Nidogen Antibody (2X59): sc-59870

Nidogen Antibody (2X59) is a rat monoclonal IgG2a antibody that detects Nidogen in mouse samples through immunofluorescence (IF) applications. Nidogen, also known as Entactin, is a crucial sulfated glycoprotein that plays a significant role in the formation and stability of basement membranes, which are essential extracellular matrices produced during embryogenesis. Nidogen acts as a vital link between key components of the extracellular matrix, specifically Laminin 1 and Collagen Type IV, facilitating their assembly into a functional structure. Nidogen′s importance lies in its highly conserved nature and ability to interact with other extracellular matrix proteins, such as Collagens Type I and IV and Perlecan, which are critical for maintaining tissue integrity and cellular adhesion. Notably, Nidogen is synthesized and secreted by mesenchymal peritubular cells and myoepithelial cells, influencing cell adhesion in an autocrine manner. Nidogen expression is tightly regulated during embryonic and fetal development, being present exclusively in fully developed basement membranes of the ectoderm, while absent in the developing endodermal basement membrane. Anti-Nidogen antibody (2X59) is an invaluable tool for researchers studying the dynamics of basement membrane assembly and Nidogen′s role in various developmental processes.

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Nidogen Antibody (2X59) References:

1. Urokinase binding to laminin-nidogen. Structural requirements and interactions with heparin.  |  Stephens, RW., et al. 1992. Eur J Biochem. 207: 937-42. PMID: 1499567
2. Recombinant nidogen consists of three globular domains and mediates binding of laminin to collagen type IV.  |  Fox, JW., et al. 1991. EMBO J. 10: 3137-46. PMID: 1717261
3. A synaptic nidogen: developmental regulation and role of nidogen-2 at the neuromuscular junction.  |  Fox, MA., et al. 2008. Neural Dev. 3: 24. PMID: 18817539
4. Human nidogen gene: structural and functional characterization of the 5'-flanking region.  |  Fazio, MJ., et al. 1991. J Invest Dermatol. 97: 281-5. PMID: 1906509
5. Preparation of laminin/nidogen adsorbed urinary bladder decellularized materials via a mussel-inspired polydopamine coating for pelvic reconstruction.  |  Ge, L., et al. 2017. Am J Transl Res. 9: 5289-5298. PMID: 29312483
6. Analysis of degradation of the basement membrane protein nidogen, using a specific monoclonal antibody.  |  Dziadek, M., et al. 1988. Eur J Biochem. 172: 219-25. PMID: 3126070
7. Abnormal promoter DNA hypermethylation of the integrin, nidogen, and dystroglycan genes in breast cancer.  |  Strelnikov, VV., et al. 2021. Sci Rep. 11: 2264. PMID: 33500458
8. Expression of laminin and nidogen genes during the postimplantation development of the mouse placenta.  |  Thomas, T. and Dziadek, M. 1993. Biol Reprod. 49: 1251-9. PMID: 8286607
9. Ascidian entactin/nidogen. Implication of evolution by shuffling two kinds of cysteine-rich motifs.  |  Nakae, H., et al. 1993. Eur J Biochem. 213: 11-9. PMID: 8477687
10. Importance of nidogen binding to laminin gamma1 for branching epithelial morphogenesis of the submandibular gland.  |  Kadoya, Y., et al. 1997. Development. 124: 683-91. PMID: 9043083

The preferred Nidogen antibody is Nidogen Antibody (C-7): sc-133175.