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Parafibromin Antibody (A-8) - Western Blotting - Image 361774
Parafibromin Antibody (A-8): sc-271877. Western blot analysis of Parafibromin expression in MDA-MB-231 (A), HeLa (B) and A-10 (C) whole cell lysates.
Parafibromin Antibody (A-8): sc-271877. Western blot analysis of Parafibromin expression in MDA-MB-231 (A), HeLa (B) and A-10 (C) whole cell lysates.

Parafibromin Antibody (A-8): sc-271877

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Datasheets
  • Parafibromin Antibody (A-8) is a mouse monoclonal IgG2b κ provided at 200 µg/ml
  • raised against amino acids 232-531 mapping at the C-terminus of Parafibromin of human origin
  • recommended for detection of Parafibromin of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and canine and bovine
  • See Parafibromin (2H1): sc-33638 for Parafibromin antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • m-IgG Fc BP-HRP is the preferred secondary detection reagent for Parafibromin Antibody (A-8) for WB applications. This reagent is now offered in a bundle with Parafibromin Antibody (A-8) (see ordering information below).
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    SEE ALSO...

    Parafibromin Antibody (A-8) is a mouse monoclonal IgG2b antibody that detects Parafibromin in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Parafibromin, encoded by the CDC73 gene, plays a critical role in the regulation of gene expression and is a key component of the RNA polymerase II/Paf1 complex, which is essential for histone modification and transcriptional regulation. Parafibromin′s involvement in histone modification is particularly important as anti-Parafibromin antibody (A-8) influences chromatin structure and gene accessibility, thereby impacting cellular processes such as differentiation and proliferation. The CDC73 gene, located on chromosome 1q25, is also associated with hyperparathyroidism-jaw tumor syndrome, highlighting the clinical significance of Parafibromin as a potential biomarker for diagnosing parathyroid carcinoma. Parafibromin (A-8) antibody provides a valuable tool for researchers studying the molecular mechanisms underlying parathyroid tumors and the broader implications of histone modification in cancer biology.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

    LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

    Parafibromin Antibody (A-8) References:

    1. Parafibromin, product of the hyperparathyroidism-jaw tumor syndrome gene HRPT2, regulates cyclin D1/PRAD1 expression.  |  Woodard, GE., et al. 2005. Oncogene. 24: 1272-6. PMID: 15580289
    2. The HRPT2 tumor suppressor gene product parafibromin associates with human PAF1 and RNA polymerase II.  |  Yart, A., et al. 2005. Mol Cell Biol. 25: 5052-60. PMID: 15923622
    3. Loss of parafibromin expression in a subset of parathyroid adenomas.  |  Juhlin, C., et al. 2006. Endocr Relat Cancer. 13: 509-23. PMID: 16728578
    4. SHP2 tyrosine phosphatase converts parafibromin/Cdc73 from a tumor suppressor to an oncogenic driver.  |  Takahashi, A., et al. 2011. Mol Cell. 43: 45-56. PMID: 21726809
    5. Expression of parafibromin in major renal cell tumors.  |  Cui, C., et al. 2012. Eur J Histochem. 56: e39. PMID: 23361235
    6. Effects of parafibromin expression on the phenotypes and relevant mechanisms in the DLD-1 colon carcinoma cell line.  |  Zhao, S., et al. 2013. Asian Pac J Cancer Prev. 14: 4249-54. PMID: 23991985
    7. Parafibromin Is a Component of IFN-γ-Triggered Signaling Pathways That Facilitates JAK1/2-Mediated Tyrosine Phosphorylation of STAT1.  |  Wei, J., et al. 2015. J Immunol. 195: 2870-8. PMID: 26232434
    8. Significance of Parafibromin Expression in Laryngeal Squamous Cell Carcinomas.  |  Cho, I., et al. 2016. J Pathol Transl Med. 50: 264-9. PMID: 27334641
    9. Distinct roles of parafibromin in the extracellular environment, cytoplasm and nucleus of osteosarcoma cells.  |  Zhu, JJ., et al. 2016. Am J Transl Res. 8: 2426-31. PMID: 27347351
    10. Parafibromin immunostainings of parathyroid tumors in clinical routine: a near-decade experience from a tertiary center.  |  Juhlin, CC., et al. 2019. Mod Pathol. 32: 1082-1094. PMID: 30923346

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    Parafibromin Antibody (A-8)

    sc-271877
    200 µg/ml
    		$322.00

    Parafibromin Antibody (A-8): m-IgG Fc BP-HRP Bundle

    sc-540271
    200 µg Ab; 10 µg BP
    		$361.00

    What is the recommended fixation method for immunofluorescence staining with Parafibromin (A-8): sc-271877 monoclonal antibody?

    Asked by: jerojero
    Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
    Answered by: Technical Support
    Date published: 2017-02-25
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    Rated 5 out of 5 by from Good for both Western blot and co-IPGood antibody. Good for both Western blot and co-IP
    Date published: 2017-01-16
    Rated 5 out of 5 by from Superb immunofluorescence nuclear stainingSuperb immunofluorescence nuclear staining in methanol-fixed HeLa cells. -SCBT QC
    Date published: 2015-01-05
    Rated 5 out of 5 by from Good Western Blot data of Parafibromin expressionGood Western Blot data of Parafibromin expression in non-transfected 293T, mouse Parafibromin transfected 293T and C32 whole cell lysates. -SCBT QC
    Date published: 2013-11-30
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    Parafibromin Antibody (A-8) is rated 5.0 out of 5 by 3.
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