Date published: 2025-9-9

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CD31/PECAM-1 Anticorpo (H-3): sc-376764

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Fichas de dados
  • CD31/PECAM-1 Anticorpo H-3é um anticorpo monoclonal produzido em camundongo IgG1 κ CD31/PECAM-1 anticorpo, citado em 231 publicações, fornecido em 200 µg/ml
  • específico para um epítopo mapeado entre os aminoácidos699-727 no C-terminus de PECAM-1 de mouse origem
  • CD31/PECAM-1 Anticorpo (H-3) é recomendado para a detecção de PECAM-1 de mouse, rat e human origem em WB, IP, IF, IHC(P) e ELISA
  • Anticorpo anti-CD31/PECAM-1 O anticorpo anti-CD31/PECAM-1 (H-3) está disponível conjugado com agarose para IP; HRP para WB, IHC(P) e ELISA; e com fioeritrina ou FITC para IF, IHC(P) e FCM
  • também disponível conjugado com Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 para WB (RGB), IF, IHC(P) e FCM, e para utilização com sistemas de imagiologia fluorescente RGB, tais como iBright™ FL1000, FluorChem™, Typhoon, Azure e outros sistemas comparáveis
  • também disponível conjugado com Alexa Fluor® 680 ou Alexa Fluor® 790 para WB (NIR), IF e FCM; para utilização com sistemas de deteção de infravermelhos próximos (NIR), tais como LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure e outros sistemas comparáveis
  • Entre em contato conosco e receba GRATUITAMENTE um amostra de 10 µg de CD31/PECAM-1 (H-3): sc-376764.
  • m-IgG Fc BP-HRP e m-IgG1 BP- HRP são os reagentes de deteção secundários preferidos para CD31/PECAM-1 Anticorpo (H-3) para aplicações WB and IHC(P). Estes reagentes são agora oferecidos em conjuntos com CD31/PECAM-1 Anticorpo (H-3) (ver informações para encomenda abaixo).

LINKS RÁPIDOS

VEJA TAMBÉM

O Anticorpo PECAM-1 (H-3) é um anticorpo monoclonal de camundongo IgG1 κ PECAM-1 (também designado anticorpo CD31 ou anticorpo endoCAM) que detecta a proteína PECAM-1 de origem de camundongo, rato e humana por WB, IP, IF, IHC(P) e ELISA. O anticorpo PECAM-1 (H-3) está disponível na forma de anticorpo anti-PECAM-1 não conjugado, bem como em várias formas conjugadas de anticorpo anti-PECAM-1, incluindo agarose, HRP, PE, FITC e vários conjugados Alexa Fluor®. As moléculas de adesão celular são uma família de glicoproteínas de superfície celular intimamente relacionadas, envolvidas nas interações célula-célula durante o crescimento, e acredita-se que desempenhem um papel importante na embriogênese e no desenvolvimento. A expressão da molécula de adesão celular neuronal (NCAM) é observada em vários tumores humanos, incluindo neuroblastomas, rabdomiossarcomas, tumores de Wilms, sarcomas de Ewing e algumas malignidades mieloides primitivas. A molécula de adesão intracelular-1 (ICAM-1), também conhecida como CD54, é uma proteína de membrana integral da superfamília da imunoglobulina e reconhece as integrinas β2/α1 e β2/αM. PECAM-1 (molécula de adesão de plaquetas/células endoteliais-1), também conhecida como CD31, é uma glicoproteína expressa nas superfícies celulares de monócitos, neutrófilos, plaquetas e uma subpopulação de células T. A VCAM-1 (molécula de adesão de células vasculares-1) foi identificada pela primeira vez como uma molécula de adesão induzida em células endoteliais humanas por citocinas inflamatórias, como IL-1, fator de necrose tumoral (TNF) e lipopolissacarídeo (LPS). O gene KALIG codifica uma proteína semelhante à molécula de adesão de células nervosas (NCAM) e é deletado em 66% dos pacientes com síndrome de Kallmann, anosmia com hipogonadismo secundário.

Para uso em exclusivo em pesquisa. Não se destina a uso em diagnostico e tratamento.

Alexa Fluor® é uma marca comercial da Molecular Probes Inc., OR., EUA

LI-COR® e Odyssey® são marcas registadas da LI-COR Biosciences

Informacoes sobre ordens

Nome do ProdutoNumero de CatalogoUNIDPrecoQdeFAVORITOS

CD31/PECAM-1 Anticorpo (H-3)

sc-376764
200 µg/ml
$316.00

Pacote do CD31/PECAM-1 (H-3): m-IgG Fc BP-HRP

sc-527547
200 µg Ab; 10 µg BP
$354.00

Pacote do CD31/PECAM-1 (H-3): m-IgG1 BP-HRP

sc-532920
200 µg Ab; 20 µg BP
$354.00

CD31/PECAM-1 Anticorpo (H-3) AC

sc-376764 AC
500 µg/ml, 25% agarose
$416.00

CD31/PECAM-1 Anticorpo (H-3) HRP

sc-376764 HRP
200 µg/ml
$316.00

CD31/PECAM-1 Anticorpo (H-3) FITC

sc-376764 FITC
200 µg/ml
$330.00

CD31/PECAM-1 Anticorpo (H-3) PE

sc-376764 PE
200 µg/ml
$343.00

CD31/PECAM-1 Anticorpo (H-3) Alexa Fluor® 488

sc-376764 AF488
200 µg/ml
$357.00

CD31/PECAM-1 Anticorpo (H-3) Alexa Fluor® 546

sc-376764 AF546
200 µg/ml
$357.00

CD31/PECAM-1 Anticorpo (H-3) Alexa Fluor® 594

sc-376764 AF594
200 µg/ml
$357.00

CD31/PECAM-1 Anticorpo (H-3) Alexa Fluor® 647

sc-376764 AF647
200 µg/ml
$357.00

CD31/PECAM-1 Anticorpo (H-3) Alexa Fluor® 680

sc-376764 AF680
200 µg/ml
$357.00

CD31/PECAM-1 Anticorpo (H-3) Alexa Fluor® 790

sc-376764 AF790
200 µg/ml
$357.00

CD31/PECAM-1 (H-3) peptídeo neutralizante

sc-376764 P
100 µg/0.5 ml
$68.00

buongiorno, desidererei sapere quale secondario usare con questo anticorpo

Asked by: AlePri
Thank you for your question. CD31/PECAM-1 Antibody (H-3) is a mouse monoclonal IgG1 κ antibody that can be detected using any of our anti-kappa, anti-Fc, or anti-IgG1 secondary antibodies. Please contact technical service for further information on what would be suitable for your particular experimental setup.
Answered by: Tech Support Europe
Date published: 2025-04-02

What is the suitable antigen retrieval method for this antibody?

Asked by: DiVa03
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext .1, allowing for a more interactive discussion of this and other related questions
Answered by: Blake J
Date published: 2021-09-27

Are trial samples for this antibody offered?

Asked by: james001
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

Hola, para este anticuerpo es mejor para bloqueo leche, BSA %??, y que porcentaje de tween? y para lavar, PBS o TBS? Hello, for this antibody is better for blocking milk, BSA, %?? and what percentage of tween? and to wash, PBS or TBS?

Asked by: MARIACONSUELO
Thank you for your question. We recommend blocking with 5% milk or 3% BSA with 0.05% Tween-20 for Western blot. We wash with TBS but PBS would work to. If you have additional questions, please contact Technical Support at 1800-457-3801 option 2, by emailing scbt@scbt.com or by live chat on our website at www.scbt.com so we may further assist you.
Answered by: Santa Cruz TS
Date published: 2021-07-07

Hi, can i get a testing aliquot of this antibody for western blot? I need to examine PECAM protein level in human endothelial cells and would like to try out the antibody before we purchase a whole vial. 

Asked by: Yitong
Thank you for your question. Please get in touch with our technical service so we can arrange providing a free sample of this antibody.
Answered by: Tech Support Europe
Date published: 2020-07-08

Hi, can PECAM-1 Anticuerpo (H-3) sc-376764 be used with mouse tissues in histochemistry without any background?

Asked by: Fernandog
Thank you for your question. PECAM Antibody (H-3): sc-376764 is suitable for the detection of PECAM in mouse samples. Depending on the sample, antigen demasking may be necessary. You can find our recommended protocol for immunofluorescence, IHC and different antigen demasking methods by following this link: https://www.scbt.com/scbt/de/resources/protocols/immunofluorescence-cell-staining
Answered by: Tech Service
Date published: 2019-05-20

Is PECAM1 antibody (H3) conjugated to Alexa Fluor 594 stable to fixation and the subsequent solvents such as ethanol and xylene? If kept dark will it still fluoresce?

Asked by: Barb
Thank you for your question. Yes, the Alexa Fluor 594 conjugated antibody is a bright, red-fluorescent dye that provides stable imaging for immunofluorescent staining applications. Alexa Fluor 594 dye is pH-insensitive over a wide molar range. We recommend to perform all steps in the dark. Please see our recommended protocol below for details. https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2018-12-12

Is this antibody suitable for IHC of frozen sections, specifically frozen rat brain?

Asked by: harding
Thank you for your question. PECAM-1 (H-3): sc-376764 is recommended for use with frozen tissue sections by IHC. PECAM-1 (H-3) has been demonstrated in several publications to stain rat tissue by IHC.
Answered by: Technical Support
Date published: 2018-12-05
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Rated 5 out of 5 by from Ottimo funzionamento!Ho provato questo ab in WB su lisati da tessuto murino e funziona molto bene!
Date published: 2025-06-04
Rated 4 out of 5 by from It works in IFI tried this antibody in frozen section of mouse mesenteric arteries and it worked at 1:50 dilution
Date published: 2023-03-08
Rated 4 out of 5 by from Works wellI used the dilution with 1:100 for IF in mouse brain, without any antigen retrieval processes. When I tried the antigen retrieval with Hcl/ heating, it stained but not as good as the tissue stained normally. If you are doing the IF, then I recommend not to use the antigen retrieval.
Date published: 2022-11-15
Rated 1 out of 5 by from Did not work on rat brain tissueWe tested this antibody at a range of dilutions on frozen rat brain tissue fixed with either methanol or 4% paraformaldehyde and observed no staining.
Date published: 2022-01-20
Rated 5 out of 5 by from Very good antibodyWe have used this antibody in WB and worked very well at 1:1000.
Date published: 2020-11-30
Rated 4 out of 5 by from A-FABP (B-4) antibody sc-271529It is a great Ab which I got as a free sample five month ago.
Date published: 2019-12-06
Rated 5 out of 5 by from Excellent specificitySanta Cruz Biotechnology gives us a sample of its well characterized monoclonal PECAM-1 antibody two months ago and we have obtained excellent results
Date published: 2019-02-18
Rated 5 out of 5 by from Great for WBIt works very well on HeLa cell extract. Dilution 1:500.
Date published: 2018-10-08
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CD31/PECAM-1 Anticorpo (H-3) is rated 4.3 out of 5 by 43.
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