GAPDH Antibody (G-9): sc-365062
- GAPDH Antibody (G-9) is a mouse monoclonal IgG1 κ, cited in 1,636 publications, provided at 200 µg/ml
- raised against amino acids 1-335 representing full length GAPDH of human origin
- Anti-GAPDH Antibody (G-9) is recommended for detection of GAPDH and GAPDH-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-GAPDH Antibody (G-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GAPDH Antibody (G-9) for WB and IHC(P) applications. These reagents are now offered in bundles with GAPDH Antibody (G-9) (see ordering information below).
QUICK LINKS
SEE ALSO...
GAPDH Antibody (G-9) is a mouse monoclonal IgG1 kappa light chain antibody raised against amino acids 1-335, representing the full-length glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein of human origin. Anti-GAPDH antibody (G-9) shows reactivity with mouse, rat, and human samples in various applications, including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). GAPDH is a key enzyme in glycolysis, catalyzing the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of NAD+ and inorganic phosphate, a crucial step in energy metabolism. Beyond its metabolic role, GAPDH has multiple non-glycolytic functions, including involvement in transcriptional activation, RNA binding, DNA repair, and nuclear RNA export. Notably, GAPDH undergoes post-translational modifications such as phosphorylation and nitrosylation, which regulate its diverse functions and subcellular localization. These modifications are essential during cellular processes like apoptosis, where GAPDH translocates to the nucleus. GAPDH is also implicated in neurodegenerative diseases by interacting with proteins such as β-amyloid precursor and huntingtin. GAPDH monoclonal antibody (G-9) is available in both unconjugated and various conjugated forms, including horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and Alexa Fluor® conjugates.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GAPDH Antibody (G-9) | sc-365062 | 200 µg/ml | $316.00 | |||
GAPDH Antibody (G-9): m-IgGκ BP-HRP Bundle | sc-522129 | 200 µg Ab, 40 µg BP | $354.00 | |||
GAPDH Antibody (G-9): m-IgG1 BP-HRP Bundle | sc-552050 | 200 µg Ab; 20 µg BP | $354.00 | |||
GAPDH Antibody (G-9) AC | sc-365062 AC | 500 µg/ml, 25% agarose | $416.00 | |||
GAPDH Antibody (G-9) HRP | sc-365062 HRP | 200 µg/ml | $316.00 | |||
GAPDH Antibody (G-9) FITC | sc-365062 FITC | 200 µg/ml | $330.00 | |||
GAPDH Antibody (G-9) PE | sc-365062 PE | 200 µg/ml | $343.00 | |||
GAPDH Antibody (G-9) Alexa Fluor® 488 | sc-365062 AF488 | 200 µg/ml | $357.00 | |||
GAPDH Antibody (G-9) Alexa Fluor® 546 | sc-365062 AF546 | 200 µg/ml | $357.00 | |||
GAPDH Antibody (G-9) Alexa Fluor® 594 | sc-365062 AF594 | 200 µg/ml | $357.00 | |||
GAPDH Antibody (G-9) Alexa Fluor® 647 | sc-365062 AF647 | 200 µg/ml | $357.00 | |||
GAPDH Antibody (G-9) Alexa Fluor® 680 | sc-365062 AF680 | 200 µg/ml | $357.00 | |||
GAPDH Antibody (G-9) Alexa Fluor® 790 | sc-365062 AF790 | 200 µg/ml | $357.00 |