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GAPDH (0411): sc-47724. Análise por western blot de infravermelhos próximos da expressão de GAPDH em lisados de células inteiras de Jurkat (A), MOLT-4 (B), HeLa (C), K-562 (D), BJAB (E) e IMR-32 (F). Bloqueado com Reagente de Bloqueio UltraCruz®: sc-516214. Reagente de deteção utilizado: m-IgG Fc BP-CFL 680: sc-533657.
m-IgG Fc BP-CFL 680: sc-533657
- mouse IgG Fc binding protein (m-IgG Fc BP) conjugated to CruzFluor™ 680 (CFL 680)
- fornecidos a 50 µg em 0.5 ml de volume
- CruzFluor™ 680 (CFL 680) is a near infrared fluorescent dye that is an excellent substitute for Alexa Fluor® 680, offering comparable photostability and the ability to resist protein quenching
- Excitation of 683 nm, Emission of 700 nm
- Suitable for use with Near-Infrared (NIR) imaging systems, such as LI-COR/Odyssey, Invitrogen/iBright and other comparable systems
- Strongly recommended alternative to conventional anti-mouse IgG secondary antibodies for Near-Infrared Western blotting (WB [NIR]), Immunofluorescence (IF) and Flow Cytometry (FCM)
- Suitable for binding to the Fc region of many, but not all, mouse IgG1, IgG2a and IgG2b immunoglobulins, and to a lesser extent, to mouse IgG3; not suitable for use with mouse monoclonal IgM, IgA or IgE antibodies
- Not cross-reactive with human, rat, rabbit or goat IgG antibodies
- Highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies
- Mouse IgG binding proteins are recommended for some, but not all of our monoclonal antibodies. Product descriptions on our monoclonal antibody product pages will state if the Mouse IgG binding protein is the preferred detection reagent for that product.
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Para uso em exclusivo em pesquisa. Não se destina a uso em diagnostico e tratamento.
Alexa Fluor® é uma marca comercial da Molecular Probes Inc., OR., EUA
LI-COR® e Odyssey® são marcas registadas da LI-COR Biosciences
Informacoes sobre ordens
| Nome do Produto | Numero de Catalogo | UNID | Preco | Qde | FAVORITOS | |
m-IgG Fc BP-CFL 680 | sc-533657 | 50 µg/0.5 ml | $92.00 |