TIGAR Antibody (M2-P4H2): sc-101393
- TIGAR Antibody (M2-P4H2) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against a synthetic peptide corresponding to amino acids 239-248 of TIGAR of human origin
- recommended for detection of TIGAR of human origin by WB, IF and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for TIGAR Antibody (M2-P4H2). This work is in progress.
QUICK LINKS
SEE ALSO...
TIGAR Antibody (M2-P4H2) is a mouse monoclonal IgG1 antibody that detects TIGAR (TP53-induced glycolysis and apoptosis regulator) in human samples through applications such as western blotting (WB), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TIGAR, also known as C12orf5, is a 270 amino acid protein that plays a crucial role in cellular metabolism and stress response by modulating glycolysis and the pentose phosphate pathway. Induced by the p53 tumor suppressor pathway, TIGAR helps protect cells from oxidative stress by lowering intracellular levels of fructose-2,6-bisphosphate, which in turn inhibits glycolysis and redirects metabolic flux towards the pentose phosphate shunt. This metabolic shift is vital as it not only reduces the production of reactive oxygen species, which can cause DNA damage and trigger apoptosis, but also enhances the generation of NADPH, a critical cofactor for biosynthetic reactions and antioxidant defense. The ability of TIGAR to regulate these pathways underscores its importance in maintaining cellular homeostasis and its potential implications in cancer biology, where p53 mutations can disrupt these protective mechanisms. Anti-TIGAR antibody (M2-P4H2) serves as an essential tool for researchers investigating the intricate roles of TIGAR in cellular metabolism and stress responses.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TIGAR Antibody (M2-P4H2) References:
- TIGAR, a p53-inducible regulator of glycolysis and apoptosis. | Bensaad, K., et al. 2006. Cell. 126: 107-20. PMID: 16839880
- The diverse role of TIGAR in cellular homeostasis and cancer. | Geng, J., et al. 2018. Free Radic Res. 52: 1240-1249. PMID: 30284488
- Structure, regulation, and biological functions of TIGAR and its role in diseases. | Tang, J., et al. 2021. Acta Pharmacol Sin. 42: 1547-1555. PMID: 33510458
- TIGAR drives colorectal cancer ferroptosis resistance through ROS/AMPK/SCD1 pathway. | Liu, MY., et al. 2022. Free Radic Biol Med. 182: 219-231. PMID: 35271998
- TIGAR alleviates oxidative stress in brain with extended ischemia via a pentose phosphate pathway-independent manner. | Liu, M., et al. 2022. Redox Biol. 53: 102323. PMID: 35576689
- Nuclear TIGAR mediates an epigenetic and metabolic autoregulatory loop via NRF2 in cancer therapeutic resistance. | Wang, H., et al. 2022. Acta Pharm Sin B. 12: 1871-1884. PMID: 35847493
- TIGAR deficiency induces caspase-1-dependent trophoblasts pyroptosis through NLRP3-ASC inflammasome. | Guo, J., et al. 2023. Front Immunol. 14: 1114620. PMID: 37122710
- TIGAR reduces neuronal ferroptosis by inhibiting succinate dehydrogenase activity in cerebral ischemia. | Wang, X., et al. 2024. Free Radic Biol Med. 216: 89-105. PMID: 38494143
- TIGAR exacerbates obesity by triggering LRRK2-mediated defects in macroautophagy and chaperone-mediated autophagy in adipocytes. | Zhang, T., et al. 2024. Autophagy. 20: 1741-1761. PMID: 38686804
- Disruption of TIGAR-TAK1 alleviates immunopathology in a murine model of sepsis. | Wang, D., et al. 2024. Nat Commun. 15: 4340. PMID: 38773142
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TIGAR Antibody (M2-P4H2) | sc-101393 | 200 µg/ml | $322.00 |