GAPDH (0411): sc-47724. Análise fluorescente por western blot da expressão de GAPDH em lisados de células inteiras de Jurkat (A), MOLT-4 (B), HeLa (C), BJAB (D) e IMR-32 (E). Bloqueado com Reagente de Bloqueio UltraCruz®: sc-516214. Reagente de deteção utilizado: m-IgG Fc BP-CFL 555: sc-533654.
GAPDH (0411): sc-47724. Análise fluorescente por western blot da expressão de GAPDH em lisados de células inteiras de Jurkat (A), MOLT-4 (B), HeLa (C), BJAB (D) e IMR-32 (E). Bloqueado com Reagente de Bloqueio UltraCruz®: sc-516214. Reagente de deteção utilizado: m-IgG Fc BP-CFL 555: sc-533654.
mouse IgG Fc binding protein (m-IgG Fc BP) conjugated to CruzFluor™ 555 (CFL 555)
fornecidos a 50 µg em 0.5 ml de volume
CruzFluor™ 555 (CFL 555) is a orange/red fluorescent dye that is an excellent substitute for Alexa Fluor® 555, offering comparable photostability and the ability to resist protein quenching
Excitation of 556 nm, Emission of 569 nm
Suitable for use with RGB imaging systems, such as Invitrogen/iBright and other comparable systems
Substituto altamente recomendado para os anticorpos secundários convencionais IgG anti-camundongo para RGB Western blotting (WB [RGB]), Imunofluorescência (IF) e Citometria de Fluxo (FCM)
Suitable for binding to the Fc region of many, but not all, mouse IgG1, IgG2a and IgG2b immunoglobulins, and to a lesser extent, to mouse IgG3; not suitable for use with mouse monoclonal IgM, IgA or IgE antibodies
Not cross-reactive with human, rat, rabbit or goat IgG antibodies
Highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies
Mouse IgG binding proteins are recommended for some, but not all of our monoclonal antibodies. Product descriptions on our monoclonal antibody product pages will state if the Mouse IgG binding protein is the preferred detection reagent for that product.
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Para uso em exclusivo em pesquisa. Não se destina a uso em diagnostico e tratamento.
Alexa Fluor® é uma marca comercial da Molecular Probes Inc., OR., EUA