Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
von Willebrand Factor/VWF CRISPR/Cas9 KO Plasmid (h): sc-400217
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- von Willebrand Factor/VWF CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the von Willebrand Factor/VWF genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: von Willebrand Factor/VWF Antibody (C-12): sc-365712
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
von Willebrand Factor/VWF CRISPR/Cas9 KO Plasmid (h) | sc-400217 | 20 µg | $397.00 |
Overview
VWF encodes von Willebrand factor, a large multimeric glycoprotein synthesized primarily by endothelial cells and megakaryocytes and stored in Weibel–Palade bodies and platelet α-granules. VWF mediates platelet tethering and adhesion to exposed subendothelial collagen under high shear stress and stabilizes coagulation factor VIII in circulation, linking primary hemostasis with the coagulation cascade. Its regulated secretion and multimer processing by ADAMTS13 influence thrombus formation dynamics and vascular homeostasis. Dysregulated VWF abundance, multimer composition, or clearance is associated with inherited and acquired bleeding and thrombotic phenotypes, making it a key node for studying endothelial biology and hemostatic balance.
von Willebrand Factor/VWF CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the VWF gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the VWF together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the VWF open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish von Willebrand Factor/VWF protein expression.
This CRISPR knockout system enables efficient generation of VWF-deficient cell models for investigation of von Willebrand Factor/VWF signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting VWF exon(s) critical for von Willebrand Factor/VWF function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple VWF genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by von Willebrand Factor/VWF CRISPR/Cas9 KO Plasmid (h) and von Willebrand Factor/VWF CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the VWF locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by von Willebrand Factor/VWF HDR Plasmid (h) and von Willebrand Factor/VWF HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by VWF homology arms to support homology-directed repair at defined VWF target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.