GFP Antibody (B-2) recommended for detection of GFP and GFP mutant fusion proteins by WB, IP, IF, FCM and ELISA

GFP Antibody (B-2): sc-9996

GFP Antibody (B-2) is rated 4.8 out of 5 by 130.
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Datasheets
    • GFP Antibody (B-2) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-238 representing full length GFP (green fluorescent protein) of Aequorea victoria origin
    • recommended for detection of GFP and GFP mutant fusion proteins by WB, IP, IF, FCM and ELISA
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to biotin for WB, IHC(P) and ELISA; and to either TRITC, PerCP, PerCP-Cy5.5 or Alexa Fluor® 405 for IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of GFP (B-2): sc-9996.
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Hola, Me gustaría saber qué significa la letra y el número que acompaña a vuestros anticuerpos (por ejemplo, anti-GFP B-2). Gracias

Asked by: Anonymous
Muchas gracias por su pregunta. La letra y el numero que acompañan nuestros anticuerpos representan el clon del hibridoma que se empleó para producir el anticuerpo (por ejemplo el anti-GFP). Por cualquier duda o consulta nuestro servicio tecnico está siempre a su disposición.
Answered by: Tech Support Europe
Date published: 2019-06-19

Does this antibody detect AcGFP1?

Asked by: user_na
Thank you for your question. Please contact our Technical Service Department for help with performing sequence analysis to predict if this antibody can detect your GFP protein of interest.
Answered by: Technical Support
Date published: 2019-06-16

请问此抗体的抗原决定簇大约在GFP 全长的哪一段氨基酸序列?

Asked by: DWLDWLDWL
感谢您的提问。sc-9996 GFP(B-2) 抗原的氨基酸序列是维多利亚多管发光水母来源的GFP的1-238个氨基酸。 Thank you for your inquiry. sc-9996 GFP(B-2) was raised against amino acids 1-238 representing full length GFP (green fluorescent protein) of Aequorea victoria origin.
Answered by: Technical Support 9
Date published: 2019-05-07

IS this antibody affinity purified or is it prot. A/G purified? IS there potential for Ms IgG contamination?

Asked by: DaveK
Thank you for your question. The exact method used to purify this mouse monoclonal antibody is considered proprietary information. Please contact our Technical Service Department for further support.
Answered by: Technical Support
Date published: 2019-03-21

Does this antibody detect Clover?

Asked by: nines
Thank you for your question. We do not have a protein sequence for Clover. If you can contact our Technical Service Department and provide a protein sequence for Clover then we can help perform sequence analysis of this GFP antibody's antigen to predict whether or not the antibody may be able to detect Clover.
Answered by: Technical Support
Date published: 2019-02-27

Do anti-GFP antibodies (GFP Antibody (B-2): sc-9996) recognize sfGFP? Thank you

Asked by: AleksTay
Thank you for your question. The sequence against which GFP Antibody (B-2): sc-9996 has been raised is not 100 % identical with the sequence of sfGFP. Since no epitope mapping has been performed for this antibody, we cannot say with absolute certainty whether it would recognize sfGFP. Please contact our technical service directly for more information or to arrange for a sample of this antibody to see if it is suitable for your application.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

Can we use sc9996 GFP antibody to detect Venus C-terminal in a western blot?

Asked by: VetR
Thank you for your question. There are a total of ten amino acids that differ between the amino acid sequence of Venus and the sequence of GFP against which this antibody has been raised. It is not possible to tell whether the antibody will recognize Venus or not. Please contact out technical service directly to arrange for a sample of the GFP antibody so you can try out whether the antibody would be suitable for your application.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

Hello, do you have any information whether this antibody cross-reacts with EYFP in a western blotting environment? Thank you.

Asked by: mautahu
Thank you for your question. We have not specifically evaluated GFP (B-2): sc-9996 against EYFP. sc-9996 was raised against amino acids 1-238 representing full length GFP (green fluorescent protein) of Aequorea victoria origin. The exact binding epitope has not been determined, yet. We have had positive feedback for the sc-9996 detecting various GFP and YFP variants. Thus, it seems to be likely this antibody might also detect this target at least in a WB experiment.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-30
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Rated 5 out of 5 by from Works great! It worked great for GFP expressed in plant tissues.
Date published: 2019-06-24
Rated 5 out of 5 by from Awesome This antibody is really awesome! I tried antibodies from three different companies and This one is by far the best! Clean signal, specific, easy to handle. Fantastic!
Date published: 2019-04-26
Rated 5 out of 5 by from IHC Mouse Brain Very good and clear staining on mouse brain tissue with IHC
Date published: 2019-02-26
Rated 5 out of 5 by from works better I bought this antibody several months ago and it works perfect my experiments
Date published: 2019-01-25
Rated 5 out of 5 by from Good for WB The GFP antibody (B-2) works well for western blot.
Date published: 2018-10-24
Rated 5 out of 5 by from Good GFP antibody This GFP antibody is also good for Arabidopsis in western blot.
Date published: 2018-09-19
Rated 3 out of 5 by from Efficenciy is very low in IP I used this antibody in IP to pulldown Gao-GFP from worm protein lysates but did not work well.
Date published: 2018-07-05
Rated 5 out of 5 by from Highly recommend this antibody! Very strong and specific staining in IHC. Very low background..... I compared it with other antibodies from different companies..... this was the very best.
Date published: 2018-06-11
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