclonal IgG1 at a concentration of 200 µg in 1 ml.
Answered by: Technical Support
Date published: 2017-03-09

I need to use an antibody to detect the protein in samples of porcine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Tech Service 3
Date published: 2016-12-15

How can this mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: jerojero
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal antibody Vimentin Antibody (V9): sc-6260is currently available conjugated to agarose (sc-6260 AC), 500 µg/0.25 ml agarose in 1 ml, for IP; HRP (sc-6260 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-6260 PE), fluorescein (sc-6260 FITC), Alexa Fluor® 488 (sc-6260 AF488) or Alexa Fluor® 647 (sc-6260 AF647), 200 µg/ml, for IF, IHC(P) and FCM.
Answered by: Tech Service 3
Date published: 2016-12-15

Hello! I stained some floating sections, but the slides are not stained at all with DAB incubation for over 10 min. The slides were incubated with Vimentin HRP (1:100) over night and then directly incubated in DAB solution. Can you give me some suggestion

Asked by: Song2016
Thank you for your question. We would be glad to help you with your staining concerns. Please contact our European Technical Service team, and they will be glad to help you! You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: europe@scbt.com or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2016-10-27

Is it possible to order a sample of unconjugated Vimentin Antibody (V9) before purchasing?

Asked by: gradstudent21
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Rated 4 out of 5 by from Great antibodies I got a free sample from company. I used antibodies in WB in dilution 1:1000. It works good.
Date published: 2018-07-14
Rated 5 out of 5 by from Vimentin-Alexa Fluor® 647 I bought Vimentin Antibody (V9) Alexa Fluor® 647 for cardiac fibroblasts immunostaining. Results is good. Fixing procedure: 10 min 4% PFA + Methanol treatment at -20 for 5 min.
Date published: 2018-05-30
Rated 5 out of 5 by from Great product!! It's a great product for me to analyze Western blotting.
Date published: 2018-04-22
Rated 5 out of 5 by from UPENN Gα 12 Antibody is very good. The band is very stronger.
Date published: 2018-04-19
Rated 5 out of 5 by from Très bonne fonctionnalités en immuno histologie Utilisation pour le marquage vimentine de cellules msc humaine en iimmunohistochimie : très bien.
Date published: 2018-04-10
Rated 4 out of 5 by from Works with primary myoblasts I used 200 dilutions for WB on my primary myoblasts isolated from mouse and it worked well. Dilutions were less effective after 1 week, but I don't have enough antibody stock to make fresh dilutions. Will get more and try!
Date published: 2018-03-22
Rated 1 out of 5 by from 特異的なバンドが出にくい Ishikawa細胞 タンパク10 μgを泳動した。非特異的なバンドが多く、特異的なバンドが検出しいにくい。
Date published: 2018-03-16
Rated 5 out of 5 by from Perfect Vimentin Antibody I have used the Vimentin antibody In vivo. I got awesome results of immunofluorescent stainings of both embryonic and adult animals. I used a concentration of 1:100, and I think It can be diluted more (may be up to 1:400). I would recommend this antibody for In vivo detection of mesenchymal (mesodermal) tissue.
Date published: 2017-12-28
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