Vimentin Antibody (V9) recommended for detection of Vimentin of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM

Vimentin Antibody (V9): sc-6260

Vimentin Antibody (V9) is rated 4.6 out of 5 by 61.
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Datasheets
    • Vimentin Antibody (V9) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against purified Vimentin from porcine eye lens
    • recommended for detection of Vimentin of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM; also reactive with additional species, including porcine
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to TRITC, PerCP, PerCP-Cy5.5 or Alexa Fluor® 405 for IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact us to receive a FREE 10 µg sample of Vimentin (V9): sc-6260.
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Is it possible to order a sample of unconjugated Vimentin Antibody (V9) before purchasing?

Asked by: gradstudent21
Thank you for your question. Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody.
Answered by: Technical Support
Date published: 2018-12-05

I am researcher at Trinity College Dublin, Ireland. My work is mainly focused on CTCs, and planning to detect Vimentin marker. Could I get your free sample of FITC- labelled anti-vimentin Ab (Santa-Cruz, ’vimentin cohort’)? Thanks very much. Yanmei

Asked by: Yanmei
Thank you for your question. Unfortunately, we don't offer samples of conjugated antibodies. Please contact our Technical Service Department to request a free 10 µg sample of our mouse monoclonal antibody, AOS-1 (H-9): sc-398080. Please call our European Support office toll-free at 00800 4573 8000 or email to europe@scbt.com
Answered by: Technical Support Europe
Date published: 2017-07-21

Can you confirm if the concentration stays the same from lot to lot?

Asked by: CRUK SH
Thank you for your question. The concentration of IgG provided in each lot is the same. We provide this Vimentin (V9) mouse monoclonal IgG1 at a concentration of 200 µg in 1 ml.
Answered by: Technical Support
Date published: 2017-03-09

I need to use an antibody to detect the protein in samples of porcine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Tech Service 3
Date published: 2016-12-15

How can this mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: jerojero
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal antibody Vimentin Antibody (V9): sc-6260is currently available conjugated to agarose (sc-6260 AC), 500 µg/0.25 ml agarose in 1 ml, for IP; HRP (sc-6260 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-6260 PE), fluorescein (sc-6260 FITC), Alexa Fluor® 488 (sc-6260 AF488) or Alexa Fluor® 647 (sc-6260 AF647), 200 µg/ml, for IF, IHC(P) and FCM.
Answered by: Tech Service 3
Date published: 2016-12-15

Hello! I stained some floating sections, but the slides are not stained at all with DAB incubation for over 10 min. The slides were incubated with Vimentin HRP (1:100) over night and then directly incubated in DAB solution. Can you give me some suggestion

Asked by: Song2016
Thank you for your question. We would be glad to help you with your staining concerns. Please contact our European Technical Service team, and they will be glad to help you! You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: europe@scbt.com or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2016-10-27
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Rated 3 out of 5 by from Tested on HFFF by FACS This antibody was tested on HFFF cells by FACS and gave a mild signal, even at dilution 1:25
Date published: 2018-11-19
Rated 5 out of 5 by from Excellent antibody I got a sample of this antibody and it is very good.
Date published: 2018-11-19
Rated 3 out of 5 by from WB Clear band in HeLa cells but no band in KM-12 in WB. Tested concentrations; 1:500 and 1:1000 with 50 ug of cell lysate. Blokcing and antibodies in 5%Milk/TBST
Date published: 2018-11-06
Rated 4 out of 5 by from Great antibodies I got a free sample from company. I used antibodies in WB in dilution 1:1000. It works good.
Date published: 2018-07-14
Rated 5 out of 5 by from Vimentin-Alexa Fluor® 647 I bought Vimentin Antibody (V9) Alexa Fluor® 647 for cardiac fibroblasts immunostaining. Results is good. Fixing procedure: 10 min 4% PFA + Methanol treatment at -20 for 5 min.
Date published: 2018-05-30
Rated 5 out of 5 by from Great product!! It's a great product for me to analyze Western blotting.
Date published: 2018-04-22
Rated 5 out of 5 by from UPENN Gα 12 Antibody is very good. The band is very stronger.
Date published: 2018-04-19
Rated 5 out of 5 by from Très bonne fonctionnalités en immuno histologie Utilisation pour le marquage vimentine de cellules msc humaine en iimmunohistochimie : très bien.
Date published: 2018-04-10
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