Integrin alpha M Antibody (2LPM19c) recommended for detection of Integrin αM of mouse, rat and human origin by IF and FCM

Integrin αM Antibody (2LPM19c): sc-20050

Integrin αM Antibody (2LPM19c) is rated 4.4 out of 5 by 7.
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Datasheets
    • Integrin αM Antibody (2LPM19c) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against purified C3bi receptor
    • recommended for detection of Integrin αM of mouse, rat and human origin by IF and FCM
    • available conjugated to agarose for IP; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact us to receive a FREE 10 µg sample of Integrin αM (2LPM19c): sc-20050.
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What negative control should be used when using the conjugated version of Integrin αM (2LPM19c): sc-20050 monoclonal antibody in flow cytometry?

Asked by: jenni
Thank you for your inquiry. We recommend using the isotype specific normal mouse IgG conjugated to the same fluorophore. For example, if you are using sc-20050 PE, then you would want to use normal mouse IgG1 PE, sc-2866.
Answered by: Technical Support 13
Date published: 2017-01-20

How can Integrin αM (2LPM19c): sc-20050 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: jenni
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. Integrin αM (2LPM19c): sc-20050 monoclonal antibody is currently available conjugated to phycoerythrin (sc-20050 PE), fluorescein (sc-20050 FITC), Alexa Fluor® 488 (sc-20050 AF488) or Alexa Fluor® 647 (sc-20050 AF647)
Answered by: Technical Support 2
Date published: 2017-01-16
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Rated 5 out of 5 by from not good for IHC-P I have not observed expected signal in placenta by IHC-P
Date published: 2017-04-06
Rated 5 out of 5 by from Superb antibody Strong western data; also great product for IHC and IF staining
Date published: 2017-01-25
Rated 5 out of 5 by from Good choice! This antibody will help you get your desired results. It has great versatility in mouse, rat, and human samples and will perform great in you WB or IF. It has been used by several researchers to publish.
Date published: 2017-01-17
Rated 1 out of 5 by from does not work on macaque tissue Antibody did not detect Integrin alpha M in paraffin embedded macaque retina tissue when used at a dilution of 1:50 ON
Date published: 2016-12-20
Rated 5 out of 5 by from Great antibody for flow cytometry Great antibody for flow cytometry, good data with human peripheral blood leukocytes. -SCBT QC
Date published: 2015-03-27
Rated 5 out of 5 by from Produced good quality flow cytometry data Produced good quality flow cytometry data -SCBT QC
Date published: 2015-01-23
Rated 5 out of 5 by from Clean blot with single strong band in human Clean blot with single strong band in human PBL whole cell lysate immunoprecipitated with Integrin M. -SCBT QC
Date published: 2015-01-13
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