SA-1 Antibody (F-3): sc-271976

SA-1 Antibody (F-3) is a mouse monoclonal IgG2a antibody that detects SA-1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SA-1, also known as stromal antigen 1 or STAG1, is a crucial 1258 amino acid component of the cohesion complex, which plays a vital role in ensuring proper cohesion of sister chromatids following DNA replication. SA-1 is primarily localized in the nucleus, where SA-1 associates with chromatin, facilitating maintenance of chromatid cohesion during cell division. Notably, SA-1 undergoes phosphorylation by polo-like kinase (Plk), a post-translational modification that triggers SA-1′s dissociation from chromatin, allowing accurate separation of chromosomes during anaphase. Errors in chromatid separation can lead to aneuploidy and various diseases, including cancer. SA-1 is expressed in key hematopoietic tissues such as thymus, bone marrow, and spleen, and exhibits 99% similarity to SA-1′s mouse counterpart, underscoring evolutionary conservation and significance in cellular processes. anti-SA-1 antibody (F-3) effectively detects SA-1 across multiple species and applications, making this antibody an invaluable tool for researchers studying chromosomal dynamics and related cellular mechanisms.

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SA-1 Antibody (F-3) References:

1. EGF- and cell-cycle-regulated STAG1/PMEPA1/ERG1.2 belongs to a conserved gene family and is overexpressed and amplified in breast and ovarian cancer.  |  Giannini, G., et al. 2003. Mol Carcinog. 38: 188-200. PMID: 14639658
2. Purification, molecular cloning, and sequencing of salivary cystatin SA-1.  |  Al-Hashimi, I., et al. 1988. J Biol Chem. 263: 9381-7. PMID: 2837486
3. N6-Methyladenosine Modification of Fatty Acid Amide Hydrolase Messenger RNA in Circular RNA STAG1-Regulated Astrocyte Dysfunction and Depressive-like Behaviors.  |  Huang, R., et al. 2020. Biol Psychiatry. 88: 392-404. PMID: 32387133
4. Whole Exome Sequencing Identifies Cohesin Component STAG1 Mutation in de novo Acute Myeloid Leukemia (FAB M2): A Pilot Study with Cytogenetics, Clinical and Prognostic Implications.  |  Sakthivel, KM., et al. 2021. J Environ Pathol Toxicol Oncol. 40: 51-64. PMID: 33639073
5. Novel STAG1 Frameshift Mutation in a Patient Affected by a Syndromic Form of Neurodevelopmental Disorder.  |  Di Muro, E., et al. 2021. Genes (Basel). 12: PMID: 34440290
6. Potential role of STAG1 mutations in genetic predisposition to childhood hematological malignancies.  |  Saitta, C., et al. 2022. Blood Cancer J. 12: 88. PMID: 35654786
7. A Novel De Novo STAG1 Variant in Monozygotic Twins with Neurodevelopmental Disorder: New Insights in Clinical Heterogeneity.  |  Cipriano, L., et al. 2024. Genes (Basel). 15: PMID: 39336775
8. SA-1 antigen expression in small intensely fluorescent cells is associated with proliferation.  |  Sejvar, JJ., et al. 1993. Dev Biol. 157: 547-52. PMID: 8099047
9. Modification of TNF-alpha pharmacokinetics in SA-1 tumor-bearing mice.  |  Kus, B., et al. 1993. Int J Cancer. 55: 110-4. PMID: 8344743
10. SA-1, a nuclear protein encoded by one member of a novel gene family: molecular cloning and detection in hemopoietic organs.  |  Carramolino, L., et al. 1997. Gene. 195: 151-9. PMID: 9305759

The preferred SA-1 antibody is SA-1 Antibody (A-9): sc-365061.