MMD CRISPR/Cas9 KO Plasmid (m): sc-426581
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MMD CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MMD genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MMD CRISPR/Cas9 KO Plasmid (m) | sc-426581 | 20 µg | $397.00 |
Overview
Mmd encodes MMD, a multi-pass membrane protein enriched at the Golgi and endomembrane system that has been linked to myeloid lineage maturation and innate immune cell biology. Studies implicate MMD in regulating membrane trafficking and signaling outputs that shape inflammatory responses, with connections to MAPK/ERK and cytokine-associated pathways in macrophages and related cell types. Altered MMD expression has been reported across immune and tumor contexts, supporting its use as a mechanistic node for investigating how myeloid programs interface with cellular stress and microenvironmental cues. In mouse models, MMD perturbation provides a handle to dissect lineage-specific signaling and organ-level inflammation phenotypes without making therapeutic or clinical inferences.
MMD CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Mmd gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Mmd together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Mmd open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MMD protein expression.
This CRISPR knockout system enables efficient generation of Mmd-deficient cell models for investigation of MMD signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Mmd exon(s) critical for MMD function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Mmd genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MMD CRISPR/Cas9 KO Plasmid (m) and MMD CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Mmd locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MMD HDR Plasmid (m) and MMD HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Mmd homology arms to support homology-directed repair at defined Mmd target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.