p-Dynamin I Antibody (F-11): sc-377568

p-Dynamin I Antibody (F-11) is a mouse monoclonal IgG2b kappa light chain antibody that detects Ser 795 phosphorylated Dynamin I in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-p-Dynamin I antibody (F-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Dynamin I plays a crucial role as a GTPase enzyme in the retrieval of synaptic vesicles following exocytosis, which is essential for maintaining synaptic function and neurotransmission. Dynamin I is primarily located in the cytosolic compartment of nerve terminals, where phosphorylation occurs at Serine 795 by protein kinase C (PKC). This specific phosphorylation event is significant as Dynamin I′s interaction with other molecules influences the SH3 domain of p85, the regulatory subunit of phosphatidylinositol 3-kinase. Dynamin I′s phosphorylation state is critical for function, as dephosphorylation is necessary for effective synaptic vesicle retrieval, indicating that phosphorylation status can modulate subcellular localization and activity.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

p-Dynamin I Antibody (F-11) References:

1. Phosphorylation of dynamin I on Ser-795 by protein kinase C blocks its association with phospholipids.  |  Powell, KA., et al. 2000. J Biol Chem. 275: 11610-7. PMID: 10766777
2. Protein phosphorylation is required for endocytosis in nerve terminals: potential role for the dephosphins dynamin I and synaptojanin, but not AP180 or amphiphysin.  |  Cousin, MA., et al. 2001. J Neurochem. 76: 105-16. PMID: 11145983
3. Improved detection of hydrophilic phosphopeptides using graphite powder microcolumns and mass spectrometry: evidence for in vivo doubly phosphorylated dynamin I and dynamin III.  |  Larsen, MR., et al. 2004. Mol Cell Proteomics. 3: 456-65. PMID: 14762214
4. The in vivo phosphorylation sites of rat brain dynamin I.  |  Graham, ME., et al. 2007. J Biol Chem. 282: 14695-707. PMID: 17376771
5. Dephosphin, a 96,000 Da substrate of protein kinase C in synaptosomal cytosol, is phosphorylated in intact synaptosomes.  |  Robinson, PJ. 1991. FEBS Lett. 282: 388-92. PMID: 2037055
6. Differential phosphorylation of dynamin I isoforms in subcellular compartments demonstrates the hidden complexity of phosphoproteomes.  |  Chan, LS., et al. 2010. J Proteome Res. 9: 4028-37. PMID: 20560669
7. Phosphorylation and nitration of tyrosine residues affect functional properties of Synaptophysin and Dynamin I, two proteins involved in exo-endocytosis of synaptic vesicles.  |  Mallozzi, C., et al. 2013. Biochim Biophys Acta. 1833: 110-21. PMID: 23103755
8. Disappearance and reformation of synaptic vesicle membrane upon transmitter release observed under reversible blockage of membrane retrieval.  |  Koenig, JH. and Ikeda, K. 1989. J Neurosci. 9: 3844-60. PMID: 2573698
9. Dynamin I is a Ca(2+)-sensitive phospholipid-binding protein with very high affinity for protein kinase C.  |  Liu, JP., et al. 1994. J Biol Chem. 269: 21043-50. PMID: 8063723
10. Dynamin GTPase regulated by protein kinase C phosphorylation in nerve terminals.  |  Robinson, PJ., et al. 1993. Nature. 365: 163-6. PMID: 8371759

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-Dynamin I Antibody (F-11) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-p-Dynamin I Antibody (F-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-Dynamin I Antibody (F-11) Specifications

Alternate Product Name: Dynamin I

Product Clone Name: F-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: Ser 795

Epitope Target: Dynamin I

Epitope Species: human

Product Reactivity/Detection: Ser 795 phosphorylated Dynamin I

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for p-Dynamin I Antibody (F-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred Dynamin I antibody is Dynamin I Antibody (D5): sc-12724.