Date published: 2025-9-7

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Sox9 Anticorpo (E-9): sc-166505

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Fichas de dados
  • Sox9 Anticorpo E-9é um anticorpo monoclonal produzido em camundongo IgG2a κ Sox9 anticorpo, citado em 79 publicações, fornecido em 200 µg/ml
  • específico para um epítopo mapeado entre os aminoácidos 36-55 no N-terminus de Sox-9 de human origem
  • Sox9 Anticorpo (E-9) é recomendado para a detecção de Sox-9 de mouse, rat, human e avian de origem WB, IP, IF e ELISA; Reage também com outras espécies, incluindo and equine, canine, bovine, porcine and avian
  • Anticorpo anti-Sox9 O anticorpo anti-Sox9 (E-9) está disponível conjugado com agarose para IP; HRP para WB, IHC(P) e ELISA; e com fioeritrina ou FITC para IF, IHC(P) e FCM
  • também disponível conjugado com Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 para WB (RGB), IF, IHC(P) e FCM, e para utilização com sistemas de imagiologia fluorescente RGB, tais como iBright™ FL1000, FluorChem™, Typhoon, Azure e outros sistemas comparáveis
  • também disponível conjugado com Alexa Fluor® 680 ou Alexa Fluor® 790 para WB (NIR), IF e FCM; para utilização com sistemas de deteção de infravermelhos próximos (NIR), tais como LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure e outros sistemas comparáveis
  • disponível como reagente TransCruz para ensaios de Gel Supershift e uso em ChIP (sc-166505 X, 200 µg/0.1 ml)
  • Entre em contato conosco e receba GRATUITAMENTE um amostra de 10 µg de Sox9 (E-9): sc-166505.
  • m-IgG Fc BP-HRP, 2a BP-HRP">m-IgG2a BP-HRP e m-IgGκ BP-HRP são os reagentes de deteção secundários preferidos para Sox9 Antibody (E-9) para aplicações WB. Estes reagentes são agora oferecidos em pacotes com Sox9 Antibody (E-9)(ver informações de encomenda abaixo).

LINKS RÁPIDOS

VEJA TAMBÉM

O anticorpo Sox-9 (E-9) é um anticorpo Sox-9 monoclonal de camundongo IgG2a κ (também designado anticorpo SOX9 ou anticorpo do fator de transcrição HMG-box 9 relacionado a SRY) que detecta a proteína Sox-9 de origem de camundongo, rato e humana por WB, IP, IF e ELISA. O anticorpo Sox-9 (E-9) está disponível na forma de anticorpo anti-Sox-9 não conjugado, bem como em várias formas conjugadas de anticorpo anti-Sox-9, incluindo agarose, HRP, PE, FITC e vários conjugados Alexa Fluor®. Os genes Sox compreendem uma família de genes relacionados ao gene SRY de determinação do sexo em mamíferos. Esses genes contêm, de forma semelhante, sequências que codificam o domínio HMG-box, responsável pela atividade de ligação ao DNA específica da sequência. Os genes Sox codificam reguladores de transcrição putativos implicados na decisão do destino das células durante o desenvolvimento e no controle de diversos processos de desenvolvimento. O grupo altamente complexo de genes Sox agrupa pelo menos 40 loci diferentes que divergem rapidamente em várias linhagens de animais. Atualmente, 30 genes Sox foram identificados. Foi demonstrado que os membros dessa família são conservados durante a evolução e desempenham funções importantes durante o desenvolvimento animal. Alguns estão envolvidos em doenças humanas, incluindo a inversão de sexo.

Para uso em exclusivo em pesquisa. Não se destina a uso em diagnostico e tratamento.

Alexa Fluor® é uma marca comercial da Molecular Probes Inc., OR., EUA

LI-COR® e Odyssey® são marcas registadas da LI-COR Biosciences

Referencias do Sox9 Anticorpo (E-9):

  1. Identificação de um novo gene relacionado com Sry e a sua expressão específica em células germinativas.  |  Osaki, E., et al. 1999. Nucleic Acids Res. 27: 2503-10. PMID: 10359848
  2. Papel dos factores Sox na determinação neural: sinalização conservada na evolução?  |  Sasai, Y. 2001. Int J Dev Biol. 45: 321-6. PMID: 11291862
  3. Ancestralidade e diversidade da superfamília das caixas HMG.  |  Laudet, V., et al. 1993. Nucleic Acids Res. 21: 2493-501. PMID: 8506143
  4. Sox10, um novo modulador transcricional em células gliais.  |  Kuhlbrodt, K., et al. 1998. J Neurosci. 18: 237-50. PMID: 9412504
  5. Caracterização e mapeamento do gene humano SOX14.  |  Arsic, N., et al. 1998. Cytogenet Cell Genet. 83: 139-46. PMID: 9925951

Informacoes sobre ordens

Nome do ProdutoNumero de CatalogoUNIDPrecoQdeFAVORITOS

Sox9 Anticorpo (E-9)

sc-166505
200 µg/ml
$316.00

Pacote do Sox9 (E-9): m-IgG Fc BP-HRP

sc-528983
200 µg Ab; 10 µg BP
$354.00

Pacote do Sox9 (E-9): m-IgGκ BP-HRP

sc-521635
200 µg Ab, 40 µg BP
$354.00

Pacote do Sox9 (E-9): m-IgG2a BP-HRP

sc-547225
200 µg Ab; 10 µg BP
$354.00

Sox9 Anticorpo (E-9) X

sc-166505 X
200 µg/0.1 ml
$316.00

Sox9 Anticorpo (E-9) AC

sc-166505 AC
500 µg/ml, 25% agarose
$416.00

Sox9 Anticorpo (E-9) HRP

sc-166505 HRP
200 µg/ml
$316.00

Sox9 Anticorpo (E-9) FITC

sc-166505 FITC
200 µg/ml
$330.00

Sox9 Anticorpo (E-9) PE

sc-166505 PE
200 µg/ml
$343.00

Sox9 Anticorpo (E-9) Alexa Fluor® 488

sc-166505 AF488
200 µg/ml
$357.00

Sox9 Anticorpo (E-9) Alexa Fluor® 546

sc-166505 AF546
200 µg/ml
$357.00

Sox9 Anticorpo (E-9) Alexa Fluor® 594

sc-166505 AF594
200 µg/ml
$357.00

Sox9 Anticorpo (E-9) Alexa Fluor® 647

sc-166505 AF647
200 µg/ml
$357.00

Sox9 Anticorpo (E-9) Alexa Fluor® 680

sc-166505 AF680
200 µg/ml
$357.00

Sox9 Anticorpo (E-9) Alexa Fluor® 790

sc-166505 AF790
200 µg/ml
$357.00

Sox9 (E-9) peptídeo neutralizante

sc-166505 P
100 µg/0.5 ml
$68.00

I would like to know at which dilution this antibody Sox9/647 (sc-166505) is used for immunofluorescence assays. Thanks, Jordi

Asked by: Jordi
Santa Cruz Biotechnology tests at a 1:50 dilution for Immunofluorescence.
Answered by: Technical Service
Date published: 2024-05-07

Is this product appropriate for flow cytometry? Have you got citations of people successfully using it?

Asked by: ailsaw
Thank you for your question. Fluorescent conjugates of this antibody can be used for flow cytometry. As of yet, we have not received any published data on flow cytometry use.
Answered by: Tech Support Europe
Date published: 2023-09-22

where is the band area, why mine is at 40~,

Asked by: minch
Thank you for your question. Please contact our Technical Service Department for more information. Our Asian Technical Service team is available at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Jiajun Z
Date published: 2023-07-27

Can the conjugated version of this antibody (546) be used in combination with other secondary antibodies for double-staining? I haven't used many mouse-on-mouse stains and I know that can be problematic but mouse is listed under reactive species.

Asked by: Anonymous
Thank you for your question. We do not recommend using a secondary antibody or binding protein with a primary antibody that is already conjugated. Steric hindrance may negatively influence your results. Most samples and tissues are unproblematic when using a mouse primary antibody and anti-mouse secondary detection. When working with tissues make sure to remove all blood from the sample to prevent the secondary from picking up endogenous IgG. Using a direct conjugate (without a secondary) will circumvent this issue entirely.
Answered by: Technical Support Europe
Date published: 2020-10-28

Has anyone tried with IHC before? I have got mice testes samples which are paraffin fixed. I tried doing it but there isn't any signal.

Asked by: Evaa
Thank you for your question. We have not yet tested this antibody in formalin-fixed paraffin-embedded tissue sections, and so IHC(P) is not a recommended application at this time. We have successfully used the antibody on formalin-fixed cells for IF. In addition, there are several publications citing use of this antibody for IHC staining. For example, PMID's: 25917841 and 30244259.
Answered by: Technical Support
Date published: 2019-02-26

Is this product acceptable for ChIP/ChIP-seq applications?

Asked by: che183
This antibody has not yet been tested in the ChIP or ChIP-seq applications.
Answered by: Technical Support
Date published: 2018-12-21

I used this antibody for IF in rat cartilage cryosections but no staining was observed. Could you please recommend a protocol for this? Which is the best fixative and permeabilization agent? Is antigen retrieval necessary? Thanks.

Asked by: sox9
Thank you for your question. I have provided a link to our recommended protocol for immunofluorescent staining of frozen tissue sections. We recommend fixing slides in cold acetone for 10 minutes. A permeabilization agent should not be necessary if fixing in acetone. If you are using a formalin-based fixative, then permeabilization with Triton X-100 may be needed. I would also recommend reviewing the methods in the following publication on PubMed (PMID: 25917841) which cites use of this Sox-9 Antibody (E-9): sc-166505 for IHC in rat tissue. https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-01-13

What is the recommended fixation method for IF with this Sox-9 (E-9): sc-166505?

Asked by: cjMara
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10ºC methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support 9
Date published: 2017-01-11
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Rated 1 out of 5 by from Poor astrocyte specificityUsed this antibody for astrocyte-specific labeling and it labeled both neurons and astrocytes. Very bad specificity. IHC 1:200 and 1:1000 concentrations, both had very bad astrocyte labeling.
Date published: 2025-03-13
Rated 1 out of 5 by from Did not work well for WBThis Ab did not work well for neonatal mouse lungs for WB.
Date published: 2024-12-10
Rated 5 out of 5 by from First class service.Express delivery, proper documentation, perfect quality.
Date published: 2022-07-28
Rated 5 out of 5 by from 功能很出色,发货速度快,技术支持很给力!客服答复很快,供货稳定,发货速度快,说明很详细,使用效果很好,技术支持也很给力!会推荐给同事,后期需要还会购买!
Date published: 2022-07-04
Rated 5 out of 5 by from CorrectoFunciona correctamente, dentro de los estandares establecidos
Date published: 2021-08-23
Rated 4 out of 5 by from 不错产品不错, 值得下一次购买哦.....................................
Date published: 2021-08-09
Rated 5 out of 5 by from SOX9 antibodyExcellent antibody for immunofluorescence and immunohistochemistry. Dilution: 1 to 1000. Tested in intestinal tissue
Date published: 2021-03-18
Rated 3 out of 5 by from IF(free sample) used for IF for characterization of tumor cells, No problem for signal detection
Date published: 2020-10-06
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Sox9 Anticorpo (E-9) is rated 4.1 out of 5 by 34.
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