Date published: 2025-9-8

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YAP Anticorpo (G-6): sc-376830

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Fichas de dados
  • YAP Anticorpo G-6é um anticorpo monoclonal produzido em camundongo IgG1 κ YAP anticorpo, citado em 80 publicações, fornecido em 200 µg/ml
  • Produzido contra aminoácidos 206-330 mapeamento no C-terminus de YAP de human origem
  • YAP Anticorpo (G-6) é recomendado para a detecção de YAP de mouse, rat e human origem em WB, IP, IF, IHC(P) e ELISA
  • Anticorpo anti-YAP O anticorpo anti-YAP (G-6) está disponível conjugado com agarose para IP; HRP para WB, IHC(P) e ELISA; e com fioeritrina ou FITC para IF, IHC(P) e FCM
  • também disponível conjugado com Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 para WB (RGB), IF, IHC(P) e FCM, e para utilização com sistemas de imagiologia fluorescente RGB, tais como iBright™ FL1000, FluorChem™, Typhoon, Azure e outros sistemas comparáveis
  • também disponível conjugado com Alexa Fluor® 680 ou Alexa Fluor® 790 para WB (NIR), IF e FCM; para utilização com sistemas de deteção de infravermelhos próximos (NIR), tais como LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure e outros sistemas comparáveis
  • disponível como reagente TransCruz para ensaios de Gel Supershift e uso em ChIP (sc-376830 X, 200 µg/0.1 ml)
  • Entre em contato conosco e receba GRATUITAMENTE um amostra de 10 µg de YAP (G-6): sc-376830.
  • m-IgG Fc BP-HRP e m-IgG1 BP- HRP são os reagentes de deteção secundários preferidos para YAP Anticorpo (G-6) para aplicações WB and IHC(P). Estes reagentes são agora oferecidos em conjuntos com YAP Anticorpo (G-6) (ver informações para encomenda abaixo).

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VEJA TAMBÉM

O anticorpo YAP (G-6) é um anticorpo YAP monoclonal de camundongo IgG1 κ (também designado anticorpo YAP1) que detecta a proteína YAP de origem de camundongo, rato e humana por WB, IP, IF, IHC(P) e ELISA. O anticorpo YAP (G-6) está disponível na forma de anticorpo anti-YAP não conjugado, bem como em várias formas conjugadas de anticorpo anti-YAP, incluindo agarose, HRP, PE, FITC e vários conjugados Alexa Fluor®. A proteína associada ao YES, também conhecida como YAP, é uma molécula de ligação 14-3-3 que foi originalmente reconhecida em virtude de sua capacidade de se ligar ao domínio SH3 do YES. A ligação da YAP ao 14-3-3 requer a fosforilação de um resíduo de serina homólogo (Ser 112) no motivo de ligação YAP 14-3-3. As proteínas 14-3-3, altamente conservadas e expressas de forma ubíqua, regulam a diferenciação, a progressão do ciclo celular e a apoptose por meio da ligação de fosfoproteínas intracelulares envolvidas na transdução de sinais. A YAP pode vincular eventos na membrana plasmática e no citoesqueleto à inibição da transcrição no núcleo de uma maneira regulada pelas proteínas 14-3-3. A YAP compartilha homologia com o domínio WW do TAZ, coativador transcricional com motivo de ligação PDZ, que funciona como coativador transcricional ao se ligar ao motivo PPXY presente nos fatores de transcrição. A YAP é expressa em altos níveis no pulmão, na placenta, na próstata, no ovário e no testículo.

Para uso em exclusivo em pesquisa. Não se destina a uso em diagnostico e tratamento.

Alexa Fluor® é uma marca comercial da Molecular Probes Inc., OR., EUA

LI-COR® e Odyssey® são marcas registadas da LI-COR Biosciences

Informacoes sobre ordens

Nome do ProdutoNumero de CatalogoUNIDPrecoQdeFAVORITOS

YAP Anticorpo (G-6)

sc-376830
200 µg/ml
$316.00

Pacote do YAP (G-6): m-IgG Fc BP-HRP

sc-527550
200 µg Ab; 10 µg BP
$354.00

Pacote do YAP (G-6): m-IgG1 BP-HRP

sc-532923
200 µg Ab; 20 µg BP
$354.00

YAP Anticorpo (G-6) X

sc-376830 X
200 µg/0.1 ml
$316.00

YAP Anticorpo (G-6) AC

sc-376830 AC
500 µg/ml, 25% agarose
$416.00

YAP Anticorpo (G-6) HRP

sc-376830 HRP
200 µg/ml
$316.00

YAP Anticorpo (G-6) FITC

sc-376830 FITC
200 µg/ml
$330.00

YAP Anticorpo (G-6) PE

sc-376830 PE
200 µg/ml
$343.00

YAP Anticorpo (G-6) Alexa Fluor® 488

sc-376830 AF488
200 µg/ml
$357.00

YAP Anticorpo (G-6) Alexa Fluor® 546

sc-376830 AF546
200 µg/ml
$357.00

YAP Anticorpo (G-6) Alexa Fluor® 594

sc-376830 AF594
200 µg/ml
$357.00

YAP Anticorpo (G-6) Alexa Fluor® 647

sc-376830 AF647
200 µg/ml
$357.00

YAP Anticorpo (G-6) Alexa Fluor® 680

sc-376830 AF680
200 µg/ml
$357.00

YAP Anticorpo (G-6) Alexa Fluor® 790

sc-376830 AF790
200 µg/ml
$357.00

Hi, I'm working on IF staining of YAP in fibroblasts, what fixation buffer do you recommend for that with YAP Antibody (G-6): sc-376830? And how about the cell confluence? Will lower or higher confluence influence the YAP location in cells?

Asked by: pan16
Thank you for your question . We recommend using formalin for fixation and cell confluence varies from protein to protein and is determined by empirical data.
Answered by: BlakeJ
Date published: 2023-02-23

what is the certain antibody for rabbit YAP WB test (anti-YAP (1 : 1000; Santa Cruz, USA) in the study <Hydrostatic Pressure Modulates Intervertebral Disc Cell Survival and Extracellular Matrix Homeostasis via Regulating Hippo-YAP/TAZ Pathway>?

Asked by: Markhu
Thank you for your question. We don't know the certain antibody for YAP about this paper. If the author writes both clone number and protein name, then we can know the specific antibody. For YAP, we provide related products, please check the link below. https://www.scbt.com/search?Ntt=YAP1 感谢提问。 这篇文章中,我们并不知道,作者具体使用了我司哪个货号的抗体。 只有当作者同时写明了抗体的克隆号和蛋白名/基因名时,我们才知道具体的货号。 关于YAP,我们公司有如下的相关产品,请查看链接。 https://www.scbt.com/search?Ntt=YAP1
Answered by: Ling Chen
Date published: 2022-07-07

请问这个抗体是可以检测不同亚型的蛋白吗?他们的kd值是否有明显差别?

Asked by: grazylee
Thanks for your question. Anti-YAP1 Antibody (G-6) is recommended for detection of total YAP. Molecular Weight of YAP: 65 kDa. 感谢您的提问。Anti-YAP1 抗体(G-6) 推荐检测总 YAP ,YAP分子量是65kDa。
Answered by: Yanru Tao
Date published: 2022-06-09

Can this antibody be used in huaman BMMSCS samples in WB?

Asked by: AngelWang
Thank you for your question. We have not tested this antibody specifically on Bone Marrow Mesenchymal Stem Cells, so I am unable to specifically say if the YAP protein is expressed in these cells. We recommend you research in the primary literature to see if these BMMSCS cells express the YAP protein.
Answered by: Tech Service
Date published: 2019-12-02

Has anyone tried this anybody for ChIP? Our lab used the predecessor antibody (now discontinued) for ChIP and I need to do it again looking for some other binding sites.

Asked by: Hope34Me
Thank you for your question. We are not currently aware of any data or product citations using this antibody for ChIP research applications. We do offer an alternative antibody, YAP Antibody (63.7): sc-101199, which has been published in ChIP research applications.
Answered by: Technical Support
Date published: 2019-06-16

Is antigen retrieval recommended for this antibody in IHC? If so, are there any guidelines about which antigen retrieval methods are best for the protein?

Asked by: annag16
Thank you for your question. We recommend antigen retrieval for this antibody, sodium citrate with heat or EDTA are the preferred methods. You can find the recommended IHC protocol here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Tech Service
Date published: 2019-01-14

Hello, I'm interested in getting  a FREE 10 µg sample of YAP (G-6): sc-376830. We are working with the mouse intestinal organoids and would like to detect how YAP protein localisation changes during oncoprotein activation. I would appreciate your reply.

Asked by: hui99
Thank you for your question. If you have an interest in testing a free 10 µg sample of sc-376830, please contact our Technical Service by phone or email or by Live Chat.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-21

Can this mouse monoclonal antibody be used to stain mouse samples in IF and IHC?

Asked by: AbPolly
Thank you for your question. Yes, this antibody is recommended for use in IF and IHC with mouse samples. We recommend using a directly conjugated version of the antibody. This YAP antibody, sc-376830, is available conjugated to HRP, PE, FITC, AF488 and AF647.
Answered by: Technical Support 14
Date published: 2017-01-20
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Rated 5 out of 5 by from Good antibody for WBThe antibody showed great reactivity in WB using several human cell lines.
Date published: 2024-08-23
Rated 4 out of 5 by from Good.YAP antibody detected reasonably in human stromal cells by WB, dilution 1:500.
Date published: 2022-04-18
Rated 5 out of 5 by from Great antibody!It works well in immunofluorescence. 1:100 dilution
Date published: 2021-10-21
Rated 1 out of 5 by from the batch A2220 does not work at all!the batch A2220 does not work at all! Gives a band at the wrong size lower than 60Kda, and a strong laddering above. Another batch of the same ref did work with my samples.
Date published: 2020-12-10
Rated 5 out of 5 by from Good at WB/IFNice antibody for Immunofluorescence and western blotting!
Date published: 2020-11-30
Rated 4 out of 5 by from 功能很出色我一个月前购买此产品,并为此感到高兴,之后有需要的话会再购买,主要是抗体浓度需要提高到很高希望能在这方面加以改善,谢谢
Date published: 2020-10-22
Rated 5 out of 5 by from Nice and single band (w.b.)Produced a single band at the expected molecular weight (~65kDa). Sample: MDA-MB-231 wcl (30ug). Blocking: 1h in blocking buffer (0.05% tween-20 in TBS with 5% skin milk). Primary antibody: 1:200 in blocking buffer, overnight @ 4oC. Secondary antibody: 1:3000 in blocking buffer. Exposure time: 30 seconds* *further dilution of the primary antibody (1:400-1:500 ?) may yield similar results at the expense of a prolonged exposure time (2-4 minutes).
Date published: 2017-11-18
Rated 5 out of 5 by from Good antibody!I used this antibody for WB. I detected a band at 65 kDa. I'm satisfied with the antibody.
Date published: 2017-11-06
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YAP Anticorpo (G-6) is rated 4.7 out of 5 by 30.
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