β-Actina (C4): sc-47778. Análise por western blot de infravermelhos próximos da expressão de β-Actina em lisados de células inteiras de HeLa (A), MCF7 (B) e A-431 (C). Bloqueado com Reagente de Bloqueio UltraCruz®: sc-516214. Reagente de deteção utilizado: m-IgG Fc BP-CFL 790: sc-533658.
β-Actina (C4): sc-47778. Análise por western blot de infravermelhos próximos da expressão de β-Actina em lisados de células inteiras de HeLa (A), MCF7 (B) e A-431 (C). Bloqueado com Reagente de Bloqueio UltraCruz®: sc-516214. Reagente de deteção utilizado: m-IgG Fc BP-CFL 790: sc-533658.
mouse IgG Fc binding protein (m-IgG Fc BP) conjugated to CruzFluor™ 790 (CFL 790)
fornecidos a 50 µg em 0.5 ml de volume
CruzFluor™ 790 (CFL 790) is a near infrared fluorescent dye that is an excellent substitute for Alexa Fluor® 790, offering comparable photostability and the ability to resist protein quenching
Excitation of 786 nm, Emission of 811 nm
Suitable for use with Near-Infrared (NIR) imaging systems, such as LI-COR/Odyssey, Invitrogen/iBright and other comparable systems
Strongly recommended alternative to conventional anti-mouse IgG secondary antibodies for Near-Infrared Western blotting (WB [NIR]), Immunofluorescence (IF) and Flow Cytometry (FCM)
Suitable for binding to the Fc region of many, but not all, mouse IgG1, IgG2a and IgG2b immunoglobulins, and to a lesser extent, to mouse IgG3; not suitable for use with mouse monoclonal IgM, IgA or IgE antibodies
Not cross-reactive with human, rat, rabbit or goat IgG antibodies
Highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies
Mouse IgG binding proteins are recommended for some, but not all of our monoclonal antibodies. Product descriptions on our monoclonal antibody product pages will state if the Mouse IgG binding protein is the preferred detection reagent for that product.
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Para uso em exclusivo em pesquisa. Não se destina a uso em diagnostico e tratamento.