mAChR M2 Antibody (M2-2-B3): sc-33712
- mAChR M2 Antibody (M2-2-B3) is a rat monoclonal IgG2a mAChR M2 antibody, cited in 2 publications, provided at 200 µg/ml
- raised against i3 loop of m2 receptor fusion protein
- mAChR M2 Antibody (M2-2-B3) is recommended for detection of mAChR M2 of mouse, rat and human origin by WB, IP and IHC(frozen); not recommended for IF or paraffin-embedded sections
- Anti-mAChR M2 Antibody (M2-2-B3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for mAChR M2 Antibody (M2-2-B3). This work is in progress.
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mAChR M2 Antibody (M2-2-B3) is a rat monoclonal IgG2a antibody that detects the muscarinic acetylcholine receptor M2 (mAChR M2) protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and immunohistochemistry (frozen). mAChR M2 (M2-2-B3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. mAChR M2 plays a crucial role in the central and peripheral nervous systems by mediating inhibitory neurotransmission and modulating various physiological processes, such as heart rate and glandular secretion. Located predominantly in the heart and brain, mAChR M2 is essential for regulating cardiac function and neurotransmitter release, making mAChR M2 a significant target for understanding cardiovascular diseases and neurological disorders. mAChR M2′s ability to couple with G-proteins allows mAChR M2 to influence downstream signaling pathways, which can lead to diverse cellular responses. Given mAChR M2′s importance in both normal physiology and disease states, mAChR M2 is a valuable protein for research and therapeutic targeting, and anti-mAChR M2 antibody (M2-2-B3) serves as a reliable tool for studying expression and function in various biological contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
mAChR M2 Antibody (M2-2-B3) References:
- Distinct internalization of M2 muscarinic acetylcholine receptors confers selective and long-lasting desensitization of signaling to phospholipase C. | Krudewig, R., et al. 2000. J Neurochem. 74: 1721-30. PMID: 10737631
- Agonist mediated internalization of M2 mAChR is beta-arrestin-dependent. | Jones, KT., et al. 2006. J Mol Signal. 1: 7. PMID: 17224084
- Muscarinic acetylcholine receptors: structure and function. | Hulme, EC., et al. 1991. Biochem Soc Trans. 19: 133-8. PMID: 1903735
- Muscarinic acetylcholine receptors: typical G-coupled receptors. | Hulme, EC. 1990. Symp Soc Exp Biol. 44: 39-54. PMID: 2130518
- Molecular cloning and expression of a fifth muscarinic acetylcholine receptor. | Liao, CF., et al. 1989. J Biol Chem. 264: 7328-37. PMID: 2540186
- Primary structure and biochemical properties of an M2 muscarinic receptor. | Peralta, EG., et al. 1987. Science. 236: 600-5. PMID: 3107123
- M2 muscarinic receptors negatively modulate cell migration in human glioblastoma cells. | Guerriero, C., et al. 2024. Neurochem Int. 174: 105673. PMID: 38185384
- M2 Muscarinic Receptor Stimulation Induces Autophagy in Human Glioblastoma Cancer Stem Cells via mTOR Complex-1 Inhibition. | Guerriero, C., et al. 2023. Cancers (Basel). 16: PMID: 38201453
- Muscarinic receptors--characterization, coupling and function. | Caulfield, MP. 1993. Pharmacol Ther. 58: 319-79. PMID: 7504306
- Muscarinic acetylcholine receptor subtypes: localization and structure/function. | Brann, MR., et al. 1993. Prog Brain Res. 98: 121-7. PMID: 8248499
- Distribution of muscarinic receptor subtypes in rat brain from postnatal to old age. | Tice, MA., et al. 1996. Brain Res Dev Brain Res. 92: 70-6. PMID: 8861724
- Pharmacology of muscarinic acetylcholine receptor subtypes (m1-m5): high throughput assays in mammalian cells. | Bräuner-Osborne, H. and Brann, MR. 1996. Eur J Pharmacol. 295: 93-102. PMID: 8925880
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
mAChR M2 Antibody (M2-2-B3) | sc-33712 | 200 µg/ml | $322.00 | |||
mAChR M2 Antibody (M2-2-B3) AC | sc-33712 AC | 500 µg/ml, 25% agarose | $424.00 | |||
mAChR M2 Antibody (M2-2-B3) HRP | sc-33712 HRP | 200 µg/ml | $322.00 | |||
mAChR M2 Antibody (M2-2-B3) FITC | sc-33712 FITC | 200 µg/ml | $336.00 | |||
mAChR M2 Antibody (M2-2-B3) PE | sc-33712 PE | 200 µg/ml | $349.00 | |||
mAChR M2 Antibody (M2-2-B3) Alexa Fluor® 488 | sc-33712 AF488 | 200 µg/ml | $364.00 | |||
mAChR M2 Antibody (M2-2-B3) Alexa Fluor® 546 | sc-33712 AF546 | 200 µg/ml | $364.00 | |||
mAChR M2 Antibody (M2-2-B3) Alexa Fluor® 594 | sc-33712 AF594 | 200 µg/ml | $364.00 | |||
mAChR M2 Antibody (M2-2-B3) Alexa Fluor® 647 | sc-33712 AF647 | 200 µg/ml | $364.00 | |||
mAChR M2 Antibody (M2-2-B3) Alexa Fluor® 680 | sc-33712 AF680 | 200 µg/ml | $364.00 | |||
mAChR M2 Antibody (M2-2-B3) Alexa Fluor® 790 | sc-33712 AF790 | 200 µg/ml | $364.00 |