SARA Antibody (F-9): sc-133071

SARA Antibody (F-9) is a mouse monoclonal IgG1 antibody that detects SARA in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SARA, also known as NSP, MADHIP, SMADIP, or ZFYVE9, is a double zinc finger protein characterized by its FYVE domain, which plays a crucial role in the recruitment of Smad proteins to the TGF-beta receptor, ensuring proper subcellular localization of the activated receptor-bound complex. The FYVE domain directs SARA to early endosomal compartments, where SARA interacts with TGF-beta receptors and Smads, facilitating the assembly of signaling complexes essential for TGF-beta signaling pathways. Notably, SARA′s interaction with the promyelocytic leukemia (PML) tumor suppressor enhances the association and accumulation of SARA and the TGF-beta receptor within early endosomes, thereby promoting effective signaling. Additionally, SARA is instrumental in recruiting protein phosphatase 1 catalytic subunit (PP1c) to the Smad7-GADD34 complex, which is vital for the dephosphorylation of the TGF-beta receptor by Smad7, a key mechanism for regulating negative feedback in TGF-beta signaling. anti-SARA antibody (F-9) is an invaluable tool for researchers studying TGF-beta signaling and its implications in various biological processes and diseases.

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SARA Antibody (F-9) References:

1. The FYVE domain in Smad anchor for receptor activation (SARA) is sufficient for localization of SARA in early endosomes and regulates TGF-beta/Smad signalling.  |  Itoh, F., et al. 2002. Genes Cells. 7: 321-31. PMID: 11918675
2. The role of internalization in transforming growth factor beta1-induced Smad2 association with Smad anchor for receptor activation (SARA) and Smad2-dependent signaling in human mesangial cells.  |  Runyan, CE., et al. 2005. J Biol Chem. 280: 8300-8. PMID: 15613484
3. Role of SARA (SMAD anchor for receptor activation) in maintenance of epithelial cell phenotype.  |  Runyan, CE., et al. 2009. J Biol Chem. 284: 25181-9. PMID: 19620243
4. Smad anchor for receptor activation (SARA) in TGF-beta signaling.  |  Tang, WB., et al. 2010. Front Biosci (Elite Ed). 2: 857-60. PMID: 20515759
5. Phosphatidylinositol 3-kinase and Rab5 GTPase inversely regulate the Smad anchor for receptor activation (SARA) protein independently of transforming growth factor-β1.  |  Runyan, CE., et al. 2012. J Biol Chem. 287: 35815-24. PMID: 22942286
6. SARA and RNF11 at the crossroads of EGFR signaling and trafficking.  |  Kostaras, E., et al. 2014. Methods Enzymol. 535: 225-47. PMID: 24377927
7. The FYVE domain of Smad Anchor for Receptor Activation (SARA) is required to prevent skin carcinogenesis, but not in mouse development.  |  Chang, HM., et al. 2014. PLoS One. 9: e105299. PMID: 25170969
8. New Player in Endosomal Trafficking: Differential Roles of Smad Anchor for Receptor Activation (SARA) Protein.  |  Rozés-Salvador, V., et al. 2018. Mol Cell Biol. 38: PMID: 30275343
9. SMURF2 and SMAD7 induce SARA degradation via the proteasome.  |  Wojtowicz, S., et al. 2020. Cell Signal. 72: 109627. PMID: 32283253
10. SARA suppresses myofibroblast precursor transdifferentiation in fibrogenesis in a mouse model of scleroderma.  |  Corano Scheri, K., et al. 2022. JCI Insight. 7: PMID: 36136606

The preferred SARA antibody is SARA Antibody (B-9): sc-74493.