Protein G PLUS-Agarose: sc-2002

Molecular purification methods such as immunoprecipitation (IP), co-IP and chromatin IP are important tools in studying lowly expressed proteins, protein-protein interactions or protein-nucleic acid interactions. Santa Cruz Biotechnology offers a comprehensive array of research support products for purification techniques. Protein G Agarose is a recommended specialty reagent used for isolating immunoglobulins from mammalian species. Protein G is an IgG binding protein expressed in Streptococcal bacteria much like Protein A but with differing binding specificities. Protein G binds to the Fc region of most mammalian immunoglobulins making it a powerful tool for antibody purification. Protein G Agarose is a recombinant Protein G type 3 that is linked to a high-quality Agarose resin, CL-6B, and together makes a superior and premium product which produces high purity and purification yield of IgGs from mammalian samples. Protein G Agarose is used with a wider range of IgG subclasses of human, mouse and rat serum than Protein A.

Protein G PLUS-Agarose References:

1. CM1 ligation initiates apoptosis in a caspase 8-dependent manner in Ramos cells and in a mitochondria-controlled manner in Raji cells.  |  Kim, D., et al. 2002. Hum Immunol. 63: 576-87. PMID: 12072193
2. Heterologous expression and purification of SpaB involved in subtilin biosynthesis.  |  Xie, L., et al. 2002. Biochem Biophys Res Commun. 295: 952-7. PMID: 12127987
3. KRIT1, a gene mutated in cerebral cavernous malformation, encodes a microtubule-associated protein.  |  Gunel, M., et al. 2002. Proc Natl Acad Sci U S A. 99: 10677-82. PMID: 12140362
4. Transient Activation and Tyrosine Phosphorylation of a Protein Kinase in Tobacco Cells Treated with a Fungal Elicitor.  |  Suzuki, K. and Shinshi, H. 1995. Plant Cell. 7: 639-647. PMID: 12242379
5. Analysis of the membrane topology for transmembrane domains 7-12 of the human reduced folate carrier by scanning cysteine accessibility methods.  |  Cao, W. and Matherly, LH. 2004. Biochem J. 378: 201-6. PMID: 14602046
6. Interleukin-17F signaling requires ubiquitination of interleukin-17 receptor via TRAF6.  |  Rong, Z., et al. 2007. Cell Signal. 19: 1514-20. PMID: 17346928
7. Tau binds both subunits of calcineurin, and binding is impaired by calmodulin.  |  Yu, DY., et al. 2008. Biochim Biophys Acta. 1783: 2255-61. PMID: 18639592
8. IL-17RD (Sef or IL-17RLM) interacts with IL-17 receptor and mediates IL-17 signaling.  |  Rong, Z., et al. 2009. Cell Res. 19: 208-15. PMID: 19079364
9. Targeted overexpression of EZH2 in the mammary gland disrupts ductal morphogenesis and causes epithelial hyperplasia.  |  Li, X., et al. 2009. Am J Pathol. 175: 1246-54. PMID: 19661437
10. Rictor regulates FBXW7-dependent c-Myc and cyclin E degradation in colorectal cancer cells.  |  Guo, Z., et al. 2012. Biochem Biophys Res Commun. 418: 426-32. PMID: 22285861
11. Transglutaminase Type 2 Regulates ER-Mitochondria Contact Sites by Interacting with GRP75.  |  D′Eletto, M., et al. 2018. Cell Rep. 25: 3573-3581.e4. PMID: 30590033
12. G protein beta gamma subunits stimulate phosphorylation of Shc adapter protein.  |  Touhara, K., et al. 1995. Proc Natl Acad Sci U S A. 92: 9284-7. PMID: 7568118
13. Role of p21 RAS in p210 bcr-abl transformation of murine myeloid cells.  |  Mandanas, RA., et al. 1993. Blood. 82: 1838-47. PMID: 7691239
14. Acute inflammatory injury in the lung precipitated by oxidant stress induces fibroblasts to synthesize and release transforming growth factor-alpha.  |  Vivekananda, J., et al. 1994. J Biol Chem. 269: 25057-61. PMID: 7929192