Date published: 2025-9-9
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Mucin 2/MUC2 Antibody (Ccp58) - Immunohistochemistry - Image 160065
Anticorpo Mucina 2 (Ccp58): sc-7314. Coloração por imunoperoxidase de tecido do cólon humano normal fixado em formalina e incluído em parafina, mostrando a coloração da Mucina 2 segregada.
Anticorpo Mucina 2 (Ccp58): sc-7314. Coloração por imunoperoxidase de tecido do cólon humano normal fixado em formalina e incluído em parafina, mostrando a coloração da Mucina 2 segregada.

Mucin 2/MUC2 Anticorpo (Ccp58): sc-7314

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Fichas de dados
  • Mucin 2/MUC2 Anticorpo Ccp58é um anticorpo monoclonal produzido em camundongo IgG1 κ, citado em 87 publicações, fornecido em 200 µg/ml
  • ativado contra um péptido sintético que codifica 5 repetições em tandem de Mucin 2 de origem human
  • Anti-Mucin 2/MUC2 Anticorpo (Ccp58) é recomendado para a detecção de Mucin 2 de mouse, rat e human origem em IF e IHC(P)
  • também disponível conjugado com Alexa Fluor® 488 (sc-7314 AF488) ou Alexa Fluor® 594 (sc-7314 AF594), 200 µg/ml, para IF, IHC(P) e FCM
  • também disponível conjugado com Alexa Fluor® 680 ou Alexa Fluor® 790 para WB (NIR), IF e FCM; para utilização com sistemas de deteção de infravermelhos próximos (NIR), tais como LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure e outros sistemas comparáveis
  • Entre em contato conosco e receba GRATUITAMENTE um amostra de 10 µg de Mucin 2/MUC2 (Ccp58): sc-7314.
  • m-IgG Fc BP-HRP e 1 BP-HRP">m-IgG1 BP-HRP são os reagentes de deteção secundários preferidos para Mucin 2/MUC2 Antibody (Ccp58) for IHC(P) applications. Estes reagentes são agora oferecidos em conjuntos com Mucin 2/MUC2 Antibody (Ccp58)(ver informações de encomenda abaixo).

LINKS RÁPIDOS

Produtos auxiliares
Descricao
Identificacao do gene
Ficha de dados e Protocolos
Informações de investigação

VEJA TAMBÉM

Adicional Mucin Anticorpos incluindo Mucin 1, Mucin 12, Mucin 13, Mucin 15, Mucin 16, Mucin 2, Mucin 3, Mucin 4, Mucin 5AC, Mucin 5B, Mucin 6, Mucin 7 e Epithelial sialomucin

O anticorpo Mucin 2/MUC2 (Ccp58) é um anticorpo monoclonal IgG1 κ de camundongo contra Mucina 2 que detecta Mucina 2 de origem de camundongo, rato e humana por IF e IHC(P). O anticorpo Mucin 2/MUC2 (Ccp58) está disponível nas formas conjugada e não conjugada de anticorpo anti-Mucin 2. As mucinas são uma família de proteínas secretadas altamente glicosiladas, com uma estrutura básica que consiste em um número variável de repetições em tandem (VNTRs) codificadas por 60 pares de bases (Mucina 1), 69 pares de bases (Mucina 2) e 51 pares de bases (Mucina 3). O número de repetições é altamente polimórfico e varia entre diferentes alelos. As proteínas da mucina 1 são expressas como proteínas de membrana do tipo I, além das formas secretadas. A mucina 1 é expressa de forma aberrante em tumores epiteliais, inclusive carcinomas de mama. A mucina 2 reveste os epitélios dos intestinos e das vias aéreas e está associada a tumores do cólon. A mucina 3 é um dos principais componentes de vários géis de muco e é amplamente expressa em células normais e tumorais.

Para uso em exclusivo em pesquisa. Não se destina a uso em diagnostico e tratamento.

Alexa Fluor® é uma marca comercial da Molecular Probes Inc., OR., EUA

LI-COR® e Odyssey® são marcas registadas da LI-COR Biosciences

Referencias do Mucin 2/MUC2 Anticorpo (Ccp58):

  1. Os oligossacáridos ligados a N desempenham um papel na dimerização dependente de dissulfureto da mucina intestinal Muc2.  |  Bell, SL., et al. 2003. Biochem J. 373: 893-900. PMID: 12744721
  2. A mucina intestinal humana MUC2 tem subdomínios ricos em cisteína localizados tanto a montante como a jusante da sua região central repetitiva.  |  Gum, JR., et al. 1992. J Biol Chem. 267: 21375-83. PMID: 1400449
  3. O gene humano da mucina MUC2 é regulado transcritivamente por proteínas de homeodomínio Cdx em linhas celulares de carcinoma gastrointestinal.  |  Mesquita, P., et al. 2003. J Biol Chem. 278: 51549-56. PMID: 14525978
  4. O gene murino da mucina Muc2 é regulado por transcrição pelo fator de transcrição GATA-4 com zinco nas células intestinais.  |  van der Sluis, M., et al. 2004. Biochem Biophys Res Commun. 325: 952-60. PMID: 15541382
  5. Clonagem molecular de cDNAs derivados de um novo gene de mucina intestinal humana.  |  Gum, JR., et al. 1990. Biochem Biophys Res Commun. 171: 407-15. PMID: 2393399
  6. Clonagem e sequenciação de um cDNA de mucina de tumor pancreático humano.  |  Lan, MS., et al. 1990. J Biol Chem. 265: 15294-9. PMID: 2394722
  7. Isolamento e sequenciação de um cDNA que codifica o antigénio associado ao carcinoma da mama DF3 humano.  |  Siddiqui, J., et al. 1988. Proc Natl Acad Sci U S A. 85: 2320-3. PMID: 2895474
  8. A nocaute da mucina-2 é um modelo de defeitos de junção intercelular, danos mitocondriais e depleção de ATP no epitélio intestinal.  |  Borisova, MA., et al. 2020. Sci Rep. 10: 21135. PMID: 33273633
  9. A eliminação da mucina 2 induz colite com anomalias metabólicas concomitantes em ratinhos.  |  Ye, J., et al. 2021. Am J Physiol Gastrointest Liver Physiol. 320: G791-G803. PMID: 33728986
  10. MUC2 e factores bacterianos relacionados: Alvos terapêuticos para a colite ulcerosa.  |  Yao, D., et al. 2021. EBioMedicine. 74: 103751. PMID: 34902790
  11. Perfis de Expressão de Mucina na Colite Ulcerosa: New Insights on the Histological Mucosal Healing.  |  Leoncini, G., et al. 2024. Int J Mol Sci. 25: PMID: 38339134
  12. Associação do antigénio do cancro da mama DF3/MUC1 com Grb2 e a proteína de troca Sos/Ras.  |  Pandey, P., et al. 1995. Cancer Res. 55: 4000-3. PMID: 7664271

Informacoes sobre ordens

Nome do ProdutoNumero de CatalogoUNIDPrecoQdeFAVORITOS

Mucin 2/MUC2 Anticorpo (Ccp58)

sc-7314
200 µg/ml
$316.00

Pacote do Mucin 2/MUC2 (Ccp58): m-IgG Fc BP-HRP

sc-526497
200 µg Ab; 10 µg BP
$354.00

Pacote do Mucin 2/MUC2 (Ccp58): m-IgG1 BP-HRP

sc-531870
200 µg Ab; 20 µg BP
$354.00

Mucin 2/MUC2 Anticorpo (Ccp58) Alexa Fluor® 488

sc-7314 AF488
200 µg/ml
$357.00

Mucin 2/MUC2 Anticorpo (Ccp58) Alexa Fluor® 546

sc-7314 AF546
200 µg/ml
$357.00

Mucin 2/MUC2 Anticorpo (Ccp58) Alexa Fluor® 594

sc-7314 AF594
200 µg/ml
$357.00

Mucin 2/MUC2 Anticorpo (Ccp58) Alexa Fluor® 680

sc-7314 AF680
200 µg/ml
$357.00

Mucin 2/MUC2 Anticorpo (Ccp58) Alexa Fluor® 790

sc-7314 AF790
200 µg/ml
$357.00

Does this MUC2 antibody have cross-reactivity with MUC1 ?

Asked by: jm123
Thank you for your question. The antibody has been known to only detect MUC2. https://www.uniprot.org/uniprotkb/Q02817/entry#sequences
Answered by: BlakeJ
Date published: 2024-04-15

i received sc-7314 at room temperature though it says to store at 4C. Will antibody integrity be compromised? also- do you have a CoA for this item? Thank you.

Asked by: jujuB
Some of our antibodies ship out ambient. They can last up to 5 days out of storage. We do not have CoAs for our antibodies.
Answered by: Technical Service
Date published: 2023-12-18

I tried using this antibody for IF at a 1:50 and 1:100 dilution in 5% BSA to stain for MUC-2 secreted by HT29-MTX cells but it did not work well.  Is there an antibody that is better suited for this application using HT29-MTX cells?

Asked by: isramire
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext. 1, allowing for a more interactive discussion of this and other related questions.”
Answered by: Blake J
Date published: 2021-11-17

The Rabbit polyclonal muc2 primary antibody H-300 Santa Cruz technologies was been discontinued. Is there an alternative MUC2 antibody that can be used to stain mouse muc2 for IF. What would be the Alexa488 secondary antibody to use?

Asked by: Anonymous
Yes, this antibody sc-7314 should be suitable for IF on mouse tissues. Please contact technical support for further assistance.
Answered by: Tech Service 1
Date published: 2020-11-16

What concentration do you recommend for this antibody for mouse detection via IF and IHC?

Asked by: crrivet
Thank you for your question. The recommended starting dilution is 1:50 (range 1:50-1:500).
Answered by: Technical Support
Date published: 2019-09-27

Will this antibody work in a Sandwich Elisa?

Asked by: KathrinR
Thank you for your question. We have not tested this antibody in an ELISA and so this is not a recommended application at this time.
Answered by: Technical Support
Date published: 2019-04-26

This might be a novice question, however, shouldn't a primary antibody never be from the same host as the target species? Nonetheless, you suggest using this mouse monoclonal antibody to detect mucin 2 in mice. Could you elaborate how this works?

Asked by: Mwolter
Thank you for your question. There is no problem with using a mouse monoclonal antibody for detection of the corresponding protein in mouse cells or tissues. The main concern would be that your secondary antibody (anti-mouse IgG) may cross react non-specifically with endogenous mouse IgG expressed in your samples. This is only really an issue if your samples are rich in endogenous IgG. In that case, we would recommend optimizing your secondary antibody, blocking and incubation conditions. Alternatively, you can always use a directly conjugated primary antibody and avoid using any secondary antibody for detection.
Answered by: Technical Support
Date published: 2019-04-13

Will this antibody cross-react in my zebrafish samples?

Asked by: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 4
Date published: 2017-01-13
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Rated 3 out of 5 by from It works well in WBWe tested this Ab by WB at 1:1000 dilution. Strong band and no background. We'll also test it in IF on Human Intestinal Colonoids.
Date published: 2023-03-22
Rated 1 out of 5 by from do not work on IHCdo not work on IHC-DAB and IHC-IF in mouse small intestine and large intestine. dilution 1:50.
Date published: 2020-08-17
Rated 1 out of 5 by from Does not stain goblet cells in murine intestine IFWe purchased two lots of this antibody and they do not stain for Mucin2, instead they appear to stain extracellular matrix or some other protein in the basal lamina propria (attached). We believe this is lot specific (attached), however customer service has chosen to ignore this after we politely brought it to their attention. We do not recommend purchasing this antibody, and are disappointed but not surprised by santa cruz's response to our concerns. We are however amused by the staff 5-star ratings of this antibody, fair play.
Date published: 2019-11-14
Rated 4 out of 5 by from 使用方便，功能齐全我在一个月前购买了mucin-2 此产品，并用于黏液层免疫组化染色，实验效果显著,这个产品使用方便，且抗体效价很高。
Date published: 2019-06-08
Rated 1 out of 5 by from No effect on mouse colon IF stainingNo effect on colonic IF staining in mice, concentration 1:100
Date published: 2019-05-11
Rated 1 out of 5 by from Mouse small intestine tissueDoesn't work for mouse small intestine tissue via IF on cryosections & IF on mouse small intestinal organoids.
Date published: 2018-07-08
Rated 5 out of 5 by from strong bandNo non-specific binding and background ,just strong band,I am not recommend use Tween-20 when blocking the membrane,it may cause background.
Date published: 2017-07-01
Rated 5 out of 5 by from specific stainingWe use Mucin 2 antibody by Immunohistochemistry of human tonsil tissue stained with Anti-Mucin 2 antibody , we got the specific staining.
Date published: 2017-05-28
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Mucin 2/MUC2 Anticorpo (Ccp58) is rated 3.8 out of 5 by 16.
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