Nidogen Antibody (2Q583): sc-71700

Nidogen Antibody (2Q583) is a rat monoclonal IgG2a antibody that detects Nidogen in mouse, rat, and human samples through applications such as western blotting (WB) and immunoprecipitation (IP). Nidogen, a crucial component of the extracellular matrix, plays a significant role in the structural integrity and function of basement membranes, which are essential for tissue organization and cellular signaling. Anti-Nidogen antibody (2Q583) targets proteins primarily located in the basement membranes of ectoderm-derived tissues, where Nidogen serves as a vital link between other matrix components, such as Laminin and Collagen Type IV, facilitating their assembly and stability. The interaction of Nidogen with these proteins is critical for maintaining the architecture of the extracellular matrix, which in turn influences cell behavior, migration, and differentiation. Additionally, Nidogen undergoes extensive post-translational modifications, including glycosylation, which are important for stability and function. Nidogen presence is particularly important during embryonic and fetal development, as Nidogen ensures proper formation of basement membranes, thereby supporting the development of various tissues and organs. Nidogen (2Q583) antibody enables researchers to gain valuable insights into the dynamics of basement membrane assembly and its implications in developmental biology and disease processes.

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Nidogen Antibody (2Q583) References:

1. Mesenchymal entactin-1 (nidogen-1) is required for adhesion of peritubular cells of the rat testis in vitro.  |  Konrad, L., et al. 2000. Eur J Cell Biol. 79: 112-20. PMID: 10727019
2. Inhibition of basement membrane formation by a nidogen-binding laminin gamma1-chain fragment in human skin-organotypic cocultures.  |  Breitkreutz, D., et al. 2004. J Cell Sci. 117: 2611-22. PMID: 15159456
3. Contribution of ADAMTS1 as a tumor suppressor gene in human breast carcinoma. Linking its tumor inhibitory properties to its proteolytic activity on nidogen-1 and nidogen-2.  |  Martino-Echarri, E., et al. 2013. Int J Cancer. 133: 2315-24. PMID: 23681936
4. Epithelial basement membrane proteins perlecan and nidogen-2 are up-regulated in stromal cells after epithelial injury in human corneas.  |  Torricelli, AA., et al. 2015. Exp Eye Res. 134: 33-8. PMID: 25797478
5. Nidogen and heparan sulfate proteoglycan: detection of newly isolated basement membrane components in normal and epidermolysis bullosa skin.  |  Caughman, SW., et al. 1987. J Invest Dermatol. 89: 547-50. PMID: 2960746
6. The more potential performance of nidogen 2 methylation by tissue or plasma DNA over brichoalveolar lavage DNA in diagnosis of nonsmall cell lung cancer.  |  Feng, X., et al. 2018. J Cancer Res Ther. 14: S341-S346. PMID: 29970687
7. Binding properties and protease stability of recombinant human nidogen.  |  Mayer, U., et al. 1995. Eur J Biochem. 227: 681-6. PMID: 7867626
8. Structure and localization of O- and N-linked oligosaccharide chains on basement membrane protein nidogen.  |  Fujiwara, S., et al. 1993. Matrix. 13: 215-22. PMID: 8326911
9. Abnormal expression and distribution of nidogen in Hirschsprung's disease.  |  Parikh, DH., et al. 1995. J Pediatr Surg. 30: 1687-93. PMID: 8749925
10. Meprin A, the major matrix degrading enzyme in renal tubules, produces a novel nidogen fragment in vitro and in vivo.  |  Walker, PD., et al. 1998. Kidney Int. 53: 1673-80. PMID: 9607199

The preferred Nidogen antibody is Nidogen Antibody (C-7): sc-133175.